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Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

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Central European Journal of Chemistry

Abstract

A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (p<0.001), (p<0,01), (p<0.05) in levels of retinol, retinyl palmitate and vitamin C, respectively compared to the control group levels. A significant decrease (p<0.01) in retinyl palimitate was observed in the 7,12 dimethylbenz[a] anthracene + Hypericum Perforatum L. treated group compared to the control group..

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Arts and Sciences, Yuzuncu Yil University, 65080, Van, Turkey

    Abdulkadir Levent

  2. Department of Biochemistry, Faculty of Arts and Sciences, Yuzuncu Yil University, 65080, Van, Turkey

    Suat Ekin

  3. Department of Pharmacology, Medical Faculty, Yuzuncu Yil University, 65080, Van, Turkey

    Gökhan Oto

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  1. Abdulkadir Levent
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  2. Suat Ekin
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  3. Gökhan Oto
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Correspondence to Abdulkadir Levent.

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Levent, A., Ekin, S. & Oto, G. Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection. cent.eur.j.chem. 8, 108–115 (2010). https://doi.org/10.2478/s11532-009-0101-4

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  • DOI: https://doi.org/10.2478/s11532-009-0101-4

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