Acta Parasitologica

, Volume 57, Issue 3, pp 273–284 | Cite as

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

  • Kateřina Huňová
  • Martin Kašný
  • Vladimír Hampl
  • Roman Leontovyč
  • Aleš Kuběna
  • Libor Mikeš
  • Petr Horák
Original Paper

Abstract

Lymnaeid snails of the genus Radix serve as intermediate hosts of some schistosomes and fasciolids. In Europe, delineation of species within the genus Radix is unresolved and, therefore, spectrum of snail hosts susceptible to trematode infections is under discussion. We used and compared three criteria for species delineation using snails collected at 43 localities. (a) Sequence analysis of ITS-2 rDNA disclosed that the collected snails belong to four species — R. auricularia (Linnaeus, 1758), R. peregra (Müller, 1774), R. lagotis (Schrank, 1803) and R. labiata (Rossmaessler, 1835) (criteria and names are based on the work of Bargues et al. 2001). Occurrence of R. peregra in the Czech Republic was confirmed by molecular data for the first time. (b) Characterization of reproductive system disclosed differences in location, size and shape of bursa copulatrix and its ductus. Unfortunately, some R. labiata specimens shared morphological features of reproductive organs with R. lagotis. (c) Statistical analysis of shell morphology proved that significant differences exist among particular species. One prediction model showed that correct classification of species may be achieved in 82-84% of cases. However, identification of individual snails in the field (without knowledge of respective snail population and use of statistical tools) still remains a complicated issue due to overlaps of shell characteristics. Concerning the role in trematode transmission, R. lagotis, R. labiata and R. peregra are susceptible to Trichobilharzia regenti. Also, successful experimental infections of R. lagotis and R. labiata by Fascioloides magna were accomplished.

Keywords

Lymnaeidae Radix Trichobilharzia Fascioloides trematodes species delineation 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Kateřina Huňová
    • 1
    • 3
  • Martin Kašný
    • 1
  • Vladimír Hampl
    • 1
  • Roman Leontovyč
    • 1
  • Aleš Kuběna
    • 2
  • Libor Mikeš
    • 1
  • Petr Horák
    • 1
  1. 1.Faculty of Science, Department of ParasitologyCharles University in PraguePrague 2Czechoslovakia
  2. 2.Faculty of Science, Department of Philosophy and History in SciencesCharles University in PraguePrague 2Czechoslovakia
  3. 3.Faculty of Science, Department of Education and Didactics of BiologyCharles University in PraguePrague 2Czechoslovakia

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