Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp. ALSHL3
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An Indonesian marine bacterial isolate, which belongs to genus of Bacillus sp. based on 16S rDNA analysis and was identified as Bacillus filicolonicus according to its morphology and physiology, produced a raw starch degrading α-amylase. The partially purified α-amylase using a maize starch affinity method exhibited an optimum pH and temperature of 6.0 and 60°C, respectively. The enzyme retained 72% of its activity in the presence of 1.5 M NaCl. Scanning electron micrographs showed that the α-amylase was capable of degrading starch granules of rice and maize. This α-amylase from Bacillus sp. ALSHL3 was classified as a saccharifying enzyme since its major final degradation product was glucose, maltose, and maltotriose.
Key wordsα-amylase Bacillus filicolonicus raw starch maize marine
- Buchanan R.E., Gibbons N.E., Cowan S.T., Holt J.G., Liston J., Muray R.G.E., Niven C.F., Ravin A.W. & Stanier R.W. 1974. Bergey’s Manual of Determinative Bacteriology, 8th Edition. The Williams and Wilkins Company, Baltimore.Google Scholar
- Fuwa H. 1954. A new method for microdetermination of amylase activity by the use of amylose as a substrate. J. Biochem. 21: 219–230.Google Scholar
- Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426 428.Google Scholar
- Robyt J.F. 1998. Essentials of Carbohydrate Chemistry. Springer, Boston, 399 pp.Google Scholar