Acta Parasitologica

, Volume 57, Issue 3, pp 316–322 | Cite as

Morphological and molecular characterization of Raphidascaris (Ichthyascaris) lophii (Wu, 1949) (Nematoda, Anisakidae) from marine fishes from China, with a key to the species of the subgenus Ichthyascaris

  • Zhen Xu
  • Lu-Ping Zhang
  • Bao-Chen Liu
  • Liang Li
Original Paper


The little known ascaridoid nematode Raphidascaris (Ichthyascaris) lophii (Wu, 1949) is redescribed and illustrated based on newly collected specimens from the five different marine fishes: Lophius litulon (Jordan) (Lophiiformes: Lophiidae), Lophiomus setigerus (Vahl) (Lophiiformes: Lophiidae), Antennarius hispidus (Bloch et Schneider) (Lophiiformes: Antennaridae), Zeus faber Linnaeus (Zeiformes: Zeidae) and Ostichthys japonicus (Cuvier) (Beryciformes: Holocentridae) from the East and South China Sea. This species differs from all congeners in the subgenus Ichthyascaris by the length of the ventricular appendix (0.52–0.98 mm long), the number and arrangement of caudal papillae (26–32 pairs of precloacal, 3–4 pairs of paracloacal and 8–11 pairs of postcloacal) and the length of the spicules (0.49–0.88 mm long, representing 3.08–4.70% of body length). In addition, nematodes collected from these five different fishes have been characterized using molecular methods by sequencing and analysing the internal transcribed spacer (ITS) of ribosomal DNA. No variation in size and nucleotide polymorphisms is detected within the target sequence among all samples analysed. These data contribute to facilitate an accurate diagnosis of this poorly known nematode. An identification key to the species of the subgenus Ichthyascaris is also provided.


Ascaridida marine fishes Raphidascaris Ichthyascaris internal transcribed spacer (ITS) 


