Acta Parasitologica

, Volume 57, Issue 3, pp 211–215 | Cite as

Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

  • Benito David Mata-Cárdenas
  • Magda Elizabeth Hernández-García
  • Francisco González-Salazar
  • Jesús Norberto Garza-González
  • Rebeca Palacios-Corona
  • Elva I. Cortés-Gutiérrez
  • Javier Vargas-Villarreal
Original Paper
  • 119 Downloads

Abstract

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle’s amino acid solution, Diamond’s vitamin-tween 80 mixtures and LCR (a lipid-cholesterol — rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.

Keywords

Giardia duodenalis PACSR culture media mammalian serum phospholipase A2 activity 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Benito David Mata-Cárdenas
    • 2
  • Magda Elizabeth Hernández-García
    • 1
    • 3
  • Francisco González-Salazar
    • 1
    • 4
  • Jesús Norberto Garza-González
    • 1
  • Rebeca Palacios-Corona
    • 1
  • Elva I. Cortés-Gutiérrez
    • 1
  • Javier Vargas-Villarreal
    • 1
  1. 1.División de Biología Celular y Molecular. Centro de Investigación Biomédica del NoresteInstituto Mexicano del Seguro SocialMonterrey, Nuevo LeónMéxico
  2. 2.Facultad de Ciencias QuímicasUniversidad Autónoma de Nuevo LeónSan Nicolás de los Garza, Nuevo LeónMéxico
  3. 3.Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo LeónSan Nicolás de los Garza; Nuevo LeónMéxico
  4. 4.División de Ciencias BásicasUniversidad de MonterreySan Pedro Garza García, N. L.México

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