Acta Parasitologica

, Volume 57, Issue 1, pp 67–73 | Cite as

Detection and molecular characterization of ascarid nematode infection (Toxascaris leonina and Toxocara cati) in captive Asiatic lions (Panthera leo persica)

  • Rahul Mohanchandra Pawar
  • Uthandaraman Lakshmikantan
  • Shakir Hasan
  • Anantula Poornachandar
  • Sisinthy Shivaji
Article

Abstract

The objective of this study was to investigate the ascarid infection in Asiatic lions using scat samples, based on microscopic analysis, PCR amplification of the ITS-2 region of ribosomal DNA and sequence analysis of the amplicons. Microscopic analysis indicated the presence of eggs of Toxascaris leonina in eleven of the sixteen scat samples analysed and in one of these eleven scats eggs of Toxocara cati were also detected. In five of the scats eggs were not detectable. The presence of T. leonina in all the infected samples was also confirmed by PCR amplification of the ITS-2 of ribosomal RNA gene and five of these also showed amplicons corresponding to T. cati, respectively. Toxocara canis infection was not observed in any of the scat samples. Nucleotide sequence analysis of the ITS-2 region indicated 97% to 99% similarity with T. leonina and T. cati, respectively. To our knowledge, this is the first molecular characterization of ascarid infection in captive Asiatic lions from a zoological garden of India. This study also indicates that Asiatic lions are more prone to infection either with T. leonina or T. cati and the parasite is not host specific.

