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Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran–Iraq

  • Arjang Partoandazanpoor
  • Zainab Sadeghi-DehkordiEmail author
  • Loghman Ekradi
  • Monireh Khordadmehr
  • Maryam Rassouli
  • Alireza Sazmand
Original Paper
  • 9 Downloads

Abstract

Background

Infection with the protozoan parasite Toxoplasma gondii is widely prevalent in animals and humans. In goats and sheep this infection has a high economic impact as it causes abortions and lamb losses. Although there are several studies reporting seroprevalence in small ruminants in Iran, molecular-based information is scarce.

Purpose

This study aimed to screen caprine and ovine aborted fetuses for T. gondii infection by PCR and histopathology.

Methods

Brains of 121 aborted fetuses (10 caprine and 111 ovine) were collected from different parts of the Kordestan province, bordering with Iraq. Gestational age and the general status of each fetus such as freshness, autolysis, mummification and presence of macroscopic lesions in the fetus and foetal membranes was recorded. Individual brain tissues of fetuses were subjected to nested-PCR targeting the B1 gene, and histopathological sections prepared from brains were examined microscopically.

Results

PCR results revealed T. gondii-associated abortion in one caprine and nine ovine fetuses (8.3%). Microscopically, pathological lesions included non-purulent meningitis associated with gliosis, focal necrosis, and occasionally tissue cyst.

Conclusion

This paper reports for the first time T. gondii-associated abortion in goats in Iran. As organs from aborted fetuses do not necessarily show lesions, molecular confirmation is the unique diagnostic method and should be used in situations of an abortion epidemic.

Keywords

Toxoplasmosis Zoonosis Parasitic infection Small ruminants PCR Histopathology 

Notes

Acknowledgements

This study was partially supported by Bu-Ali Sina University of Hamedan, Iran. The authors wish to thank Sakineh Azami for her kind assistance in the laboratory, and Dr. Ali Sadeghinasab for his help in statistical analyses.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of Pathobiology, Faculty of Veterinary ScienceBu-Ali Sina UniversityHamedanIran
  2. 2.Department of Pathobiology, Faculty of Veterinary MedicineIslamic Azad University, Sanandaj BranchSanandajIran
  3. 3.Department of Pathobiology, Faculty of Veterinary MedicineUniversity of TabrizTabrizIran
  4. 4.Department of Pathobiology, Faculty of Veterinary MedicineSemnan UniversitySemnanIran

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