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Nitrogen enriched biochar-compost mixture as a soil amendment to the Haplic Luvisol: effect on greenhouse gas emission

  • Elena Y. Rizhiya
  • Ján HorákEmail author
  • Vladimír Šimanský
  • Natalya P. Buchkina
Original Article


The effect of a nitrogen enriched biochar mixed with compost (FBcCmp) in ratio 50:50 v/v on carbon dioxide (CO2) and nitrous oxide (N2O) emission from Haplic Luvisol was studied in a laboratory experiment. Biochar was produced from grain husks and paper fiber sludge and enriched with 10% ammonium sulfate solution; compost was generated from green waste, milk sludge and stone powder. Eight treatments were used in the experiment: control (Co), biochar (Bc), compost (Cmp), 10% ammonium sulfate solution(F) and their mixtures: Bc + Cmp, Bc + F, Cmp + F, and FBcCmp, which were applied to the soil at two rates equal to10 and 20 t ha−1(rate I and II), respectively. Direct fluxes of CO2 and N2O from the mesocosms were measured using the closed chamber technique. Application of FBcCmpin both rates significantly (P < 0.05) decreased the cumulative CO2 fluxes (by 47–64%)and N2O (by 62–77%) compared tothe other treatments containing inorganic fertilizer (F, Bc + F, Cmp + F) and significantly (P < 0.05) increased concentration of ammonium nitrogen in the soil by 16–21% as compared to treatments with organic fertilizers Cmp and Cmp + Bc. Our findings showed that the application of FBcCmp to the Haplic Luvisol presents environmental benefits to crop production, with the possibility for farmers to put higher concentrations of nutrients into the soil and restrict greenhouse gas emission from the soil.


Ammonium sulfate Biochar Compost Greenhouse gas emissions Nitrogen-enriched biochar mixed with compost 



