Chemical Papers

, Volume 64, Issue 6, pp 729–733 | Cite as

Determination of catechin and epicatechin in the peel of apple varieties resistant and non-resistant to apple scab

  • Marek Gołębiowski
  • Edmund Maliński
  • Małgorzata Szankin
  • Mariusz Marszeniuk
  • Monika Paszkiewicz
  • Piotr Stepnowski
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Catechin and epicatechin were analysed in the peel of six apple cultivars (three resistant and three non-resistant to apple scab). Two methods of analytical sample preparation following extraction were tested: solid phase extraction and column separation with Sephadex LH-20 coupled to a differential refractometric detector. Prior to GC and GC-MS analyses, these compounds were silylized. This permitted co-injection with standards and the comparison of retention values and mass spectra with those recorded for standards. The content of catechin and epicatechin in apple peel is discussed in relation to the resistance of apple trees to scab.

Keywords

catechins apple scab refractometric detector GC GC-MS 
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References

  1. Amarowicz, R., & Shahidi, F. (1996). A rapid chromatographic method for separation of individual catechins from green tea. Food Research International, 29, 71–76. DOI: 10.1016/0963-9969(95)00048-8.CrossRefGoogle Scholar
  2. Arts, I. C. W., van de Putte, B., & Hollman, P. C. H. (2000). Catechin contents of foods commonly consumed in the Netherlands. Tea, wine, fruit juices, and chocolate milk. Journal of Agricultural and Food Chemistry, 48, 1752–1757. DOI: 10.1021/jf000026+.CrossRefGoogle Scholar
  3. Boyer, J., & Liu, R. H. (2004). Apple phytochemicals and their health benefits. Nutrition Journal, 3:5. DOI: 10.1186/1475-2891-3-5.CrossRefGoogle Scholar
  4. Burda, S., Oleszek, W., & Lee, C. Y. (1990). Phenolic compounds and their changes in apples during maturation and cold storage. Journal of Agricultural and Food Chemistry, 38, 945–948. DOI: 10.1021/jf00094a006.CrossRefGoogle Scholar
  5. Dalluge, J. J., & Nelson, B. C. (2000). Determination of tea catechins. Journal of Chromatography A, 881, 411–424. DOI: 10.1016/S0021-9673(00)00062-5.CrossRefGoogle Scholar
  6. Escarpa, A., & González, M. C. (1998). High-performance liquid chromatography with diode-array detection for the determination of phenolic compounds in peel and pulp from different apple varieties. Journal of Chromatography A, 823, 331–337. DOI: 10.1016/S0021-9673(98)00294-5.CrossRefGoogle Scholar
  7. Friedman, M. (2007). Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Molecular Nutrition & Food Research, 51, 116–134. DOI: 10.1002/mnfr.200600173.CrossRefGoogle Scholar
  8. Golding, J. B., McGlasson, W. B., Wyllie, S. G., & Leach, D. N. (2001). Fate of apple peel phenolics during cool storage. Journal of Agricultural and Food Chemistry, 49, 2283–2289. DOI: 10.1021/jf0015266.CrossRefGoogle Scholar
  9. Gómez-Alonso, S., García-Romero, E., & Hermosín-Gutiérrez, I. (2007). HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence. Journal of Food Composition and Analysis, 20, 618–626. DOI: 10.1016/j.jfca.2007.03.002.CrossRefGoogle Scholar
  10. Guyot, S., Marnet, N., Sanoner, P., & Drilleau, J.-F. (2003). Variability of the polyphenolic composition of cider apple (Malus domestica) fruits and juices. Journal of Agricultural and Food Chemistry, 51, 6240–6247. DOI: 10.1021/jf0301798.CrossRefGoogle Scholar
  11. Leja, M., Mareczek, A., & Ben, J. (2003). Antioxidant properties of two apple cultivars during long-term storage. Food Chemistry, 80, 303–307. DOI: 10.1016/S0308-8146(02)00263-7.CrossRefGoogle Scholar
  12. Meszka, B., & Bielenin, A. (2006). Effect of saprotrophic microorganisms on the development of Venturia inaequalis pseudothecia. Zeszyty Naukowe Instytutu Sadownictwa i Kwiaciarstwa, 14. (in Polish).Google Scholar
  13. Mikulič Petkovšek, M. M., Štampar, F., & Veberič, R. (2009). Changes in the inner quality parameters of apple fruit from technological to edible maturity. Acta Agriculturae Slovenica, 93, 17–29. DOI: 10.2478/v10014-009-0003-3.CrossRefGoogle Scholar
  14. Montealegre, R. R., Peces, R. R., Vozmediano, J. L. C., Gascueña, J. M., & Romero, E. G. (2006). Phenolic compounds in skins and seeds of ten grape Vitis vinifera varieties grown in a warm climate. Journal of Food Composition and Analysis, 19, 687–693. DOI: 10.1016/j.jfca.2005.05.003.CrossRefGoogle Scholar
  15. Napolitano, A., Cascone, A., Graziani, G., Ferracane, R., Scalfi, L., Di Vaio, C., Ritieni, A., & Fogliano, V. (2004). Influence of variety and storage on the polyphenol composition of apple flesh. Journal of Agricultural and Food Chemistry, 52, 6526–6531. DOI: 10.1021/jf049822w.CrossRefGoogle Scholar
  16. Saito, S. T., Fröehlich, P. E., Gosmann, G., & Bergold, A. M. (2007). Full validation of a simple method for determination of catechins and caffeine in Brazilian green tea (Camellia sinensis var. assamica) using HPLC. Chromatographia, 65, 607–610. DOI: 10.1365/s10337-007-0190-1.Google Scholar
  17. van der Sluis, A. A., Dekker, M., de Jager, A., & Jongen, W. M. F. (2001). Activity and concentration of polyphenolic antioxidants in apple: Effect of cultivar, harvest year, and storage conditions. Journal of Agricultural and Food Chemistry, 49, 3606–3613. DOI: 10.1021/jf001493u.CrossRefGoogle Scholar
  18. Spanos, G. A., Wrolstad, R. E., & Heatherbell, D. A. (1990). Influence of processing and storage on the phenolic composition of apple juice. Journal of Agricultural and Food Chemistry, 38, 1572–1579. DOI: 10.1021/jf00097a031.CrossRefGoogle Scholar
  19. Vallés, B. S., Victorero, J. S., Alonso, J. J. M., & Gomis, D. B. (1994). High-performance liquid chromatography of the neutral phenolic compounds of low molecular weight in apple juice. Journal of Agricultural and Food Chemistry, 42, 2732–2736. DOI: 10.1021/jf00048a016.CrossRefGoogle Scholar
  20. Vrhovsek, U., Rigo, A., Tonon, D., & Mattivi, F. (2004). Quantitation of polyphenols in different apple varieties. Journal of Agricultural and Food Chemistry, 52, 6532–6538. DOI: 10.1021/jf049317z.CrossRefGoogle Scholar

Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2010

Authors and Affiliations

  • Marek Gołębiowski
    • 1
  • Edmund Maliński
    • 1
  • Małgorzata Szankin
    • 1
  • Mariusz Marszeniuk
    • 1
  • Monika Paszkiewicz
    • 1
  • Piotr Stepnowski
    • 1
  1. 1.Faculty of ChemistryUniversity of GdańskGdańskPoland

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