Acta Parasitologica

, Volume 57, Issue 1, pp 1–6 | Cite as

Preliminary experiments on use of zebrafish as a laboratory model for Giardia duodenalis infection

  • Kristoffer R. Tysnes
  • Anders Jørgensen
  • Trygve Poppe
  • Paul J. Midtlyng
  • Lucy J. Robertson
Article
  • 134 Downloads

Abstract

Although Giardia duodenalis is considered a parasite of mammals, different genotypes have been identified as infecting several species of freshwater and marine fish in Australia. Establishment of G. duodenalis infection in common laboratory zebrafish (Danio rerio), could provide an excellent tool for a range of studies on Giardia. We conducted preliminary experiments to investigate this possibility. Zebrafish were inoculated with viable G. duodenalis cysts from two different Assemblages (A and D) using a modified oro-gastric tube. Direct microscopy and immunofluorescent antibody test were used to check for Giardia cysts/trophozoites in the intestine, and histology was performed on intestinal mucosa to evaluate possible pathological changes. Giardia cysts were successfully deposited in the zebrafish alimentary tract using a modified oro-gastric tube, and were maintained in the fish gut for at least 8 days. Although a single trophozoite was observed in one fish three days post-exposure, we were unable to demonstrate established, propagative infection under the conditions tested.

Keywords

Animal model cyst Giardia infection trophozoite zebrafish 

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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  • Kristoffer R. Tysnes
    • 1
  • Anders Jørgensen
    • 2
  • Trygve Poppe
    • 3
  • Paul J. Midtlyng
    • 3
  • Lucy J. Robertson
    • 1
  1. 1.Parasitology Laboratory, Section of Microbiology, Immunology and Parasitology, Department of Food Safety and Infection BiologyNorwegian School of Veterinary ScienceOsloNorway
  2. 2.Section of ParasitologyNorwegian Veterinary InstituteOsloNorway
  3. 3.Department of Basic Sciences and Aquatic MedicineNorwegian School of Veterinary ScienceOsloNorway

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