Comparative studies on the biochemical composition and polypeptide profiles of the cyst walls from sterile and fertile hydatid cysts of Echinococcus granulosus from buffalo host

Helminthologia volume 48, Article number: 88 (2011) Cite this article

Summary

In the present study, biochemical composition and polypeptide profiles of the cyst walls of sterile and fertile hydatid cyst, collected from the lungs of naturally infected Bubalus bubalis have been investigated. Significant quantitative differences in various biochemical components as well as in protein banding patterns were observed between the two types of cyst walls, suggesting variations in their metabolic states. The cyst wall collected from fertile cysts had significantly less glycogen, lipids and triglycerides but more RNA and DNA as compared to sterile cyst wall. Among phospholipid fractions, lysophosphatidylethanolamine and phosphatidylethanolamine were significantly higher in the fertile, whereas, sphingomyelin showed higher values in the sterile cyst wall. The numbers of polypeptides resolved in the fertile cyst wall were more than those in sterile cyst wall possibly due to the presence of extra proteins required for production of brood capsules and protoscoleces. Detection of more polypeptides by silver than CBBR-250 staining could be due to high sensitivity of silver stain which also detects conjugated proteins such as lipo-, phospho- and glycoproteins. The characteristic polypeptides specific to either types of the cyst wall have the potential to be exploited for diagnostics.

This is a preview of subscription content, access via your institution.

References

  1. Abidi, S. M. A., Nizami, W. A., Khan, P., Ahmad, M., Irshadullah, M. (1989): Biochemical characterization of Taenia hydatigena cysticerci from goats and pigs. J. Helminthol., 63: 333–337

    PubMed  Article  CAS  Google Scholar 

  2. Ali-Khan, Z., Siboo, R. (1981): Echinococcus multilocularis: Distribution and persistence of specific host immunoglobulins on cyst membranes. Exp. Parasitol., 51: 159–168

    PubMed  Article  CAS  Google Scholar 

  3. Barrett, J. (1981): Biochemistry of parasitic helminthes. Mcmillan Publishers Ltd., London, 307pp.

    Google Scholar 

  4. Coltorti, E. A., Varela-Diaz, V. M. (1974): Echinococcus granulosus: Penetration of macromolecules and their localization on the parasite membranes of cysts. Exp. Parasitol., 35: 225–231. DOI: 10.1016/0014-4894(74)90026-5

    Article  Google Scholar 

  5. Dzandu, J. K., Mercy, E. D., Eenise, L. B., Garry, E. W. (1984): Detection of erythrocyte membrane proteins, sialoglycoproteins and lipids in the same polyacrylamide gel using a double staining technique. Proc. Nat. Acad. Sci. U.S.A., 81: 1733–1737

    Article  CAS  Google Scholar 

  6. Frayha, G. J. (1968): A study on the synthesis and absorption of cholesterol in hydatid cysts (Echinococcus granulosus). Comp. Biochem. Physiol., 27B: 875–878

    Google Scholar 

  7. Frayha, G. J. (1974): Synthesis of certain cholesterol precursors by hydatid protoscolices of Echinococcus granulosus, E. multilocularis and cysticerci of Taenia hydatigena. Comp. Biochem. Physiol., 49B: 93–98

    Google Scholar 

  8. Gholami, S., Irshadullah, M., Mobedi, I. (2010): Rostellar hook morphology of larval Echinococcus granulosus isolates from the Indian buffalo and Iranian sheep, cattle amd camel. J. Helminthol., 1–7. DOI: 10.1017/S0022149X10000520.

  9. Ginger, C. D., Fairbairn, D. (1966): Lipid metabolism in helminth parasites. II. The major origins of the lipids of Hymenolepis diminuta (Cestoda). J. Parasitol., 52: 1097–1107

    PubMed  Article  CAS  Google Scholar 

  10. Ingold, K., Gottstein, B., Hemphill, A. (1998): Identification of a laminated layer-associated protein in Echinococcus multilocularis metacestodes. Parasitology, 116: 363–372

    PubMed  Article  CAS  Google Scholar 

  11. Irie, S., Sezaki, M., Kato, Y. (1982): A faithful double stain of proteins in the polyacrylamide gels with coomassie blue and silver. Anal. Biochem., 126: 350–354

    PubMed  Article  CAS  Google Scholar 

  12. Irshadullah, M., Nizami, W. A. (1997a): A case study of human hepatic hydatidosis and the biochemical profile of cyst wall and fluid. Ind. J. Commun. Health, 9,1: 47–52

    Google Scholar 

  13. Irshadullah, M., Nizami, W. A. (1997b): Biochemical characterization of protoscoleces isolated from buffalo hepatic and pulmonary hydatid cyst. J. Parasit. Anim. Biol. 6, 1: 13–24

    CAS  Google Scholar 

  14. Irshadullah, M., Rani, M. (2010): Histochemical studies on the metacestode of Echinococcus granulosus from Indian buffalo origin. J. Vet. Parasitol., 24,1: 33–37

    Google Scholar 

  15. Kilejian, A., Schwabe, C. W. (1971): Studies on the polysaccharides of the Echinococcus granulosus cysts, with observations on a possible mechanism of laminated membrane formation. Comp. Biochem. Physiol. 40B: 25–36

