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Meldola’s Blue — doped titania sol-gel sensor for NADH determination

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Central European Journal of Chemistry

Abstract

Titania layers obtained by a sol-gel technique doped with redox mediator, Meldola’s Blue, were employed for construction of a new NADH senor. Optimization of preparation process as well as experimental conditions affecting the response of the sensor were examined. Under optimal conditions NADH could be determined in the wide linear range from 90 to 2300 µM with detection limit 12 µM and a high sensitivity 12.5 nA µM−1. The usefulness of developed sensor was preliminarily checked in determination of NADH forming during enzymatic oxidation of ethanol catalyzed by alcohol dehydrogenase (ADH).

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References

  1. L. Zhu, J. Zhai, R. Yang, Ch. Tian, L. Guo, Biosens. Bioelectron. 22, 2768 (2007)

    Article  CAS  Google Scholar 

  2. H. J. Schmidt, Sol-Gel Sci. Techn. 40, 115 (2006)

    Article  CAS  Google Scholar 

  3. J. Kochana, A. Gala, A. Parczewski, J. Adamski, Anal. Bioanal. Chem. 391, 1275 (2008)

    Article  CAS  Google Scholar 

  4. T. Tsujimoto, T. Yabutani, A. Sano, Y. Tani, H. Murotani, Y. Mishima, K. Maruyama, M. Yasuzawa, J. Motonaka, Anal. Sci. 23, 59 (2007)

    Article  CAS  Google Scholar 

  5. Y.D. Tanimoto de Albuquerqu, L.F. Ferreira, Anal. Chim. Acta 596, 210 (2007)

    Article  Google Scholar 

  6. P. Du, S. Liu, P. Wu, Ch. Cai, Electrochim. Acta 53, 1811 (2007)

    Article  CAS  Google Scholar 

  7. A.S. Santos, N. Duran, L.T. Kubota, Electroanalysis 17, 1103 (2005)

    Article  CAS  Google Scholar 

  8. C.R. Raj, S. Behera, Biosens. Bioelectron. 21, 949 (2005)

    Article  CAS  Google Scholar 

  9. J. Kochana, P. Nowak, A. Jarosz-Wilkołazka, M. Bieroń, Microchem. J. 89, 171 (2008)

    Article  CAS  Google Scholar 

  10. R. Vaidya, E. Wilkins, Med. Eng. Phys. 16(5), 416 (1994)

    Article  CAS  Google Scholar 

  11. S.A. Kumar, S.-M. Chen, Anal. Chim. Acta 592, 36 (2007)

    Article  CAS  Google Scholar 

  12. C.M. Maroneze, L.T. Arenas, R.S.C. Luz, E.V. Benvenutti, R. Landers, Y. Gushikem, Electrochim. Acta 53, 4167 (2008)

    Article  CAS  Google Scholar 

  13. A.S. Santos, L. Gorton, L.T. Kubota, Electroanalysis 14, 805 (2002)

    Article  CAS  Google Scholar 

  14. S. Sampath, O. Lev, J. Electroanal. Chem. 446, 57 (1998)

    Article  CAS  Google Scholar 

  15. B. Prieto-Simon, E. Fabregas, Biosens. Bioelectron. 19, 1131 (2004)

    Article  CAS  Google Scholar 

  16. B. Prieto-Simon, J. Macanas, M. Munoz, E. Fabregas, Talanta 71, 2102 (2007)

    Article  CAS  Google Scholar 

  17. A. Vasilescu, S. Andreescu, C. Bala, C.S. Litescu, T. Noguer, J.-L. Marty, Biosens. Bioelectron. 18, 781 (2003)

    Article  CAS  Google Scholar 

  18. A. Arvinte, A.M. Sesay, V. Virtanen, C. Bala, Electroanalysis 20, 2355 (2008)

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-060, Kraków, Poland

    Juliusz Adamski & Jolanta Kochana

Authors
  1. Juliusz Adamski
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  2. Jolanta Kochana
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Correspondence to Jolanta Kochana.

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Adamski, J., Kochana, J. Meldola’s Blue — doped titania sol-gel sensor for NADH determination. cent.eur.j.chem. 9, 185–191 (2011). https://doi.org/10.2478/s11532-010-0136-6

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  • DOI: https://doi.org/10.2478/s11532-010-0136-6

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