, Volume 68, Issue 5, pp 1004–1009

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Section Cellular and Molecular Biology

DOI: 10.2478/s11756-013-0230-2

Cite this article as:
Aydin, E., Türkez, H. & Geyikoğlu, F. Biologia (2013) 68: 1004. doi:10.2478/s11756-013-0230-2


α-Pinene, an organic monoterpene, is found in essential oils of pine and coniferous trees. To date, although various biological activities of α-pinene have been demonstrated, its neurotoxicity has never been explored. Therefore in this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, genotoxic damage potentials by single cell gel electrophoresis, and oxidative effects by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis of α-pinene. Statistical analysis of MTT assay results indicated significant (p < 0.05) decreases of the cell proliferation rates in healthy neurons treated with α-pinene at only 400 mg/L, while significant decreases were observed in N2a cells at 100, 200 and 400 mg/L. On the other hand, the mean values of the total scores of cells showing DNA damage were not found significantly different from the control values on both cells. In addition, our results indicated that 10 and 25 mg/L of α-pinene treatment caused increases of TAC levels in primary rat neurons without any alterations of its level in N2a cells. However, α-pinene treatments at higher doses led to increases of TOS levels in both cell types. Overall our results suggest that α-pinene is of a limited therapeutic use as an anticancer agent.

Key words

alpha-pinene comet assay MTT assay neurotoxicity N2a neuroblastoma cell line oxidative status 



3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide




single cell gel electrophoresis


total antioxidant capacity


total oxidative stress

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  • Elanur Aydin
    • 1
  • Hasan Türkez
    • 2
  • Fatime Geyikoğlu
    • 1
  1. 1.Department of Biology, Faculty of ScienceAtatürk UniversityErzurumTurkey
  2. 2.Department of Molecular Biology and Genetics, Faculty of ScienceErzurum Technical UniversityErzurumTurkey

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