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Biologia

, Volume 74, Issue 12, pp 1675–1686 | Cite as

Presence/absence of a CACTA transposon in the CYC2c gene of two genotypes of Helianthus × multiflorus cv. “Meteor” characterized by a radiate inflorescence with different shape of disk flower corollas

  • Marco Fambrini
  • Claudio PugliesiEmail author
Original Article
  • 25 Downloads

Abstract

Helianthus × multiflorus is a triploid (2n = 51) interspecific hybrid derived from the cross of H. decapetalus with H. annuus that exists in radiate and ligulate inflorescence types. The H. × multiflorus “Soleil d’Or” has a ligulate inflorescence, with actinomorphic corolla of disk flowers converted in zygomorphic ray-like corollas. In “Soleil d’Or”, a truncated CACTA transposable element (TE), named CTEHM1, is integrated in the HmCYC2c gene, a key player controlling zygomorphism in the Helianthus genus. Here, we showed that H. × multiflorus “Meteor”, originated from a mutation of “Soleil d’Or”, exists in two different types of radiate inflorescence, here named “Meteor 1” and “Meteor 2”. Notably, in “Meteor 1”, which displayed a corolla of disk flowers typical for the Helianthus genus, CTEHM1 was transposed from the HmCYC2c gene. In contrast, the CTEHM1 TE was still present in the HmCYC2c gene of “Meteor 2”. In this genotype, the largest corolla of the disk flowers shows some features of the ray flowers giving rise to a radiate inflorescence unusual for the Helianthus genus. The in silico analysis of CTEHM1 also highlights the presence of a 202 bp CpNpG island, which is a putative target of methylation. Interestingly, the gene expression of the HmCYC2c gene was significantly higher in disk flowers of “Meteor 2” compared to “Meteor 1”. Stated these results, we suggest that both the excision of a TE and an epigenetic regulation of gene expression generate the two-inflorescence types of H. × multiflorus, “Meteor 1” and “Meteor 2”.

Keywords

CACTA transposable elements Carotenoids Flower symmetry Helianthus × multiflorus Inflorescence architecture 

Abbreviations

CIN

CINCINNATA

CDS

Coding sequence

CYC

CYCLOIDEA

DF

Disk flowers

bHLH

basic Helix-Loop-Helix

PCF

PROLIFERATING CELL FACTOR

RF

Ray flowers

RT-qPCR

Real-Time quantitative PCR

TCP

TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTOR

TB1

Teosinte Branched1

TF

Transcription factor

TSS

Transcription Start Site

TE

Transposable element

CTEHM1

CACTA Transposable Element of Helianthus × multiflorus1

3-, 5’-UTR

3′-, 5′-untranslated region

Notes

Acknowledgments

We are indebted to Mariangela Salvini and Gabriele Usai for assistance in bioinformatics analyses.

Funding

The Special Fund 2017–2018 of the University of Pisa funded this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with ethical standards

Conflict of interest

Marco Fambrini declares that he has no conflict of interest. Claudio Pugliesi declares that he has no conflict of interest.

Supplementary material

11756_2019_301_MOESM1_ESM.pdf (176 kb)
ESM 1 (PDF 176 kb)
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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

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