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Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field

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Central European Journal of Chemistry

Abstract

This study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies.

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Authors and Affiliations

  1. Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

    Barbara Stopa, Barbara Piekarska, Leszek Konieczny, Janina Rybarska, Anna Jagusiak & Paweł Spólnik

  2. Department of Bioinformatics and Telemedicine, Jagiellonian University Medical College, 31-530, Kraków, Poland

    Marcin Król & Irena Roterman

  3. Electron Microscopy Laboratory, University Children’s Hospital, 30-663, Kraków, Poland

    Barbara Urbanowicz

  4. Department of Measurements and Instrumentation, AGH-University of Science and Technology, 30-059, Kraków, Poland

    Piotr Piwowar

  5. Faculty of Chemistry, Jagiellonian University, 30-060, Kraków, Poland

    Krzysztof Lewiński

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  1. Barbara Stopa
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  2. Barbara Piekarska
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  3. Leszek Konieczny
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Correspondence to Barbara Stopa.

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Stopa, B., Piekarska, B., Konieczny, L. et al. Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field. cent.eur.j.chem. 8, 41–50 (2010). https://doi.org/10.2478/s11532-009-0107-y

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  • DOI: https://doi.org/10.2478/s11532-009-0107-y

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