Acta Parasitologica

, Volume 57, Issue 3, pp 297–301 | Cite as

SEM evidence for existence of an apical disc on the scolex of Clestobothrium crassiceps (Rudolphi, 1819): comparative results of various fixation techniques

  • Jordi Miquel
  • Zdzisław Świderski
  • Adji Mama Marigo
  • Eulàlia Delgado
  • Jordi Torres
Original Paper

Abstract

The scolex of the bothriocephalidean cestode Clestobothrium crassiceps was studied by means of scanning electron microscopy (SEM). The comparative results of various fixation procedures and techniques are presented. The scolex of C. crassiceps is oval to globular and exhibits two deep bothria which appear in the form of two lobes separated by a longitudinal groove. At the apex of the scolex, resembling a beret, an apical disc is present (oval, flattened and with a sinuous edge). Our results are compared with those previously reported in other species of Clestobothrium. This study represents the first report which highlights the presence of an apical disc in the scolex of C. crassiceps. It describes the effects of different procedures applied to our material during preparation and a comparative analysis results obtained using these various methods.

Keywords

Clestobothrium crassiceps Bothriocephalidea Cestoda apical disc scolex SEM 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Jordi Miquel
    • 1
    • 2
  • Zdzisław Świderski
    • 3
    • 4
  • Adji Mama Marigo
    • 1
    • 2
  • Eulàlia Delgado
    • 5
  • Jordi Torres
    • 1
    • 2
  1. 1.Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Institut de Recerca de la Biodiversitat, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain
  3. 3.W. Stefański Institute of ParasitologyPolish Academy of SciencesWarsawPoland
  4. 4.Department of General Biology and ParasitologyWarsaw Medical UniversityWarsawPoland
  5. 5.Departament de Ciències Ambientals, Facultat de CiènciesUniversitat de GironaGironaSpain

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