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Biologia

, Volume 74, Issue 8, pp 1045–1053 | Cite as

Physiological and biochemical responses of Dunaliella salina exposed to acrylamide

  • Na LingEmail author
  • Hong-Xiu Li
  • Hong-Shi Guo
  • Xiu-Ming Cao
  • Xiao-Rui Liu
Original Article
  • 25 Downloads

Abstract

In the present study, physiological and biochemical responses of Dunaliella salina exposed to acrylamide were investigated. The indicators were as follows: the growth, photosynthetic pigments, the contents of carbohydrate and protein, antioxidant system of Dunaliella salina. Besides, the ultrastructure and cell cycle distribution were also analyzed. The results showed that acrylamide could significantly inhibit the growth of D. salina (P < 0.05 or P < 0.01), and reduce the contents of photosynthetic pigments, carbohydrate and protein in D. salina cells. In addition, according to transmission electron microcopy (TEM) and flow cytometry (FCM), acrylamide could change cell ultrastructure and induce G1 phase arrest in D. salina cells. With the increase of acrylamide concentrations, the activities of superoxide dismutase (SOD) and catalase (CAT) in D. salina showed a rise first followed by a decline, whereas malondialdehyde (MDA) content increased sharply (P < 0.05 or P < 0.01). The results suggested that acrylamide was slightly toxic to Dunaliella salina and cause the physiological and biochemical changes. Anti-oxidation and cell cycle arrest may be the defense mechanism of algae responding to acrylamide.

Keywords

Acrylamide Dunaliella salina Physiological & biochemical responses Antioxidant system Ultrastructure Cell cycle 

Abbreviations

AM

Acrylamide

PAMs

Polyacrylamides

TU

Toxicity unit

ROS

Reactive oxygen species

SOD

Superoxide dismutase

CAT

Catalase

GPx

Glutathione peroxidase

MDA

Malondialdehyde

IR

Inhibitory rate

EC50

Median effective concentration

TEM

Transmission electron microcopy

FCM

Flow cytometry

PI

Propidium iodide

SD

Standard deviation

HSPs

Heat stock proteins

PCD

Programmed cell death.

Notes

Acknowledgments

This work was supported by the Natural Science Foundation of HeiLongJiang (No. C2018037), (No. C201123).

Compliance with ethical standards

Conflict of interest

The authors declared no conflict of interest.

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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Zhejiang Shuren UniversityHangzhouPeople’s Republic of China
  2. 2.College of ScienceHarbin University of CommerceHarbinPeople’s Republic of China

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