    Google Scholar 

  16. Kilejian, A., Schinazi, L. A., Schwabe, C. W. (1961): Host-parasite relationship in echinococcosis. V. Histochemical observation on Echinococcus granulosus. J. Parasitol. 47: 181–188

    PubMed  Article  CAS  Google Scholar 

  17. Laemmli, U. K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680–685. DOI: 10.1038/227680a0

    PubMed  Article  CAS  Google Scholar 

  18. Leducq, R., Gabrion, C. (1992): Developmental changes of Echinococcus multilocularis metacestodes revealed by tegumental ultrastructure and lectin-binding sites. Parasitology, 104: 129–141

    PubMed  Article  Google Scholar 

  19. Mcmanus, D. P., Bryant, C. (1995): Biochemistry, physiology and molecular biology of Echinococcus. In: Thompson, R. C. A., Lymbery, A. J. (Eds.) Echinococcus and hydatid disease. CAB International, Wallingford, UK, pp. 135–181

    Google Scholar 

  20. Richards, K. S., Ilderton, E., Yardley, H. J. (1987): Lipids in the laminated layer of liver, lung and daughter hydatid cysts of equine Echinococcus granulosus (Cestoda). Comp. Biochem. Physiol. 86B: 209–212. DOI: 10.1016/0305-0491(87)90199-4.

    CAS  Google Scholar 

  21. Rouser, G., Fleischer, S., Yamamoto, A. (1970): Two dimensional thin layer chromatographic separations of polar lipids and determination of phospholipids by phosphorous analysis of spots. Lipids, 5: 494–496

    PubMed  Article  CAS  Google Scholar 

  22. Sadanand, A. V. (1971): Biochemical analyses of the cyst wall of Echinococcus granulosus Batsch. Comp. Biochem. Physiol. 40B: 797–805. DOI: 10.1016/0305-0491(71)90153-2.

    Google Scholar 

  23. Skipski, V. P., Peterson, R. F, Barclay, M. (1964): Quantitative analysis of phospholipids by thin layer chromatography. Biochem. J., 90: 374–378

    PubMed  CAS  Google Scholar 

  24. Smith, F. F., Walker, C. W. (1986): Biochemical changes in the composition of the testes during spermatogenesis in the sea star Asterias vulgaris. J. Exp. Zool., 237: 351–364. DOI: 10.1002/jez.1402370308.

    Article  CAS  Google Scholar 

  25. Smyth, J. D., Mcmanus, D. P. (1989): The Physiology and Biochemistry of Cestodes. Cambridge University Press, Cambridge, 367pp.

    Google Scholar 

  26. Sørensen, B. K., Højrup, P., Østergård, E., Jørgensen, C. S., Enghild, J., Ryder, L. R., Houen, G. (2002): Silver staining of proteins on electroblotting membranes and intensification of silver staining of proteins separated by polyacrylamide gel electrophoresis. Anal. Biochem., 304: 33–41. DOI: 10.1006/abio.2001.5604

    PubMed  Article  Google Scholar 

  27. Spector, T. (1978): Refinement of the coomassie blue method of protein quantitation. Anal. Biochem., 86: 142–146. DOI: 10.1016/0003-2697(78)90327-5

    PubMed  Article  CAS  Google Scholar 

  28. Taherkhani, H., Rogan, M. T. (2000): General characterization of laminated layer of Echinococcus granulosus. Iran. J. Med. Sci., 25: 95–104

    Google Scholar 

  29. Thompson, R. C. A. (1995): Biology and systematics of Echinococcus. In: Thompson, R. C. A., Lymbery, A. J. (Eds.) Echinococcus and hydatid disease. CAB International, Wallingford, UK, pp. 1–50

    Google Scholar 

  30. Thompson, R. C. A., Mcmanus, D. P. (2002): Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 18: 452–457

    PubMed  Article  Google Scholar 

  31. Turcekova, L., Snabel, V., Dudinak, V., Gaspar, V., Dubinsky, P. (2009): Prevalence of cystic echinococcosis in pigs from Slovakia, with evaluation of size, fertility and number of hydatid cysts. Helminthologia, 46,3: 151–158. DOI: 10.2478/s11687-009-0029-4

    Article  Google Scholar 

  32. Varela-Diaz, V. M., Coltorti, E. A. (1973): The presence of host immunoglobulins in hydatid cyst membranes. J. Parasitol., 59: 484–488

    PubMed  Article  CAS  Google Scholar 

  33. Vercelli-Retta, J., Reissenweber, N. J., Loranzo, W., Siri, A. M. (1975): Histochemistry and histoenzymology of the hydatid cyst. (Echinococcus granulosus Batsch 1786). Parasitol. Res., 48: 15–23. DOI: 10.1007/BF00389825

    CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India

    M. Irshadullah & M. Rani

Authors
  1. M. Irshadullah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Rani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Irshadullah.

About this article

Cite this article

Irshadullah, M., Rani, M. Comparative studies on the biochemical composition and polypeptide profiles of the cyst walls from sterile and fertile hydatid cysts of Echinococcus granulosus from buffalo host. Helminthologia 48, 88 (2011). https://doi.org/10.2478/s11687-011-0015-5

Download citation

Keywords

  • Biochemical composition
  • cyst wall
  • SDS-gradient PAGE
  • polypeptides