This study was partly supported by the Slovak Research and Development Agency under the contract [grant number APVV-15-0160] and partly based on the Research plan of the Agrophysical Reseaarh Institute. The authors would like to thank Tomáš Borza and Dr. Elena Aydin for their assistance with the laboratory work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Akiyama H, Tsuruta H, Watanabe T (2000) N2O and NO emissions from soils after the application of different chemical fertilizers. Chemosphere - Glob Change Sci 2:313–320. CrossRefGoogle Scholar
  2. Appel T, Klein B (2015) Mineralization and immobilization of nitrogen in soil amended with biochar, compost and co-composted biochar //in book of abstracts of final meeting EU-COST Action TD 1107 “Biochar” & 76. Symposium Des Anse.V., “Understanding biochar mechanisms for practical implementation”. Hochshule Geisenheim University, 28th–30th September 2015, ISBN 978-3-924618-47-6, pp. 112–113Google Scholar
  3. Asai H, Samson BK, Stephan HM, Songyikhangsuthor K, Homm K, Kiyono Y, Inoue Y, Shiraiwa T, Horie T (2009) Biochar amendment techniques for upland rice production in northern Laos 1. Soil physical properties, leaf SPAD and grain yield. Field Crops Res 111:81–84. CrossRefGoogle Scholar
  4. Bach M, Wilske B, Breuer L (2016) Current economic obstacles to biochar use in agriculture and climate change mitigation. Carbon Manag 7:183–190. CrossRefGoogle Scholar
  5. Bai SH, Blumfield TJ, Reverchon F (2014) The impact of mulch type on soil organic carbon and nitrogen pools in a sloping site. Biol Fertil Soils 50:37–44. CrossRefGoogle Scholar
  6. Buchkina NP, Balashov EV, Rizhiya EY, Smith KA (2010) Nitrous oxide emissions from a light-textured arable soil of North-Western Russia: effects of crops, fertilizers, manures and climate parameters. Nutr Cycl Agroecosyst 87:429–442. CrossRefGoogle Scholar
  7. Buchkina NP, Balashov EV, Šimanský V, Igaz D, Horák J (2017) Changes in biological and physical parameters of soil with different texture after biochar application. Agric Biol 52:471–477. Google Scholar
  8. Buchkina NP, Hüppi R, Leifeld J (2019) Biochar and short-term N2O and CO2 emission from plant residue-amended soil with different fertilisation history. Zemdirbyste-Agriculture 106(2):99–106CrossRefGoogle Scholar
  9. Cayuela ML, van Zwieten L, Singh BP, Jeffery S, Roig A, Sánchez-Monedero MA (2014) Biochar’s role in mitigating soil nitrous oxide emissions: a review and meta- analysis. Agric Ecosyst Environ 191:5–16. CrossRefGoogle Scholar
  10. Chen YX, Huang ZD, Han ZY, Huang Z, Hu B, Shi DZ, Wu WZ (2010) Effect of bamboo charcoal and bamboo vinegar on nitrogen conservation and heavy metals immobility during pig manure composting. Chemosphere 78:1177–1181. CrossRefGoogle Scholar
  11. Chen Y, Yu F, Liang S, Wang Z, Liu Z, Xiong Y (2014) Utilization of solar energy in sewage sludge composting: Fertilizer effect and application. Waste Management 34:2014–2021.
  12. Clough T, Condron L, Kammann C, Müller C (2013) A review of biochar and soil nitrogen dynamics. Agronomy 3:275–293. CrossRefGoogle Scholar
  13. Cornelissen G, Rutherford D, Arp HPH, Doersch P, Kelly CN, Rostad CE (2013) Sorption of pure N2O to biochars and other organic and inorganic materials under anhydrous conditions. Environ Sci Technol 47:7704–7712.
  14. Darby I, Xu CY, Wallace HM, Joseph S, Pace B, Bai SH (2016) Short-term dynamics of carbon and nitrogen using compost, compost biochar mixture and organo-mineral biochar. Environ Sci Pollut Res 23:11267–11278. CrossRefGoogle Scholar
  15. Dempster DN, Jones DL, Murphy DV (2012) Organic nitrogen mineralization in two contrasting agro-ecosystems is unchanged by biochar addition. Soil Biol Biochem 48:47–50. CrossRefGoogle Scholar
  16. Dias BO, Silva CA, Higashikawa FS, Roig A, Sanchez-Monedero MA (2010) Use of biochar as bulking agent for the composting of poultry manure: effect on organic matter degradation and humification. Bioresour Technol 101:1239–1246. CrossRefGoogle Scholar
  17. Gaskin JW, Speir RA, Harris K, Das KC, Lee RD, Morris LA, Fisher DS (2010) Effect of peanut hull and pine chip biochar on soil nutrients, corn nutrient status, and yield. Agronomy Journal 102:623–633.
  18. Glaser B, Birk J (2012) State of the scientific knowledge on properties and genesis of anthropogenic dark earths in Central Amazonia (terra preta de Índio). Geochim Cosmochim Acta 82:39–51. CrossRefGoogle Scholar
