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Bioaccumulation study of acrylates in algae (Chlorella kessleri) by Py-GC and Py-GC/MS

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Central European Journal of Chemistry

Abstract

Acrylate monomers methylmethacrylate (MMA) and cyclohexylmethacrylate (CHMA) bioaccumulation has been determined in aquatic organism, algae (Chlorella kessleri). Algae were collected in amount of 0.4 mg and directly injected to the pyrolytical cell. In algae bodies accumulated monomers were analysed by pyrolysis gas chromatography (Py-GC) and pyrolysis gas chromatography coupled with mass spectrometry (Py-GC/MS). Traces of the accumulated monomers in algae body can be determined after 1-, 2-, 3-weeks of incubation. Maximum content of MMA was determined after 3-week of experiment, contrariwise in the case of CHMA after 2-week exposition. Relationship with pyrolysis temperature has also been studied.

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References

  1. Thomas P. Wampler: “Introduction to pyrolysis-capillary gas chromatography”, J. Chromatogr. A, Vol. 842, (1999), pp. 207–220.

    Article  CAS  Google Scholar 

  2. R. Goodacre, R. Harvey, A.S. Howell, L.W. Greenham and C.W. Noble: “An epidemiological study of Staphylococcus intermedius strains isolated from dogs, their owners and veterinary surgeons”, J. Anal. Appl. Pyrol., Vol. 44, (1997), pp. 49–64.

    Article  CAS  Google Scholar 

  3. J.K. Voorhees, F. Basile, B.M. Beverly, Ch. Abbas-Hawks, A. Hendricker, R.B. Cody and L.T. Hadfield: “The use of biomarker compounds for the identification of bacteria by pyrolysis-mass spectrometry”, J. Anal. Appl. Pyrol., Vol. 40–41, (1997), pp. 111–134.

    Article  Google Scholar 

  4. C.C. Smith, S.L. Morgan, C.D. Parks, A. Fox and D.G. Pritchard: “Chemical marker for the differentiation of group A and group B streptococci by pyrolysis-gas chromatography-mass spectrometry”, Anal. Chem., Vol. 59, (1987), pp. 1410.

    Article  CAS  Google Scholar 

  5. G. Varhegyi, M.J. Antal, M. Jakab and P. Szabo: “Kinetic modeling of biomass pyrolysis”, J. Anal. Appl. Pyrol., Vol. 42, (1997), pp. 73–87.

    Article  CAS  Google Scholar 

  6. N.D. Danielson, J.L. Glajch and L.B. Rogers: “Pyrolysis gas chromatography of enzymes”, J. Chromatogr. Sci., Vol. 16, (1978), pp. 455–461.

    CAS  Google Scholar 

  7. F.M. Menger, G.A. Epstein, D.A. Goldberg and E. Reiner: “Computer matching of pyrolysis chromatograms of pathogenic microorganisms”, Anal. Chem., Vol. 44, (1972), pp. 423–424.

    Article  CAS  Google Scholar 

  8. Y. Ishida, H. Yokoi, S. Isomura, H. Ohtani, S. Tsuge, T. Sekino, M. Nakanishi and T. Kimoto: “Correlation analysis between fatty acid compositions of zooplankter individuals, fed on different phytoplankton species by means of pyrolysis-gas chromatography combined with on-line methylation”, J. Chromatogr. B, Biomed. Sci. Appl., Vol. 716(1–2), (1998), pp. 39–45.

    Article  CAS  Google Scholar 

  9. G. Russell: “Pyrolysis mass spectrometry: a fresh approach to old problems in brown algal systematics?”, Mar. Biol., Vol. 123, (1995), pp. 153–157.

    Article  CAS  Google Scholar 

  10. J. Cejka and Z. Sobalik: “Laboratory oxidation of fossil organic matter studied by in situ infrared spectrocopy, rock-eval pyrolysis and pyrolysis gas-chromatography-mass spectrometry”, Collect. Czech. Chem. C., Vol. 62, (1997), pp. 364–374.

    Article  CAS  Google Scholar 

  11. S. Derrene, C. Largeau and E. Cassadevall: “Occurrence of tightly bound isoprenoid acids in an algal, resistant biomacromolecule: possible geochemical implications”, Org. Geochem., Vol. 17, (1991), pp. 597–602.

    Article  Google Scholar 

  12. M.A. Kruge, P.K. Mukhopadhyay and C.F.M. Lewis: “A molecular evaluation of contaminants and natural organic matter in bottom sediments from western Lake Ontario”, Org. Geochem., Vol. 29, (1998), pp. 1797–1812.

    Article  CAS  Google Scholar 

  13. Y. Ishida, H. Ohtani, S. Tsuge, T. Sekino, M. Nakanishi and T. Kimoto: “Analysis of lipid components in zooplankter individuals by reactive pyrolysis gas chromatography in the presence of organic alkali”, Biol. Sci. Space, Vol. 12(2), (1998), pp. 131–135.

    Article  CAS  Google Scholar 

  14. www.chlorella-europe.com

  15. G. Kattner and H.S.G. Fricke: “Simple gas-liquid chromatographic method for the simultaneous determination of fatty acids and alcohols in wax esters of marine organisms”, J. Chromatogr. A, Vol. 361, (1986), pp. 263.

    Article  CAS  Google Scholar 

  16. H. Araki, N. Tatarazako, K. Kishi and K. Kuroda: “Evaluation of bioaccumulation potential of 3,4,5-trichloroguaiacol in a zooplankton (Daphnia magna) by pyrolysis-GC/MS in the presence of tetramethylammonium hydroxide (TMAH)”, J. Anal. Appl. Pyrol., Vol. 55, (2000), pp. 69–80.

    Article  CAS  Google Scholar 

  17. J.S. Gift: Toxicological Review of Methylmethacrylate, U.S. Protection Agency, Washington, 1998, pp. 3–15.

    Google Scholar 

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Halás, L., Oriňák, A., Sharif, A. et al. Bioaccumulation study of acrylates in algae (Chlorella kessleri) by Py-GC and Py-GC/MS. cent.eur.j.chem. 3, 570–582 (2005). https://doi.org/10.2478/BF02479283

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  • DOI: https://doi.org/10.2478/BF02479283

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