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Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

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Central European Journal of Chemistry

Abstract

The analytical method based on the high-performance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL−1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method.

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Authors and Affiliations

  1. Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

    Petr Dobiáš, Petra Pavlíková, Martin Adam, Aleš Eisner, Blanka Beňová & Karel Ventura

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  1. Petr Dobiáš
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  2. Petra Pavlíková
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  3. Martin Adam
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  4. Aleš Eisner
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  5. Blanka Beňová
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  6. Karel Ventura
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Correspondence to Martin Adam.

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Dobiáš, P., Pavlíková, P., Adam, M. et al. Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity. cent.eur.j.chem. 8, 87–95 (2010). https://doi.org/10.2478/s11532-009-0125-9

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  • DOI: https://doi.org/10.2478/s11532-009-0125-9

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