Acta Parasitologica

, Volume 57, Issue 3, pp 293–296 | Cite as

Molecular identification of Diphyllobothrium latum and a brief review of diphyllobothriosis in China

  • Ai-Jiang Guo
  • Kan Liu
  • Wei Gong
  • Xue-Nong Luo
  • Hong-Bin Yan
  • Song-Buo Zhao
  • Song-Nian Hu
  • Wan-Zhong Jia
Original Paper

Abstract

Two tapeworm specimens collected in northeast China in 2009 and 2011 were identified as Diphyllobothrium latum based on morphological criteria. Molecular methods were used to confirm their identity and analyze genetic variations compared with published data for this species. Species identity was confirmed by molecular characterization of the 18S rDNA partial sequence, complete sequences of internal transcribed spacers (ITSs) and 5.8S rDNA, and partial sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and mitochondrial NADH dehydrogenase subunit 5 (nad5). PCR amplification and sequence analysis of 18S rDNA (1472 bp), ITS regions (1218 bp), cox1 (885 bp), and nad5 (1028 bp) revealed that these four sequences showed more than 99% identity to reference sequences for D. latum, confirming that this species is D. latum. To date, a total of 12 diphyllobothriosis cases have been documented in China. This study represents the first molecular characterization of D. latum in China, providing molecular evidence of human diphyllobothriosis in China.