  19. Grossman JM, O’neill BE, Tsai SM, Liang B, Neves E, Lehmann J (2010) Amazonian anthrosols support similar microbial communities that differ distinctly from those extant in adjacent, unmodified soils of the same mineralogy. Microb Ecol 60:192–205. CrossRefGoogle Scholar
  20. Horák J (2015) Testing biochar as a possible way to ameliorate slightly acidic soil at the research field located in the Danubian lowland. Acta Horticulturae et Regiotecturae 18:20–24. CrossRefGoogle Scholar
  21. Horák J, Igaz D, Kondrlová E (2014) Short-term soil carbon dioxide (CO2) emission after application of conventional and reduced tillage for red clover in Western Slovakia. Eurasian J Soil Sci 3:206–211. CrossRefGoogle Scholar
  22. Houghton JT, Ding Y, Griggs DJ, Noguer M, van der Linden PJ, Xiaosu D (2001) Climate change 2001: the scientific basis: contributions of working group I to the third assessment report of the intergovernmental panel on climate change. Cambridge University Press, p 881Google Scholar
  23. Igaz D, Šimanský V, Horák J, Kondrlová E, Domanová J, Rodný M, Buchkina NP (2018) Can a single dose of biochar affect selected soil physical and chemical characteristics? J Hydrol Hydromech 66:421–428. CrossRefGoogle Scholar
  24. IUSS Working Group WRB (2014) World reference base for soil resources 2014. World soil resources reports no. 103. FAO, RomeGoogle Scholar
  25. Jones D, Rousk J, Edwards-Jones G, DeLuca TH, Murphy DV (2012) Biochar-mediated changes in soil quality and plant growth in a three year field trial. Soil Biol Biochem 45:113–124. CrossRefGoogle Scholar
  26. Kammann CI, Linsel S, Gößling JW, Koyro HW (2011) Influence of biochar on drought tolerance of Chenopodium quinoa Willd and on soil-plant relations. Plant Soil 345:195–210. CrossRefGoogle Scholar
  27. Karer J, Wimmer B, Zehetner F, Kloss S, Soja G (2013) Biochar application to temperate soils: effects on nutrient uptake and crop yield under field conditions. Agric Food Sci 22:390–403. CrossRefGoogle Scholar
  28. Kithome M, Paul JW, Bomke AA (1999) Reducing nitrogen losses during simulated composting of poultry manure using absorbents or chemical amendments. J Environ Qual 28:194–201. CrossRefGoogle Scholar
  29. Kondrlová E, Horák J, Igaz D, Dobiašová D (2017) The possibility of using digital images in assessment of plant canopy development and weed spread. Acta Horticulturae et Regiotecturae 20:20–24. CrossRefGoogle Scholar
  30. Lehmann J (2007) Bio-energy in the black. Front Ecol Environ 5:381–387.[381:BITB]2.0.CO;2 CrossRefGoogle Scholar
  31. Lehmann J, Rilling MC, Thies J, Massiello CA, Hockaday WC, Crowley D (2011) Biochar effects of soil biota – a review. Soil Biol Biochem 43:1812–1836. CrossRefGoogle Scholar
  32. Lehoczky É, Kamuti M, Mazsu N, Sándor R (2016) Changes to soil water content and biomass yield under combined maize and maize-weed vegetation with different fertilization treatments in loam soil. J Hydrol Hydromech 64:150–159. CrossRefGoogle Scholar
  33. Major J, Rondon M, Molina D, Riha SJ, Lehmann J (2012) Nutrient leaching in a Colombian savanna Oxisol amended with biochar. J Environ Qual 41:1076–1086. CrossRefGoogle Scholar
  34. Mukherjee A, Lal R (2013) Biochar impacts on soil physical properties and greenhouse gas emissions. Agronomy 3:313–339 CrossRefGoogle Scholar
  35. Nelissen V, Rütting T, Huygens D, Staelens J, Ruysschaert G, Boeckx P ( 2012) Maize biochars accelerate short‐term soil nitrogen dynamics in a loamy sand soil. Soil Biology & Biochemistry 55:20–24.
  36. Nielsen S, Minchin T, Kimber S, Van Zwieten L, Gilbert J, Munroe P, Joseph S, Thomas T (2014) Comparative analysis of the microbial communities in agricultural soil amended with enhanced biochars or traditional fertilisers. Agric Ecosyst Environ 191:73–82 CrossRefGoogle Scholar
  37. Parkin TB, Venterea RT, Hargreaves SK (2012) Calculating the detection limits of chamber-based soil greenhouse gas flux measurements. J Environ Qual 41:705–715. CrossRefGoogle Scholar
  38. Prost K, Borchard N, Siemens J, Kautz T, Sequaris JM, Moller A, Amelung W (2013) Biochar affected by composting with farmyard manure. J Environ Qual 42:164–172. CrossRefGoogle Scholar
  39. Quin PR, Cowie AL, Flavel RJ, Macdonald LM, Morris SG, Singh BP, Young IM, Van Zwieten L (2014) Oil mallee biochar improves soil structural properties – a study with X-ray micro-CT. Agric Ecosyst Environ 191:142–149 CrossRefGoogle Scholar
