Skip to main content

Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection

Central European Journal of Chemistry volume 2, pages 82–90 (2004)Cite this article

Abstract

The paper presents the principles and advantages of a technique combining high performance liquid chromatography and hydride generation atomic absorption spectrometry (HPLC-HGAAS) applied to speciation analysis of inorganic species of arsenic As(III) and As(V) in ground water samples. With separation of the arsenic species on an ion-exchange column in the chromatographic system and their detection by the hydride generation atomic absorption spectrometry, the separation of the analytical signals of the arsenic species was excellent at the limits of determination of 1.5 ng/ml As(III) and 2.2 ng/ml As(V) and RSD of 4.3% and 7.8% for the concentration of 25 ng/ml. The hyphenated technique has been applied for determination of arsenic in polluted ground water in the course of the study on migration of micropollutants. For total arsenic concentration two independent methods: HGICP-OES and HGAAS were used for comparison of results of real samples analysis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. M.A. Suner, V. Devesa, I. Rivas, D. Velez and R.J. Montoro: “Application of column switching in high performance liquid chromatography with on line thermo oxidation and detection by HG AAS and HG AFS for the analysis of organoarsenical species in seafood samples”, J. Anal. At. Spectrom., Vol. 16, (2001), pp. 390–397.

    Article  CAS  Google Scholar 

  2. P. Niedzielski, M. Siepak, J. Przybyłek and J. Siepak: “Arsenic, antimony and selenium in water city of Poznań”, Betagraf, Poznań, 2002, (in Polish).

    Google Scholar 

  3. J.S. Hill, T.A. Arowolo, O.T. Butler, S.R.N. Chenery, J.M. Cook, M.S. Cresser and D.L. Miles: “Atomic spectrometry update. Environmental analysis”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 284–317.

    Article  CAS  Google Scholar 

  4. A. Taylor, S. Branch, D. Halls, M. Patriarca and M. White: “Atomic spectrometry update. Clinical and biological materials, foods and beverages”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 414–455.

    Article  CAS  Google Scholar 

  5. P. Niedzielski, M. Siepak, J. Siepak and J. Przybyłek: “Determination of different forms of arsenic antimony and selenium in water samples using hydride generation”, Polish. Pol. J. Environ. Stud. Vol. 11, (2002), pp. 219–224.

    CAS  Google Scholar 

  6. T. Nakazato, T. Taniguchi, H. Tao, M. Tominaga and A. Miyazaki: “Ion-exclusion chromatography combined with ICP-MS and hybride generation-ICP-MS for the determination of arsenic species in biological matrices”, J. Anal. At. Spectrom., Vol. 15, (2000), pp. 1546–1552.

    Article  CAS  Google Scholar 

  7. Y. Bohari, A. Astruc, M. Astruc and J. Cloud: “Improvements of hybride generation of the speciation of arsenic in natural freshwater samples by HPLC-HG-AFS”, J. Anal. At. Spectrom., Vol. 16, (2001), pp. 774–778.

    Article  CAS  Google Scholar 

  8. B. He, G. Jiang and X. Xu: “Arsenic speciation based on ion exchange high-performance liquid chromatography hyphenated with hydride generation atomic fluorescence and on-line UV photo oxidation”, Fresenius J. Anal. Chem., Vol. 368, (2000), pp. 803–808.

    Article  CAS  Google Scholar 

  9. J.T. Elteren, V. Stibilij and Z. Slejkovec: “Speciation of inorganic arsenic in some bottled Slovene mineral waters using HPLC-HGAFS and selective coprecipitation combined with FI-HGAFS”, Water Research, Vol. 36, (2002), pp. 2967–2974.

    Article  Google Scholar 

  10. D.L. Tsalev, M. Sperling and B. Welz: “Flow-injection hydride generation atomic absorption spectrometric study of the automated on-line pre-reduction of arsenate, methylarsonate and dimethylarsinate and high-performance liquid chromatographic separation of their L-cysteine complexes”, Talanta, Vol. 51, (2000), pp. 1059–1068.

    Article  CAS  Google Scholar 

  11. D.L. Tsalev, M. Sperling and B. Welz: “On-line UV-photooxidation with peroxodisulfate forautomated flow injection and for high-performanceliquid chromatography coupled to hydride generationatomic absorption spectrometry”, Spectrochimica Acta Part B, Vol. 55, (2000), pp. 3390–353.

    Google Scholar 

  12. M.C. Villa-Lojo, E. Alonso-Rodriguez, P. Lopez-Mahia, S. Muniategui-Lorenzo and D. Prada-Rodriguez: “Coupled high performance liquid chromatography-microwave digestion-hydride generation-atomic absorption spectrometry for inorganic and organic arsenic speciation in fish tissue”, Talanta, Vol. 57, (2002), pp. 741–750.

    Article  CAS  Google Scholar 

  13. W.Ch. Tseng, M.H. Yang, T.P. Chen and Y.L. Huang: “Automated, continuous, and dynamic speciation of urinary arsenic in the bladder of living organisms using microdialysis sampling coupled on-line with high performance liquid chromatography and hydride generation atomic absorption spectrometry”, Analyst, Vol. 127, (2002), pp. 560–564.

    Article  CAS  Google Scholar 

  14. Q. Xie, R. Kerrich, E. Irving, K. Liber and F. Abou-Shakra: “Determination of five arsenic species in aqueous samples by HPLC coupled with a hexapole collision cell ICP-MS”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 1037–1041.

    Article  CAS  Google Scholar 

  15. G. Koellensperger, J. Nurmi, S. Hann, G. Stingeder, W.J. Fitz and W.W. Wenzel: “CE-ICP-SFMS and HPIC-ICP-SFMS for arsenic speciation in soil solution and soil water extracts”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 1042–1047.

    Article  CAS  Google Scholar 

  16. P. Niedzielski, J. Siepak and M. Siepak: “Total Content of Arsenic, Antimony and Selenium in Groundwater Samples from Western Poland”, Pol. J. Environ. Stud., Vol. 5, (2001), pp. 347–350.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Water and Soil Analysis, Adam Mickiewicz University, 24 Drzymały Street, 60-613, Poznań, Poland

    P. Niedzielski

  2. Department of Hydrogeology and Waters Protection, Adam Mickiewicz University, 16 Maków Polnych Street, 61-686, Poznań, Poland

    M. Siepak

  3. Laboratory of Atomic Spectrochemistry, Masaryk University, 2 Kotlářská Street, 611 37, Brno, Czech Republic

    K. Novotny

Authors
  1. P. Niedzielski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Siepak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Novotny
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Niedzielski, P., Siepak, M. & Novotny, K. Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection. cent.eur.j.chem. 2, 82–90 (2004). https://doi.org/10.2478/BF02476185

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.2478/BF02476185

Keywords

  • speciation
  • hyphenated techniques
  • arsenic
  • hydride generation absorption atomic spectrometry
  • high performance liquid chromatography