Enhancement of capsaicinoids in vitro production by abiotic elicitors in placenta-derived callus of Capsicum annuum L. Tunisian var. ‘Baklouti Medenine’
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Production of natural phytochemicals via tissue culture is an efficient method for both laboratory and industrial-scale production. To evaluate the influence of abiotic elicitation on in vitro production of capsaicinoids (CAPs), callogenesis was induced from the placenta of pepper (Capsicum annuum L.) Tunisian var. ‘Baklouti Medenine’ on MS medium supplemented with 9.04 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 μM kinetin (Kin). Placenta-derived callus was subjected to different concentrations of sucrose, sodium chloride (NaCl), polyethylene glycol (PEG 6000), and silver nitrate (AgNO3). After 4 wk. of culture, callus was harvested and capsaicin (C) and dihydrocapsaicin (DHC) were quantified by liquid chromatography-mass spectrometry (LC-MS). Maximum callus production was obtained with 34.22 mM and 68.44 mM NaCl, with an improvement in growth index (GI) by 3481% and 3736% respectively. In elicitation-free culture, DHC was the major CAPs (79.27%). The highest accumulation of DHC was obtained with 17.11 mM NaCl (39.52 mg g−1 DW; 4% of callus DW). Meanwhile, elicitation raised C content to approximately three times that of DHC. C production increased from 0.17 mg g−1 DW in control culture to 27.88 and 26.79 mg g−1 DW at 17.11 mM NaCl and 14.71 μM AgNO3, respectively. For both treatments, C represented more than 2.6% of callus DW. 17.11 mM NaCl-treated callus, which produced the highest concentrations of both C and DHC, showed the least callus production. Hence, Tunisian var. ‘Baklouti Medenine’ appears an interesting resource for these alkaloids biosynthesis and can be used to develop a large scale production of valuable CAPs compounds.
KeywordsCapsicum Capsaicinoids In vitro Osmotic elicitation Silver nitrate
This research was not supported by any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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Conflicts of interest
The authors declare that they have no conflict of interest in the publication.
- Ben Mansour-Gueddes S (2015) Evolution of capsaicinoids and mineral composition during fruit development in some hot pepper varieties (Capsicum annuumL.). Int J Agric Innov Res 3:1094–1102Google Scholar
- Cheema SK, Pant MR (2011) Estimation of capsaicin in seven cultivated varieties of Capsicum annuum L. res J pharm biol Chem Sci 2:701–706 ISSN: 0975-8585Google Scholar
- González-Zamora A, Sierra-Campos E, Luna-Ortega JG, Pérez-Morales R, Ortiz JCR, García-Hernández JL (2013) Characterization of different Capsicum varieties by evaluation of their capsaicinoids content by high performance liquid chromatography. Determination of pungency and effect of high temperature. Molecules 18:13471–13486. https://doi.org/10.3390/molecules181113471 CrossRefGoogle Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
- Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182–206. https://doi.org/10.3390/molecules21020182 CrossRefGoogle Scholar
- Ruiz-Lau N, Medina-Lara F, Minero-Garcıa Y, Zamudio-Moreno E, Guzman-Antonio A, Echevarrıa-Machado I, Martınez-Estevez M (2011) Water deficit affects the accumulation of capsaicinoids in fruits of Capsicum chinense Jacq. Hortscience 46:487–492. https://doi.org/10.21273/HORTSCI.46.3.487 CrossRefGoogle Scholar
- Sahraroo A, Babalar M, Mirjalili MH, Fattahi Moghadam MR, Nejad Ebrahimi S (2014) In vitro callus induction and rosmarinic acid quantification in callus culture of Satureja khuzistanica Jamzad (Lamiaceae). Iran J Pharm Res 13:1447–1456Google Scholar
- Seigler DS (1998) Plant secondary metabolism. Boston: Chapman and Hall (Kluwer Academic Publishers): 711Google Scholar
- Sudha G, Ravishankar GA (2003a) Influence of methyl jasmonate and salicylic acid in the enhancement of capsaicin production in cell suspension cultures of Capsicum frutescens Mill Curr Sci 85:1212–1217. https://www.jstor.org/stable/24108623. Accessed 21 March 2019
- Sung Y, Chang YY, Ting NL (2005) Capsaicin biosynthesis in water-stressed hot pepper fruits. Bot Bull Acad Sin 46:35–42Google Scholar
- Weiss EA (2002) Spice Crops. CABI Publishing International, New York, p 411. https://trove.nla.gov.au/version/40049661