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β-Galactosidase from Penicillium canescens. Properties and immobilization

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Central European Journal of Chemistry

Abstract

β-galactosidase from Penicillium canescens was immobilized on chitosan, sepharose-4B, foamable polyurethane and some other carriers. The highest yield of immobilization (up to 98%) was obtained by using chitosan as a carrier. The optimum pH and temperature were not significantly altered by immobilization. High stability of immobilized β-galactosidase during storage was demonstrated. Efficient lactose saccharification (over 90%) in whey was achieved by using immobilized β-galactosidase.

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Authors and Affiliations

  1. Department of Polymer Chemistry of Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania

    Saulute Budriene, Natalija Gorochovceva & Tatjana Romaskevic

  2. Institute of Biotechnology, Kashirskoe shosse 24/17, 115478, Moscow, Russia

    Lyubov V. Yugova

  3. Lithuanian Food Institute, Taikos av. 92, 3031, Kaunas, Lithuania

    Aldona Miezeliene

  4. Institute of Biotechnology, Graiciuno 8, 02241, Vilnius, Lithuania

    Gervydas Dienys & Asta Zubriene

Authors
  1. Saulute Budriene
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  2. Natalija Gorochovceva
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  3. Tatjana Romaskevic
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  4. Lyubov V. Yugova
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  5. Aldona Miezeliene
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  6. Gervydas Dienys
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  7. Asta Zubriene
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Budriene, S., Gorochovceva, N., Romaskevic, T. et al. β-Galactosidase from Penicillium canescens. Properties and immobilization. cent.eur.j.chem. 3, 95–105 (2005). https://doi.org/10.2478/BF02476241

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  • DOI: https://doi.org/10.2478/BF02476241

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