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Biologia

, Volume 73, Issue 4, pp 425–435 | Cite as

Genome-wide identification and characterization of cysteine-rich polycomb-like protein (CPP) family genes in cucumber (Cucumis sativus) and their roles in stress responses

  • Yong Zhou
  • Lifang Hu
  • Shuifeng Ye
  • Lunwei Jiang
  • Shiqiang Liu
Original Article
  • 41 Downloads

Abstract

Cysteine-rich polycomb-like protein (CPP) transcription factors are a small gene family involved in the regulation of plant growth, development and stress response. Genome-wide identification and characterization of CPP gene family have been carried out in several plant species. However, no comprehensive phylogenetic analysis or expression profiling of CPP genes in Cucumis sativus has been reported. In this study, we characterized the CPP family genes in C. sativus, and 5 CPP candidate genes were found in cucumber genome. CPP proteins from cucumber and other plant species were classified into two groups, which were further divided into five subgroups based on the phylogenetic distribution. Most CPP genes in the same subgroup shared similar gene structures and conserved motifs. Transcriptome data revealed that CsCPP genes were expressed in leaves, ovaries, flowers, stems, roots, and tendril tissues. qRT-PCR expression analysis showed that many CsCPP genes exhibited different expression patterns in cucumber leaves under abiotic stresses including salt, cold, drought, and ABA. These results demonstrate that cucumber CPP gene family may play critical roles in plant development as well as in the responses to environmental stresses.

Keywords

Cucumis sativus Cysteine-rich polycomb-like protein (CPP) Gene family Expression analysis Abiotic stress 

Abbreviations

ABA

Abscisic acid

tso

Chinese for ‘ugly’

CDS

Coding sequence

CHC

CXC-Hinge-CXC

CPP

Cysteine-rich polycomb-like protein

CRC

C1-RNP(I/L/N)AFAPK-C2

FPKM

Fragments per kb per million mapped read

gDNA

Genomic DNA

GRAVY

Grand average of hydropathy index

HMM

Hidden Markov model

pI

Isoelectric point

MW

Molecular weight

TCX

Tesmin/TSO1-like CXC

TF

Transcription factors

TCR

TSO1 Cysteine-Rich

qRT-PCR

Quantitative real-time PCR

Notes

Acknowledgments

This work was funded by the Key Project of Youth Science Foundation of Jiangxi Province (20171ACB21025), and the National Natural Science Foundation of China (31460522 and 31660578).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11756_2018_49_MOESM1_ESM.doc (46 kb)
Table S1 (DOC 46 kb)
11756_2018_49_MOESM2_ESM.doc (134 kb)
Table S2 (DOC 134 kb)

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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2018

Authors and Affiliations

  1. 1.College of ScienceJiangxi Agricultural UniversityNanchangChina
  2. 2.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of EducationJiangxi Agricultural UniversityNanchangChina
  3. 3.Shanghai Agrobiological Gene CenterShanghaiChina

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