Application of the alizarin red S mass marking technique and its detection in stocked asp (Leuciscus aspius)
- 16 Downloads
Alizarin Red S marking and detection was tested on asp (Leuciscus aspius Linnaeus, 1758) in an experimental pool followed by a mark-recapture experiment in the field. Placing the asp in a 0.15 g L−1 solution for one hour produced marks that were detectable for at least one year. In order to detect the fluorescent marks, novel equipment was developed comprising a battery-powered laser source (<100 mW laser pointer, wavelength 532 nm) and protective filtration goggles (filtering wavelength 190–540 nm). The detection equipment and procedure proved satisfactory for marking large numbers of fish and for detection in the field. It allowed repeat observations of the same fish and examination of fin rays (eventually fin clips), which are usually too large to examine under a standard microscope. This method is highly recommended for future studies.
KeywordsAspius aspius Fin rays Fluorescence Green laser Non-invasive detection
This study was supported through project QJ1620240 “Application of “top-down” biomanipulation to reduce eutrophication caused by agriculture in reservoirs“. Thanks are given to Dr. Kevin Roche for language correction.
Compliance with ethical standards
All applicable international, national and institutional guidelines for the care and use of animals were followed.
Conflict of interest
The authors declare that no competing interests exist.
- Fielder DG (2002) Methodology for immersion marking walleye fry and fingerlings in oxytetracycline hydrochloride and its detection with fluorescence microscopy. Fish Tech Rep 2002-01 Michigan Dept of Natural Resources, Fisheries DivisionGoogle Scholar
- Leips J, Baril CT, Rood FH, Reznick DN, Bashey F (2001) The suitability of calcein to mark poeciliid fish and a new method of detection. Trans Am Fish Soc 130:501–207. https://doi.org/10.1577/1548-8659(2001)130<0501:TSOCTM>2.0.CO;2 CrossRefGoogle Scholar
- Lü HJ, Fu M, Xi D, Yao WZ, Su SQ, Wu ZL (2015a) Experimental evaluation of using calcein and alizarin red S for immersion marking of bighead carp Aristichthys nobilis (Richardson, 1845) to assess growth and identification of marks in otoliths, scales and fin rays. J Appl Ichthyol 31:665–674. https://doi.org/10.1111/jai.12778 CrossRefGoogle Scholar
- Martyniak A, Stańczak K, Kozłowski J, Mierzejewska K, Wziątek B, Lejk AM, Hliwa P (2013) Alizarin mark retention in the otoliths of whitefish (Coregonus lavaretus f. lavaretus L.) from Lake Łebsko, Poland. Advanc Limnol 64:83–89. https://doi.org/10.1127/1612-166X/2013/0064-0009 CrossRefGoogle Scholar
- Nielsen LA (1992) Methods of marking fish and shellfish, Special publication. American Fisheries Society. Bethesda, MarylandGoogle Scholar
- Rutherford ES, Iacono JD, Callaham G (2002) Evaluation of marking procedures to estimate natural reproduction of Chinook salmon in Lake Michigan. Great Lakes Fishery Cimmission, Project Completion ReportGoogle Scholar
- Skov C, Gronkjaer P, Nielse C (2001) Marking pike fry otoliths with alizarin complexone and strontium: an evaluation of methods. J Fish Biol 59:745–750. https://doi.org/10.1111/j.1095-8649.2001.tb02377.x CrossRefGoogle Scholar
- Skov C, Koed A, Baastrup-Spohr L, Arlinghaus R (2011) Dispersal, growth, and diet of stocked and wild northern pike fry in a shallow natural lake, with implications for the management of stocking programs. North Am J Fish Manag 31:1177–1186. https://doi.org/10.1080/02755947.2011.646452 CrossRefGoogle Scholar
- Ward DL (2003) Effects of marking techniques and handling on swimming ability of bonytail chub. J Ariz Nev Acad Sci 36(1):34–36Google Scholar