Genetic stability assessment of Taraxacum pieninicum plantlets after long-term slow growth storage using ISSR and SCoT markers
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Genetic stability is highly important in terms of endangered species gene banks. Culture and cold stresses under in vitro conditions may lead to genetic variability. Many methods and possibilities to design appropriate starters for DNA-fingerprinting purposes increase cost and time consumption. Furthermore, multiplicity of possible primers makes it difficult to standardize plant research. The aim of this study was to verify effectiveness of various methods in assessing the clonal homogeneity of Taraxacum pieninicum plantlets regenerated after long-term in vitro cold-storage and to simplify the selection of the genetic stability analysis for Asteraceae family. Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT) polymorphism assays were performed to detect DNA sequence variation. No differences were observed using 16 ISSR markers and 12 SCoT markers between regrown plantlets after storage and plants cultivated from seeds in a soil. Furthermore, SCoT markers were most effective for screening T. pieninicum genome and appeared to be highly useful for different micropropagated and endangered species of Asteraceae family.
KeywordsAsteraceae Cold storage Endangered species Micropropagation Molecular markers
Arbitrarily Amplified Dominant
Amplified Fragment Length Polymorphism
Inter-Retrotransposon Amplified Polymorphism
Inter Simple Sequence Repeats
Methylation Sensitive Amplified Polymorphism
Qualitative Trait Loci
Random Amplified Polymorphic DNA
Retrotransposon-Microsatellite Amplified Polymorphism
Restriction Fragment Length Polymorphism
Start Codon Targeted
Sequence-Specific Amplified Polymorphism
This project was supported by funds from Ministry of Science and Higher Education (PL) for young scientists from Nicolaus Copernicus University in Toruń (2827-B).
Monika Kamińska designed and carried out all the experiments, analyzed the data and wrote the manuscript, Alina Trejgell helped in analysis of the data and in writing the manuscript, and Andrzej Tretyn helped in preparing the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
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