Acta Parasitologica

, Volume 59, Issue 4, pp 767–772 | Cite as

Molecular identification larvae of Onchobothrium antarcticum (Cestoda: Tetraphyllidea) from marbled rockcod, Notothenia rossii, in Admiralty Bay (King George Island, Antarctica)

  • Zdzislaw LaskowskiEmail author
  • Anna Rocka
Research Note


Antarctic bony fishes are infected with cestode larvae belonging to the order Tetraphyllidea (parasites as adults in chondrichthyans). Larvae of the Tetraphyllidea differ from each other in the morphology of their scoleces and represent five forms. There are larvae with bothridia subdivided into one, two and three loculi, bothridia sac-like in shape and bothridia undivided with hook-like projections. Only one species of the family Onchobothriidae, Onchobothrium antarcticum, has been described from Antarctica and larvae with trilocular bothridia were assigned to this cestode species. In this study, ten larvae obtained from Notothenia rossii and three adult specimens of Onchobothrium antarcticum isolated from Bathyraja eatonii were examined. A partial sequence of cytochrome c oxidase subunit 1 of three adult specimens and four larvae was identical. The remaining six larval sequences differed from the sequences obtained from adult cestodes. Partial sequences of lsrDNA of all analyzed larvae were identical. These results confirm the taxonomic affiliation of the larvae with trilocular bothridia parasitizing marbled rockcod in Antarctica as Onchobothrium antarcticum.