Keywords

Ascarid nematodes Asiatic lion ITS-2 Toxascaris leonina Toxocara cati 

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References

  1. Bjork K.E., Averbeck G.A., Stromberg B.E. 2000. Parasites and parasite stages of free-ranging wild lions (Panthera leo) of northern Tanzania. Journal of Zoo and Wildlife Medicine, 31, 56–61. DOI: 10.1638/1042-7260(2000)031[0056:PAPSOF] 2.0.CO;2.PubMedGoogle Scholar
  2. Blair D., Campos A., Cummings M.P., Laclette J.P. 1996. Evolutionary biology of the parasitic platyhelminths: the role of molecular phylogenetics. Parasitology Today, 12, 66–71. DOI: 10.1016/0169-4758(96)80657-0.PubMedCrossRefGoogle Scholar
  3. Bowles J., Blair D., McManus D.P. 1995. A molecular phylogeny of the genus Echinococcus. Parasitology, 110, 317–328. DOI: 10.1017/S0031182000080902.PubMedCrossRefGoogle Scholar
  4. Campbell A.J.D., Gasser R.B., Chilton N.B. 1995. Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs. International Journal for Parasitology, 25, 359–365. DOI: 10.1016/0020-7519(94)00116-6.PubMedCrossRefGoogle Scholar
  5. Despommier D. 2003. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiological Reviews, 16, 265–272. DOI: 10.1128/CMR.16.2.265-272.2003.CrossRefGoogle Scholar
  6. Fisher M. 2003. Toxocara cati: an underestimated zoonotic agent. Trends in Parasitology, 19, 167–170. DOI: 10.1016/S1471-4922(03)00027-8.PubMedCrossRefGoogle Scholar
  7. Gibbons L.M., Jacobs D.E., Sani R.A. 2001. Toxocara malaysiensis n. sp. (Nematoda: Ascaridoidea) from the domestic cat (Felis catus Linnaeus, 1758). Journal of Parasitology, 87, 660–665. DOI: 10.1645/0022-3395 (2001)087[0660:TMNSNA]2.0.CO;2.PubMedCrossRefGoogle Scholar
  8. Hoste H., Chilton N.B., Gasser R.B., Beveridge I. 1995. Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae). International Journal for Parasitology, 25, 75–80. DOI: 10.1016/0020-7519(94)00085-3.PubMedCrossRefGoogle Scholar
  9. Ito S. 1980. Modified Winsconsin sugar centrifugal flotation technique for nematode eggs in bovine faeces. Journal of the Japanese Veterinary Medicine Association, 33, 424–429.Google Scholar
  10. Jacobs D.E., Zhu X., Gasser R.B., Chilton N.B. 1997. PCR-based methods for identification of potentially zoonotic ascaridoid parasites of the dog, fox and cat. Acta Tropica, 68, 191–200. DOI: 10.1016/S0001-706X(97)00093-4.PubMedCrossRefGoogle Scholar
  11. Kumar S., Dudley J., Nei M., Tamura K. 2008. MEGA: A biologistcentric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics, 9, 299–306. DOI: 10.1093/bib/bbn017.PubMedCrossRefGoogle Scholar
  12. Labarthe N., Serrao M.L., Ferreira A.M., Almeida N.K., Guerrero J. 2004. A survey of gastrointestinal helminths in cats of the metropolitan region of Rio de Janeiro, Brazil. Veterinary Parasitology, 123, 133–139. DOI: 10.1016/j.vetpar.2004.06.002.PubMedCrossRefGoogle Scholar
  13. Li M.-W., Zhu X.-Q., Gasser R.B., Lin R.-Q., Sani R.A., Lun Z.-R., Jacobs D.E. 2006. The occurrence of Toxocara malaysiensis in cats in China, confirmed by sequence-based analyses of ribosomal DNA. Parasitology Research, 99, 554–557. DOI: 10.1007/s00436-006-0194-z.PubMedCrossRefGoogle Scholar
  14. Martinez-Barbabosa I., Vazquez Tsuji O., Cabello R.R., Cardenas E.M., Chasin O.A. 2003. The prevalence of Toxocara cati in domestic cats in Mexico City. Veterinary Parasitology, 114, 43–49. DOI: 10.1016/S0304-4017(03)00038-4.PubMedCrossRefGoogle Scholar
  15. Minnaar W.N., Krecek R.C., Fourie L.J. 2002. Helminths in dogs from a periurban resource-limited community in Free State Province, South Africa. Veterinary Parasitology, 107, 343–349. DOI: 10.1016/S0304-4017(02)00155-3.PubMedCrossRefGoogle Scholar
  16. Nashiruddullah N., Chakraborty A. 2001. Parasites of captive wild carnivores of Assam State Zoo. Intas Polivet, 2, 173–181.Google Scholar
  17. Oliveira-Sequeira T.C., Amarante A.F., Ferrari T.B., Nunes L.C. 2002. Prevalence of intestinal parasites in dogs from São Paulo State, Brazil. Veterinary Parasitology, 103, 19–27. DOI: 10.1016/S0304-4017(01)00575-1.PubMedCrossRefGoogle Scholar
  18. Opara M.N., Osuji C.T., Opara J.A. 2010. Gastrointestinal parasitism in captive animals at the zoological garden, Nekede Owerri, South-east Nigeria. Report and Opinion, 2, 21–28.Google Scholar
  19. Patnaik M.M., Acharjyo L.N. 1970. Notes on the helminth parasites of vertebrates in Baranga Zoo (Orissa). Indian Veterinary Journal, 47, 723–730.PubMedGoogle Scholar
  20. Patton S., Rabinowitz A.R. 1994. Parasites of wild Felidae in Thailand: a coprological survey. Journal of Wildlife Diseases, 30, 472–475.PubMedGoogle Scholar
  21. Prescott C.W. 1981. Fenbendazole in the treatment of intestinal parasites of circus lions and tigers. Veterinary Record, 109, 15–16. DOI: 10.1136/vr.109.1.15.PubMedCrossRefGoogle Scholar
  22. Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory Press, New York.Google Scholar
  23. Schantz P.M. 1991. Parasitic zoonoses in perspective. International Journal for Parasitology, 21, 161–170.PubMedCrossRefGoogle Scholar
  24. Stevenson L.A., Chilton N.B., Gasser R.B. 1995. Differentiation of Haemonchus placei from H. contortus (Nematoda: Trichostrongylidae) by the ribosomal second internal transcribed spacer. International Journal for Parasitology, 25, 483–488. DOI: 10.1016/0020-7519(94)00156-I.PubMedCrossRefGoogle Scholar
  25. Taylor M.R. 2001. The epidemiology of ocular toxocariasis. Journal of Helminthology, 75, 109–118. DOI: 10.1079/JOH200175.PubMedGoogle Scholar
  26. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882. DOI: 10.1093/nar/25.24.4876.PubMedCrossRefGoogle Scholar
  27. Urquhart G.M., Armour J., Duncan J.L., Dunn A.M., Jennings F.W. 1996. Veterinary Parasitology., 2nd ed., Blackwell, Oxford.Google Scholar
  28. Van Knapen F., Buijs J. 1993. Diagnosis of Toxocara infection. In: (Eds. J.W. Lewis and R.M. Maizels) Toxocara and toxocariasis: clinical, epidemiological and molecular perspectives. Institute of Biology, London, pp. 49–54.Google Scholar
  29. Žąsitytė R., Grikienienė J. 2002. Some data on endoparasites of common mole in Lithuania. Acta Zoologica Lituanica, 12(4), 403–409.Google Scholar
  30. Zhu X.Q., Jacobs D.E., Chilton N.B., Sani R.A., Cheng N.A.B.Y., Gasser R.B. 1998. Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia. Parasitology, 117, 155–164.PubMedCrossRefGoogle Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Rahul Mohanchandra Pawar
    • 1
  • Uthandaraman Lakshmikantan
    • 1
  • Shakir Hasan
    • 1
  • Anantula Poornachandar
    • 1
  • Sisinthy Shivaji
    • 1
  1. 1.Laboratory for Conservation of Endangered SpeciesCentre for Cellular and Molecular BiologyHyderabadIndia

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