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Determination of galacturonic acid content in pectin from fruit juices by liquid chromatographydiode array detection-electrospray ionization tandem mass spectrometry

  • Research Article
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Central European Journal of Chemistry

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method is applied for the determination of galacturonic acid (GA) of pectins in different commercial fruit juices. The separation was carried out on a C18 column using precolumn derivatization with p-aminobenzoic acid (p-ABA) and UV detection at 304 nm. The identification of GA was confirmed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in positive ion mode. The concentration of GA in the samples analyzed ranged from 12.9 ± 0.5 to 49.4 ± 0.5 mgGA L−1. Amongst the samples analyzed, mango juice was found to be richest in GA content, and therefore a good source of pectins. Detection and quantification limits of the described methodology were 1.2 and 3.9 mg L−1, respectively. Quantitative GA recoveries in the beverages had a range between 90 and 98%. The results showed that the HPLC method proposed was precise and suitable for the identification and quantification of GA in commercial fruit juices.

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Authors and Affiliations

  1. REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal

    Manuela M. Moreira, Luís F. Guido & Aquiles A. Barros

  2. iBeSa — The Beverages and Health Institute, Apartado 1044, 4466-955, S. Mamede de Infesta, Portugal

    José M. Cruz

Authors
  1. Manuela M. Moreira
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  2. Luís F. Guido
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  3. José M. Cruz
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  4. Aquiles A. Barros
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Correspondence to Luís F. Guido.

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Moreira, M.M., Guido, L.F., Cruz, J.M. et al. Determination of galacturonic acid content in pectin from fruit juices by liquid chromatographydiode array detection-electrospray ionization tandem mass spectrometry. cent.eur.j.chem. 8, 1236–1243 (2010). https://doi.org/10.2478/s11532-010-0101-4

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  • DOI: https://doi.org/10.2478/s11532-010-0101-4

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