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Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

  • Enas S. Alsatari
  • Mohammad AzabEmail author
  • Omar F. Khabour
  • Karem H. Alzoubi
  • May F. Sadiq
Original Papers
  • 112 Downloads

Abstract

Objectives

The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay.

Materials and Methods

Fifty waterpipe smokers and 18 healthy non-smokers volunteered to participate in the study. Additionally, 18 heavy cigarette smokers were recruited for comparison. Chromosomal aberrations (CAs) assay was used to evaluate DNA damage in the lymphocytes.

Results

The results showed that similarly to cigarette smoking, waterpipe smoking significantly increased the frequencies of CAs (p < 0.01). In addition, the frequencies of CAs increased with more waterpipe use.

Conclusions

Waterpipe smoking causes DNA damage to lymphocytes and the damage increases with more waterpipe use.

Key words

Waterpipe Smoking Cigarette Chromosomal aberrations 

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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Enas S. Alsatari
    • 1
  • Mohammad Azab
    • 2
    • 5
    Email author
  • Omar F. Khabour
    • 1
  • Karem H. Alzoubi
    • 3
  • May F. Sadiq
    • 4
  1. 1.Department of Applied Biological Sciences, Faculty of Science and ArtsJordan University of Science and TechnologyIrbidJordan
  2. 2.Department of Community Medicine and Pathology, Faculty of MedicineThe Hashemite UniversityZarqaJordan
  3. 3.Department of Clinical Pharmacy, Faculty of PharmacyJordan University of Science and TechnologyIrbidJordan
  4. 4.Department of Biology, Faculty of ScienceYarmouk UniversityIrbidJordan
  5. 5.Department of Community Medicine and Pathology, Faculty of MedicineThe Hashemite UniversityZarqaJordan

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