Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)
- 59 Downloads
Inhibition of the proliferation of human tumour cells and porcine non-tumour cells by the pygidial gland secretion released by adults of four ground beetle species was observed in this study. The sulphorhodamine B (SRB) assay was applied to establish the percentages of inhibition of the net growth of four human tumour cell lines and porcine liver primary non-tumour cells. The secretions of all tested ground beetle species were shown to have an antiproliferative effect on the tested cell lines. Special emphasis is put on the secretion of Abax parallelepipedus, which showed the highest antitumour potential and weakest inhibition of non-tumour cell proliferation. The antitumour and antiproliferative potential of the pygidial gland secretions of ground beetles is here demonstrated for the first time. It is suggested that certain organic acids are responsible for the action. Further investigation needs to be conducted in order to better understand the mechanisms governing the observed cytotoxic and antitumour activity.
KeywordsPygidial gland secretions Ground beetles Antiproliferative effect Cell lines Ellipticine
The study was financially supported by the Serbian Ministry of Education, Science, and Technological Development (Grants Nos. 173038 and 173032) and the Foundation for Science and Technology (FCT, Portugal) (Grant No. SFRH/BPD/68344/2010).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- Calhelha RC, Ferreira ICFR, Peixoto D, Abreu RMV, Vale-Silva LA, Pinto E, Lima RT, Alvelos MI, Vasconcelos MH, Queiroz MJ (2012) Aminodi(hetero)arylamines in the thieno[3,2-b]pyridine series: synthesis, effects in human tumor cells growth, cell cycle analysis, apoptosis and evaluation of toxicity using non-tumor cells. Molecules 17:3834–3843. https://doi.org/10.3390/molecules17043834 CrossRefPubMedGoogle Scholar
- Dazzini-Valcurone M, Pavan M (1980) Glandole pigidiali e secrezioni difensive dei Carabidae (Insecta Coleoptera). Publ Ist Entomol Pavia 12:1–36Google Scholar
- Dettner K (1987) Chemosystematics and evolution of beetle chemical defenses. Annu Rev Entomol 32:17–48. https://doi.org/10.1146/annurev.en.32.010187.000313 CrossRefGoogle Scholar
- Entin-Meer M, Rephaeli A, Yang X, Nudelman A, VandenBerg SR, Haas-Kogan DA (2005) Butyric acid prodrugs are histone deacetylase inhibitors that show antineoplastic activity and radiosensitizing capacity in the treatment of malignant gliomas. Mol Cancer Ther 4:1952–1961. https://doi.org/10.1158/1535-7163.MCT-05-0087 CrossRefPubMedGoogle Scholar
- Ghaoneim K (2014) Cantharidin as promising chemotherapeutic agent in the modern medicine: a comprehensive review. J Sci 4:272–292Google Scholar
- Kurata S, Morishita K, Kawase T, Umemoto K (2012) Cytotoxic effects of acrylic acid, methacrylic acid, their corresponding saturated carboxylic acids, HEMA, and hydroquinone on fibroblasts derived from human pulp. Dent Mater J 31:219–225. https://doi.org/10.4012/dmj.2011-085 CrossRefPubMedGoogle Scholar
- Moore BP (1979) Chemical defense in carabids and its bearing on phylogeny. In: Erwin TL, Ball GE, Whitehead DR, Halpern AL (eds) Carabid beetles: their evolution, natural history, and classification. Dr. W. Junk bv Publishers, Dordrecht, pp 193–203. https://doi.org/10.1007/978-94-009-9628-1_9
- Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić-Mataruga V, Ilijin L, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S (2016a) Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). Sci Nat 103:34. https://doi.org/10.1007/s00114-016-1358-z CrossRefGoogle Scholar
- Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić-Mataruga V, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S (2016b) Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). Bull Entomol Res 106:474–480. https://doi.org/10.1007/s00253-016-8082-7 CrossRefPubMedGoogle Scholar
- Nenadić M, Ljaljević-Grbić M, Stupar M, Vukojević J, Ćirić A, Tešević V, Vujisić L, Todosijević M, Vesović N, Živković N, Ćurčić S (2017) Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes. Sci Nat 104:52. https://doi.org/10.1007/s00114-017-1474-4
- Paoletti C, Le Pecq JB, Dat-Xuong N, Juret P, Garnier H, Amiel JL, Rouesse J (1980) Antitumor activity, pharmacology, and toxicity of ellipticines, ellipticinium, and 9-hydroxy derivatives: preliminary clinical trials of 2-methyl-9-hydroxy ellipticinium (NSC 264-137). In: Mathé G, Muggia FM (eds) Cancer chemo- and immunopharmacology. Recent Results Cancer Res, vol 74. Springer Verlag, Berlin-Heidelberg, pp 107–123. https://doi.org/10.1007/978-3-642-81488-4_15 CrossRefGoogle Scholar
- Vesović N, Ćurčić S, Vujisić L, Nenadić M, Krstić G, Perić-Mataruga V, Milosavljević S, Antić D, Mandić B, Petković M, Vučković I, Marković Đ, Vrbica M, Ćurčić B, Makarov S (2015) Molecular diversity of compounds from pygidial gland secretions of cave-dwelling ground beetles (Insecta: Coleoptera: Carabidae): the first evidence. J Chem Ecol 41:533–539. https://doi.org/10.1007/s10886-015-0593-7 CrossRefPubMedGoogle Scholar
- Vesović N, Vujisić L, Perić-Mataruga V, Krstić G, Nenadić M, Cvetković M, Ilijin L, Stanković J, Ćurčić S (2017) Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus (Tomocarabus) convexus and C. (Procrustes) coriaceus (Coleoptera: Carabidae). J Nat Hist 51:545–560. https://doi.org/10.1080/00222933.2017.1293183 CrossRefGoogle Scholar