Keywords

Diphyllobothrium latum 18S rDNA ITS coxnadChina 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baer J.G., Miranda C.H., Fernancdees R.W., Median T.J. 1967. Human diphyllobothriasis in Peru. Zeitschrift für Parasitenkunde, 28, 277–289.PubMedGoogle Scholar
  2. Brabec J., Kuchta R., Scholz T. 2006. Paraphyly of the Pseudophyllidea (Platyhelminthes: Cestoda): circumscription of monophyletic clades based on phylogenetic analysis of ribosomal RNA. International Journal for Parasitology, 36, 1535–1541. DOI: 10.1016/j.ijpara.2006.08.003.PubMedCrossRefGoogle Scholar
  3. Chen B.J., Li L.S., Ling J.X., Lin Y.S., Li Y.R. 2005. A case of human diphyllobothriasis after eating Plecoglossus altivelis. Journal of Tropical Diseases and Parasitology, 3, 126.Google Scholar
  4. Chen X.T. (Ed.). 1965. Medical Parasitology. People’s Health Publishing, Beijing, China.Google Scholar
  5. Chou H.F., Yen C.M., Liang W.C., Jong Y.J. 2006. Diphyllobothriasis latum: the first child case report in Taiwan. Kaohsiung Journal of Medical Sciences, 22, 346–351. DOI: 10.1016/S1607-551X(09)70321-3.PubMedCrossRefGoogle Scholar
  6. Dick T. 2008. Diphyllobothriasis: the Diphyllobothrium latum human infection conundrum and reconciliation with a worldwide zoonosis. In: (Eds. K.D. Murrell and B. Fried) Food-borne Parasitic Zoonoses: fish and plant-borne parasites (world class parasites). Springer Publishing, London, UK, 151–184.Google Scholar
  7. Dick T.A., Nelson P.A., Choudhury A. 2001. Diphyllobothriasis: update on human cases, foci, patterns and sources of human infections and future considerations. Southeast Asian Journal of Tropical Medicine Public Health, 32, 59–76.Google Scholar
  8. Dupouy-Camet J., Peduzzi R. 2004. Current situation of human diphyllobothriasis in Europe. Euro Surveillance, 9, 31–35.PubMedGoogle Scholar
  9. Fan S.Q., Wang G.Y., Sun M.F. 1995. A case of Diphyllobothrium latum infection in Heilongjiang. Chinese Journal of Parasitology and Parasite Diseases, 13, 240.Google Scholar
  10. Faust E.C., Russell P.F. (Ed.). 1964. Craig and Faust’s Clinical Parasitology. Lea & Febiger, Philadelphia, USA, 977 pp.Google Scholar
  11. Lee E.B., Song J.H., Park N.S., Kang B.K., Lee H.S., Han Y.J., Kim H., Shin E., Chai J. 2007. A case of Diphyllobothrium latum infection with a brief review of diphyllobothriasis in the Republic of Korea. Korean Journal of Parasitology, 45, 219–223. DOI: 10.3347/kjp.2007.45.3.219.PubMedCrossRefGoogle Scholar
  12. Lin C.S., Wu L.T. 1927. Report of a preliminary health survey of pinchiang (Chinese City of Harbin). National Medicine Journal of China, 13, 24–28.Google Scholar
  13. Lou H.Y., Tsai P.C., Chang C.C., Lin Y.H., Liao C.W., Kao T.C., Lin H.C., Lee W.C., Fan C.K. 2007. A case of human diphyllobothriasis in northern Taiwan after eating raw fish fillets. Journal of Microbiology, Immunology Infection, 40, 452–456.Google Scholar
  14. Nakao M., Abmed D., Yamasaki H., Ito A. 2007. Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidae). Parasitology Research, 101, 233–236. DOI:10.1007/s00436-006-0433-3.PubMedCrossRefGoogle Scholar
  15. Osorio G., Daiber A., Donckaster R., Ubilla M., Con I., Anguita T., Pinto R. 1974. Severe megaloblastic anemia due to Diphyllobothrium latum. First case identified in Chile. Revista Medica de Chile 102, 700–703.PubMedGoogle Scholar
  16. Park J.K., Kim K.H., Kang S., Jeon H.K., Kim J.H., Littlewood D.T.J., Eom K.S. 2007. Characterization of the mitochondrial genome of Diphyllobothrium latum (Cestoda: Pseudophyllidea) — implications for the phylogeny of eucestodes. Parasitology, 134, 749–759. DOI:10.1017/S003118200600206X.PubMedCrossRefGoogle Scholar
  17. Rohela M., Jamaiah I., Chan KW., Yusoff W.S. 2002. Diphyllobothriasis: the first case report from Malaysia. Southeast Asian Journal of Tropical Medicine Public Health, 33, 229–230.Google Scholar
  18. Rohela M., Jamaiah I., Goh K.L, Nissapatorn V. 2006. A second case of diphyllobothriasis in Malaysia. Southeast Asian Journal of Tropical Medicine Public Health, 37, 896–898.Google Scholar
  19. Santos F.L.N., De Faro L.B. 2005. The first confirmed case of Diphyllobothrium latum in Brazil. Memórias do Instituto Oswaldo Cruz, 100, 685–686. DOI: 10.1590/S0074-02762005000600013.CrossRefGoogle Scholar
  20. Scholz T., Garcia H.H., Kuchta R., Wicht B. 2009. Update on the Human Broad Tapeworm (Genus Diphyllobothrium), Including Clinical Relevance. Clinical Microbiology Reviews, 22, 146–160. DOI: 10.1128/CMR.00033-08.PubMedCrossRefGoogle Scholar
  21. Semenas L., Kreiter A., Urbanski J. 2001. New cases of human diphyllobothriasis in Patagonia, Argentina. Revista de Saúde Pública, 35, 214–216. DOI: 10.1590/S0034-89102001000200017.PubMedCrossRefGoogle Scholar
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739. DOI: 10.1093/molbev/msr121.PubMedCrossRefGoogle Scholar
  23. Waloch M. 2005. Cestode infections in Poland in 2003. Przegląd Epidemiologiczny, 59, 331–335.PubMedGoogle Scholar
  24. Wicht B., Yanagida T., Scholz T., Ito A., Jiménez J.A., Brabec J. 2010. Multiplex PCR for the differential diagnosis of human broad tapeworms (Cestoda: Diphyllobothrium) infecting humans. Journal of Clinical Microbiology, 48, 3111–3116. DOI: 10.1128/JCM.00445-10.PubMedCrossRefGoogle Scholar
  25. Yera H., Nicoulaud J., Dupouy-Camet J. 2008. Use of nuclear and mitochondrial DNA PCR and sequencing for molecular identification of Diphyllobothrium isolates potentially infective for humans. Parasite, 15, 402–407.PubMedGoogle Scholar
  26. Zhang Y.R., Wu Z.Q., Liu X. 1996. A case of Diphyllobothrium latum infection. Chinese Journal of Parasitic Disease Control, 9, 211.Google Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Ai-Jiang Guo
    • 1
  • Kan Liu
    • 2
    • 3
  • Wei Gong
    • 1
  • Xue-Nong Luo
    • 1
  • Hong-Bin Yan
    • 1
  • Song-Buo Zhao
    • 1
  • Song-Nian Hu
    • 2
  • Wan-Zhong Jia
    • 1
  1. 1.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Zoonoses of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research Institute, CAASLanzhou, GansuChina
  2. 2.CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of GenomicsChinese Academy of SciencesBeijingChina
  3. 3.Graduate UniversityChinese Academy of SciencesBeijingChina

Personalised recommendations