Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation
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The lectin binding properties of Fasciola hepatica miracidia were studied by a panel of fluorescein- and gold-conjugated lectins (ConA, LCA, WGA, LEA, SBA, HPA and UEA-I). The presence of mannose and/or glucose residues was demonstrated with ConA and LCA as weak diffuse fluorescence of the miracidial surface, which was more intense at the anterior part of the larva. The N-acetylglucosamine-binding lectins WGA and LEA reacted intensely with the whole miracidial surface. No labelling with N-acetylgalactosamine and/or galactose-specific (SBA and HPA) and fucose-specific UEA-I lectins was observed. The possibility that the specific recognition of the miracidial surface carbohydrates by lectins may initiate the process of transformation of the miracidia into sporocysts was examined in vitro in physiological saline for Galba truncatula. Incubation in the presence of ConA and WGA resulted in facilitation of the transformation process. Facilitation was absent in the presence of inhibitor sugars. Incubation in the presence of SBA or UEA-I had no effect. The results suggested a possible impact of carbohydrate-lectin interactions in transformation of miracidia of F. hepatica to sporocysts in vivo.
KeywordsFasciola hepatica miracidia lectin binding lectin-carbohydrate interactions in vitro miracidium-to-sporocyst transformation
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- Daniel B.E., Preston T.M., Southgate V.R. 1992. The in vitro transformation of the miracidium to the mother sporocyst of Schistosoma margrebowiei; changes in the parasite surface and implications for interactions with snail plasma factors. Parasitology, 104, 41–49. DOI:10.1017/S0031182000060789.PubMedCrossRefGoogle Scholar
- Georgieva K., Yoneva A., Popov I., Mizinska-Boevska Y., Stoitsova S. 2005. Lectin-binding properties of the surface of Fasciola hepatica sporocysts. Comptes Rendus de l’Académie Bulgare des Sciences, 58, 973–976.Google Scholar
- Georgieva K., Yoneva A., Mizinska-Boevska Y. 2007. Lectin binding characteristics of Fasciola hepatica rediae. Comptes Rendus de l’Académie Bulgare des Sciences, 60, 315–318.Google Scholar
- Horák P., van der Knaap W.P.W. 1997. Lectins in snail-trematode immune interactions: a review. Folia Parasitologica, 44, 161–172.Google Scholar
- Iguchi S.M.M., Momoi T., Egawa K., Matsumoto J.J. 1985. An Nacetylneuraminic acid-specific lectin from the body surface mucus of African giant snail. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 85,4, 897–900. DOI: 10.1016/0305-0491(85)90085-9.Google Scholar
- Loker E.S. 2010. Gastropod immunobiology. In: (Ed. K. Söderhäll) Invertebrate Immunity. Landes Bioscience and Springer Science + Business Media, http://www.ncbi.nlm.nih.gov/books/NBK45994/.
- Pales Espinosa E., Perrigault M., Ward J.E., Shumway S.E., Allam B. 2009. Lectins associated with the feeding organs of the oys ter Crassostrea virginica can mediate particle selection. Biological Bulletin, 217, 130–141.Google Scholar
- Peterson N.A., Hokke C.H., Deelder A.M., Yoshino T.P. 2009. Glycotope analysis in miracidia and primary sporocysts of Schistosoma mansoni: differential expression during the miracidium-to-sporocyst transformation. International Journal for Parasitology, 39, 1331–1344. DOI: 10.1016/j.ijpara.2009.06.002.PubMedCrossRefGoogle Scholar
- Stoitsova S., Polyakova-Krusteva O., Vinarova M. 1991. Fasciola hepatica: a cytochemical study of tegumental blebs, apical vesicles and infolds and their possible relation to surface membrane shedding. Helminthologia, 28, 5–11.Google Scholar