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  1. Bruce N.L. 1990. Hysterothylacium Ward and Magath, 1917, and Ichthyascaris Wu, 1949, ascaridoid nematodes from Australian demersal fishes. Memoirs of the Queensland Museum, 28, 389–426.Google Scholar
  2. Bruce N.L., Adlard R.D., Cannon L.R.G. 1994. Synoptic checklist of ascaridoid parasites (Nematoda) from fish hosts. Invertebrate Taxonomy, 8, 583–674. DOI: 10.1071/IT9940583.CrossRefGoogle Scholar
  3. Chabaud A.G. 1965. Ordre des Ascaridida. In: (Ed. Grassé P.-P), Traité de Zoologie. Tome IV, Fascicule 3, Masson et Cie, Paris, pp. 932–1025.Google Scholar
  4. Du C.X., Zhang L.P., Shi M.Q., Ming Z., Hu M., Gasser R.B. 2010. Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach. Electrophoresis, 31, 654–658. DOI:10.1002/elps.200900531.PubMedCrossRefGoogle Scholar
  5. Fang W.Z., Xu S.S., Zhang S.L., Wang Y.N., Chen X.B., Luo D.M. 2010. Multiple primer PCR for the identification of anisakid nematodes from Taiwan Strait. Experimental Parasitology, 124, 197–201. DOI: 10.1016/j.exppara.2009.09.011.PubMedCrossRefGoogle Scholar
  6. Hartwich G. 1957. Zur Systematik der Nematoden — Superfamilie Ascaridoidea. Zoologische Jahrbücher, Jena (Systematik, Ökologie und Geographie), 85, 211–252.Google Scholar
  7. Hooper J.N.A. 1983. Parasities of estuarine and oceanic flathead fishes (Family Platycephalidae) from northern New South Wales. Australian Journal of Zoology Supplementary Series, 90, 1–69. DOI: 10.1071/AJZS090.CrossRefGoogle Scholar
  8. Lèbre C., Petter A.J. 1983. Deux nouvelle espèces d’Ascarides (Nematoda) parasites de poisons Téléostéens: Raphidascaris mediterraneus n. sp. et Goezia anguillae n. sp.; complément de description de Cucullanus micropapillatus Tornquist, 1931 (Nematoda, Cucullanidae). Bulletin du Muséum National d’Histoire Naturelle, Paris, 4e Sér., 5, Sect. A, 2, 491–505.Google Scholar
  9. Li L., Gibson D.I., Liu Y.Y., Zhang L.P. 2012. Morphological and molecular study of the poorly known species Pseudanisakis rajae (Yamaguti, 1941) (Nematoda: Acanthocheilidae) from elasmobranchs in the Yellow Sea and Taiwan Strait off the coast of China. Systematic Parasitology, 81, 115–123. DOI: 10.1007/s11230-011-9331-x.PubMedCrossRefGoogle Scholar
  10. Luo D., Huang H. 2001. Heliconema minnanensis n. sp. (Physalopteroidea: Physalopteridae) and Raphidascaris trichiuri (Yin and Zhang) n. comb. (Ascaridoidea: Anisakidae) in marine fishes. Journal of Parasitology, 87, 1090–1094.Google Scholar
  11. Moravec F. 1994. Parasitic nematodes of freshwater fishes of Europe. Prague et Dordrecht, Boston, London: Academia et Kluwer Academic Publishers 473 pp.Google Scholar
  12. Moravec F., Nagasawa K. 2002. Redescription of Raphidascaris gigi Fujita, 1928 (Nematoda: Anisakidae), a parasite of freshwater fishes in Japan. Systematic Parasitology, 52, 193–198.PubMedCrossRefGoogle Scholar
  13. Moravec F., Justine J.L. 2005. Two anisakid nematodes from marine fishes off New Caledonia, including Raphidascaris (Ichthyascaris) nemipteri n. sp. from Nemipterus furcosus. Systematic Parasitology, 62, 101–110. DOI: 10.1007/s11230-005-5484-9.PubMedCrossRefGoogle Scholar
  14. Olsen L.S. 1952. Some nematodes parasitic in marine fishes. Publications of the Institute of Marine Science, University of Texas, 2, 173–215.Google Scholar
  15. Santos E. 1970. Nova espécie do gênero Raphidascaris, Railliet and Henry, 1915 (Nematoda, Ascaridoidea). Atas da Sociedade de Biologia de Rio de Janeiro, 14, 39–40.Google Scholar
  16. Smith J.D. 1984. Taxonomy of Raphidascaris spp. (Nematoda, Anisakidae) of fishes, with a redescription of R. acus (Bloch, 1772). Canadian Journal of Zoology, 62, 685–694. DOI: 10.1139/z84-100.CrossRefGoogle Scholar
  17. Testini G., Papini R., Lia R.P., Parisi A., Dantas-Torres F., Traversa D., Otranto D. 2011. New insights into the morphology, molecular characterization and identification of Baylisascaris transfuga (Ascaridida, Ascarididae). Veterinary Parasitology, 175, 97–102. DOI: 10.1016/j.vetpar.2010.09.017.PubMedCrossRefGoogle Scholar
  18. Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680. DOI: 10.1093/nar/22.22.4673.PubMedCrossRefGoogle Scholar
  19. Yamaguti S. 1935. Studies on the helminth fauna of Japan. Part 9. Nematodes of fishes. 1. Japanese journal of Zoology, 6, 337–386.Google Scholar
  20. Yin W.Z., Zhang N.X. 1983. On two new species of parasitic nematodes from marine fishes from Zhoushan Islands, China. Acta Zootaxonomic Sinica, 8, 7–10. DOI: 1000-0739.0.1983-01-002.Google Scholar
  21. Wu H.W. 1949. A note on two parasitic nematodes of fishes. Sinensia, 20. 51–57.Google Scholar
  22. Zhang L.P., Hu M., Shamsi S., Beveridge I., Li H.M., Xu Z., Li L., Cantacessi C., Gasser R.B. 2007. The specific identification of anisakid larvae from fishes from the Yellow Sea, China, using mutation scanning-coupled sequence analysis of nuclear ribosomal DNA. Molecular and Cellular Probes, 21, 386–390. DOI: 10.1016/j.mcp.2007.05.004.PubMedCrossRefGoogle Scholar
  23. Zhu X.Q., Podolska M., Liu J.S., Yu H.Q., Chen H.H., Lin Z.X., Luo C.B., Song H.Q., Lin R.Q. 2007. Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA. Parasitology Research, 101, 1703–1707. DOI: 10.1007/s00436-007-0699-0.PubMedCrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Zhen Xu
    • 1
    • 2
  • Lu-Ping Zhang
    • 2
  • Bao-Chen Liu
    • 3
  • Liang Li
    • 2
  1. 1.College of Medical ScienceHebei Engineering UniversityHandan, Hebei ProvinceP. R. China
  2. 2.College of Life ScienceHebei Normal UniversityShijiazhuang, Hebei ProvinceP. R. China
  3. 3.Botou Vocational CollegeBotou, Hebei ProvinceP. R. China

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