  40. R Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
  41. Rajkovich S, Enders A, Hanley K, Hyland C, Zimmerman AR, Lehmann J (2012) Corn growth and nitrogen nutrition after additions of biochars with varying properties to a temperate soil. Biol Fertil Soils 48: 271–284.
  42. Reverchon F, Flicker RC, Yang H, Yan G, Xu Z, Chen C, Bai SH, Zhang D (2014) Changes in δ15N in a soil–plant system under different biochar feedstocks and application rates. Biol Fertil Soils 50:275–283. CrossRefGoogle Scholar
  43. Schulz H, Glaser B (2012) Effects of biochar compared to organic and inorganic fertilizers on soil quality and plant growth in a greenhouse experiment. J Soil Sci Plant Nutr 175:410–422 CrossRefGoogle Scholar
  44. Schulz H, Dunst G, Glaser B (2014) No effect level of co-composted biochar on plant growth and soil properties in a greenhouse experiment. Agronomy 4:34–51. CrossRefGoogle Scholar
  45. Signor D, Cerri CEP, Conant R (2013) N2O emissions due to nitrogen fertilizer applications in two regions of sugarcane cultivation in Brazil. Environ Res Lett 8:015013.
  46. Šimanský V, Horák J, Igaz D, Jonczak J, Markiewicz M, Felber R, Rizhiya EY, Lukac M (2016) How dose of biochar and biochar with nitrogen can improve the parameters of soil organic matter and soil structure? Biologia 71:989–995. Google Scholar
  47. Šimanský V, Igaz D, Horák J, Šurda P, Kolenčík M, Buchkina NP, Uzarowicz L, Juriga M, Šrank D, Pauková Ž (2018) Response of soil organic carbon and water-stable aggregates to different biochar treatments including nitrogen fertilization. J Hydrol Hydromech 66:429–436. CrossRefGoogle Scholar
  48. Singh BP, Cowie AL (2014) Long-term influence of biochar on native organic carbon mineralisation in a low-carbon clayey soil. Sci Rep 4:1–9. Google Scholar
  49. Spokas KA, Novak JM, Venterea RT (2012) Biochars role as an alternative N-fertilizer: ammonia capture. Plant Soil 350:35–42. CrossRefGoogle Scholar
  50. StatSoft, Inc. Statistica (2001) Data Analysis Software System, Version 6.
  51. Steinbeiss S, Gleixner G, Antonietti M (2009) Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biol Biochem 41:1301–1310. CrossRefGoogle Scholar
  52. Steiner C, Melear N, Harris K, Das KC (2011) Biochar as bulking agent for poultry litter composting. Carbon Manag 2:227–230. CrossRefGoogle Scholar
  53. Taghizadeh-Toosi A, Clough TJ, Condron LM, Sherlock RR, Anderson CR, Craigie RA (2011) Biochar incorporation into pasture soil suppresses in situ nitrous oxide emissions from ruminant urine patches. J Environ Qual 40:468–476. CrossRefGoogle Scholar
  54. Theeba M, Bachmann RT, Illani ZI, Zulkefli M, Husni MHA, Samsuri AW (2012) Characterization of local mill rice husk charcoal and its effect on compost properties. Malays J Soil Sci 16:89–102 Google Scholar
  55. Ulyett J, Sakrabani R, Kibblewhite M, Hann M (2014) Impact of biochar addition on water retention, nitrification and carbon dioxide evolution from two sandy loam soils. Eur J Soil Sci 65:96–104. CrossRefGoogle Scholar
  56. Uzoma KC, Inoue M, Andry H, Zahoor A, Nishihara E (2011) Influence of biochar application on sandy soil hydraulic properties and nutrient retention. J Food Agric Environ 9:1137–1143. Google Scholar
  57. Van Zwieten L, Singh BP, Kimber SWL, Murphy DV, Macdonald LM, Rust J, Morris S (2014) An incubation study investigating the mechanisms that impact N2O flux from soil following biochar application. Agri Ecosys Environ 191:53–62. CrossRefGoogle Scholar
  58. Wang Y, Pan F, Wang G, Zhang G, Wang Y, Chen X, Mao Z (2014) Effects of biochar on photosynthesis and antioxidative system of Malus hupehensis Rehd. seedlings under replant conditions. Sci Hortic 175:9–15. CrossRefGoogle Scholar
  59. Xu CY, Bai SH, Hao Y, Rachaputi RN, Wang H, Xu Z, Wallace H (2015) Effect of biochar amendment on yield and photosynthesis of peanut on two types of soils. Environ Sci Pollut Res 22:6112–6125. CrossRefGoogle Scholar

Copyright information

© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of Soil BiophysicsAgrophysical Research InstituteSt. PetersburgRussia
  2. 2.Department of Biometeorology and Hydrology, Horticulture and Landscape Engineering FacultySlovak University of Agriculture in NitraNitraSlovakia
  3. 3.Department of Soil Science, Faculty of Agrobiology and Food ResourcesSlovak University of Agriculture in NitraNitraSlovakia

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