molecular identification Onchobothrium antarcticum Antarctica 


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  1. Agusti C., Aznar F., Olson P.D., Littlewood D.T.J., Kostadinova A., Raga J.A. 2005. Morphological and molecular characterization of tetraphyllidean merocercoids (Platyhelminthes: Cestoda) of striped dolphins (Stenella coeruleoalba) from the Western Mediterranean. Parasitology, 130, 1–14. DOI:10.1017/S0031182004006754.CrossRefGoogle Scholar
  2. Avdeeva N.V. 1989. On the generic belonging of three types of larvae of cestodes of the collective genus „Scolex”. Parazitologiya, 23, 351–355 (In Russian).Google Scholar
  3. Avdeeva N.V., Avdeev V.V. 1980. Peculiarities of morphogenesis of adhesive organs of some plerocercoids of the collective genus Scolex (Tetraphyllidea) and their identification. Parazitologiya, 14, 242–250 (In Russian).Google Scholar
  4. Aznar F.J., Agusti C., Littlewood D.T.J., Raga J.A., Olson P.D. 2007. Insight into the role of cetaceans in the life cycle of the tetraphyllideans (Platyhelminthes: Cestoda). International Journal for Parasitology, 37, 243–255.CrossRefPubMedGoogle Scholar
  5. Brickle P., Olson P.D., Littlewood D.T.J., Bishop A., Arkhipkin A.I. 2001. Parasites of Loligo gahi from waters off the Falkland Islands, with a phylogenetically based identification of their larvae. Canadian Journal of Zoology, 79, 2289–2296. DOI: 10.1139/cjz-79-12-2289.CrossRefGoogle Scholar
  6. Caira J.N., Jensen K., Waeschenbach A., Olson P.D., Littlewood T.J. 2014. Orders out of chaos — molecular phylogenetics reveals the complexity of shark and stingray tapeworm relationships. International Journal for Parasitology, 44, 55–73. DOI: 10.1016/j.ijpara.2013.10.004.CrossRefPubMedGoogle Scholar
  7. Carvajal J., Barros C., Santander G. 1982. In vitro culture of Rhodobothrium mesodesmatum (Cestoda: Tetraphyllidea), parasite of a Chilean clam. Proceedings of the Helminthological Society of Washington, 49, 226–230.Google Scholar
  8. Chambers C.B., Cribb T.H., Jones M.K. 2000. Tetraphyllidean metacestodes of teleosts of the Great Barrier Reef, and the use of in vitro cultivation to identify them. Folia Parasitologica, 47, 285–292.CrossRefPubMedGoogle Scholar
  9. Darriba D., Taboada G.L., Doallo R., Posada D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772. DOI:10.1038/nmeth.2109.CrossRefPubMedPubMedCentralGoogle Scholar
  10. Gon O., Heemstra P.C. eds. 1990. Fishes of the Southern Ocean. J.L.B. Smith Institute of Ichthyology, Grahamstown, 462 pp.Google Scholar
  11. Guindon S., Gascuel O. 2003. A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology, 52, 6960704. DOI: 10.1080/10635150390235520.CrossRefGoogle Scholar
  12. Hamilton K.A., Byram J.E. 1974. Tapeworm development: The effects of urea on a larval tetraphyllidean. Journal of Parasitology, 60, 20–28.CrossRefPubMedGoogle Scholar
  13. Holland N.D., Wilson N.G. 2009. Molecular identification of larvae of a tetraphyllidean tapeworm (Platyhelminthes: Eucestoda) in a razor clam as an alternative intermediate host in the life cycle of Acanthobothrium brevissime. Journal of Parasitology, 95, 1215–1217. DOI:10.1645/GE-1946.1.CrossRefPubMedGoogle Scholar
  14. Holland N.D., Campbell T.G., Garey J.R., Holland L.Z., Wilson N.G. 2009. The Florida amphioxus (Cephalochordata) hosts larvae of the tapeworm Acanthobothrium brevissime: natural history, anatomy and taxonomic identification of the parasite. Acta Zoologica, 90, 75–86. DOI:.x.CrossRefGoogle Scholar
  15. Huelsenbeck J.P., Ronquist F. 2001. MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755. DOI: 10.1093/bioinformatics/17.8.754.CrossRefPubMedGoogle Scholar
  16. Jensen K., Bullard S.A. 2010. Characterization of a diversity of tetraphyllidean and rhinebothriidean cestode larval types, with comments on host associations and life-cycles. International Journal for Parasitology, 40, 889–910. DOI: 10.1016/j.ijpara.2009.11.015.CrossRefPubMedGoogle Scholar
  17. Leiper R.T., Atkinson E.L. 1914. Helminthes of the British Antarctic Expedition, 1910–1913. Proceedings of the Zoological Society of London, 1, 222–226.Google Scholar
  18. Leiper R.T., Atkinson E.L. 1915. Parasitic worms, with a note on a free-living nematode. British Antarctic „Terra Nova” Expedition. Natural History Report, Zoology, 2, 19–60.Google Scholar
  19. Prudhoe S. 1969. Cestodes from fish, birds and whales. Reports B.A.N.Z. Antarctic Research Expedition 1929–1931, 8B, 171–193.Google Scholar
  20. Reimer L.V. 1975. Cestodenlarven in Wirbellosen der Küste von Madras. Angewandte Parasitologie, 1, 2–6.Google Scholar
  21. Reimer L.V. 1977. Larval cestodes in plankton invertebrates of the Atlantic near the shore of north-west Africa. Parazitologiya, 11, 309–315 (In Russian).Google Scholar
  22. Reimer L.V. 1987. Helminthen von Fischen der Antarktis. Fischerei-Forschung, Rostock, 25, 36–40.Google Scholar
  23. Rocka A. 2003. Cestodes of the Antarctic fishes. Polish Polar Research, 24, 261–276.Google Scholar
  24. Southwell T. 1925. A monograph on the Tetraphyllidea with notes on related cestodes. Memoir Liverpool School Tropical Medicine, 2, 368 pp.Google Scholar
  25. Waeschenbach, A., Webster, B.L., Bray, R.A., Littlewood, D.T.J. 2007. Added resolution among ordinal level relationships of tapeworms (Platyhelminthes: Cestoda) with complete small and large subunit nuclear ribosomal RNA genes. Molecular Phylogenetics and Evolution, 45, 311–325. DOI:10.1016/j.ympev.2007.03.019.CrossRefPubMedGoogle Scholar
  26. Wojciechowska A. 1990. Onchobothrium antarcticum sp. n. (Tetraphyllidea) from Bathyraja eatonii (Günther, 1876) and a plerocercoid from Notothenioidea (South Shetlands, Antarctic). Acta Parasitologica, 35, 113–117.Google Scholar
  27. Wojciechowska A. 1993. The tetraphyllidean and tetrabothriid cercoids from Antarctic bony fishes. I. Morphology. Identification with adult forms. Acta Parasitologica, 38, 15–22.Google Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  1. 1.W. Stefański Institute of ParasitologyPolish Academy of SciencesWarsawPoland

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