Acta Parasitologica

, Volume 56, Issue 1, pp 40–47 | Cite as

Ultrastructural and cytochemical studies of GER-bodies in the intrauterine eggs of Wenyonia virilis Woodland, 1923 (Cestoda, Caryophyllidea)

  • Daniel Młocicki
  • Zdzisław Świderski
  • John S. Mackiewicz
  • Mohammed H. Ibraheem
Article

Abstract

Ultrastructural and cytochemical characteristics of GER-bodies observed in the vitellocyte cytoplasm of the intrauterine eggs of the caryophyllidean cestode Wenyonia virilis are described. In this species GER-bodies were observed only in the cytoplasm of vitellocytes, surrounded by a newly formed egg-shell. They are composed of spherical areas of condensed, electron-dense cytoplasm which contains concentrically arranged parallel lamellae of granular endoplasmic reticulum (GER), forming characteristic balls of different sizes. Each GER-body is surrounded by numerous free ribosomes, polyribosomes, α-glycogen rosettes and large mitochondria. Results of cytochemical analysis by means of PATSC-SP test for polysaccharides indicated that glycogen is absent within the GER-bodies, however, a strongly positive reaction was observed only in large aggregations of α-glycogen rosettes and β-glycogen particles, localised usually near GER-bodies.

Keywords

Wenyonia virilis Caryophyllidea cytochemistry endoplasmic reticulum GER-bodies ultrastructure 

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References

  1. Björkman N., Thorsell W. 1963. On the fine morphology of the formation of the egg-shell globules in vitelline glands of the liver fluke (Fasciola hepatica L.). Experimental Cell Research, 39, 18–28. DOI: 10.1016/0014-4827(63)90077-6.Google Scholar
  2. Bruňanská M., DrobnÍková P., Oros M. 2009. Vitellogenesis in the cestode Atractolytocestus huronensis Anthony, 1958 (Caryophyllidea: Lytocestidae). Parasitology Research, 105, 647–654. DOI: 10.1007/s00436-009-1436-7.CrossRefPubMedGoogle Scholar
  3. Bruňanská M., Poddubnaya L.G., Dezfuli B.S. 2005. Vitellogenesis in two spathebothriidean cestodes. Parasitology Research, 96, 390–397.CrossRefPubMedGoogle Scholar
  4. Erasmus D.A. 1973. A comparative study of the reproductive system of mature, immature and “unisexual” female Schistosoma mansoni. Parasitology, 67, 165–183. DOI: 10.1017/S0031182000046394.CrossRefPubMedGoogle Scholar
  5. Erasmus D.A. 1975. Schistosoma mansoni, development of the vitelline cells, its role in drug sequestration, and changes induced by Astiban. Experimental Parasitology, 38, 240–256. DOI: 10.1016/0014-4894(75)90027-2.CrossRefPubMedGoogle Scholar
  6. Halton D.W., Stranock S.D., Hardcastle A. 1974. Vitelline cell development in monogenean parasites. Zeitschrift für Parasitenkunde, 45, 45–61. DOI: 10.1007/BF00636526.CrossRefPubMedGoogle Scholar
  7. Hirschberg C.B., Snider M.D. 1987. Topography of glycosylation in the rough endoplasmic reticulum and Golgi apparatus. Annual Review of Biochemistry, 56, 915–944. DOI: 10.1146/annurev.bi.56.070187.000431.CrossRefGoogle Scholar
  8. Irwin S.W., Threadgold L.T. 1970. Electron-microscope studies on Fasciola hepatica VIII. The development of the vitelline cells. Experimental Parasitology, 28, 399–411. DOI: 10.1016/0014-4894(70)90108-6.CrossRefPubMedGoogle Scholar
  9. Kendrew J. 1994. The encyclopedia of molecular biology. Blackwell Science, Oxford, UK, 1165 pp.CrossRefGoogle Scholar
  10. Mackiewicz J.S. 1972. Caryophyllidea (Cestoidea): a review. Experimental Parasitology, 31, 417–512. DOI: 10.1016/0014-4894(72)90103-8.CrossRefPubMedGoogle Scholar
  11. Meepool A., Wanichanon C., Viyanant V., Sobhon P. 2006. Development and roles of vitelline cells in eggshell formation in Fasciola gigantica. Invertebrate Reproduction and Development, 49, 9–17.CrossRefGoogle Scholar
  12. Młocicki D., Świderski Z., Mackiewicz J.S., Ibraheem M.H. 2010a. Ultrastructure of intrauterine eggs: Evidence of early ovoviviparity in the caryophyllidean cestode Wenyonia virilis Woodland, 1923. Acta Parasitologica, 55, 349–358. DOI: 10.2478/S11686-010-0044-0.CrossRefGoogle Scholar
  13. Młocicki D., Swiderski Z., Conn D.B. 2010b. Ultrastructure of the early embryonic development of Corallobothrium fimbriatum (Cestoda: Proteocephalidea). Journal of Parasitology, 96, 839–846. DOI: 10.1645/GE-24381.1.CrossRefPubMedGoogle Scholar
  14. Oros M., Hanzelova V., Scholz T., Mackiewicz J.S. 2008. Phylogenetic relationships of the monozoic tapeworms (Eucestoda: Caryophyllidea) inferred from morphological characters. Systematic Parasitology, 70, 1–14. DOI: 10.1007/s11230-008-9133-y.CrossRefPubMedGoogle Scholar
  15. Poddubnaya L.G., Gibson D.I., Świderski Z., Olson P.D. 2006. Vitellocyte ultrastructure in the cestode Didymobothrium rudolphii (Monticelli, 1890): possible evidence for the recognition of divergent taxa within the Spathebothriidea. Acta Parasitologica, 51, 255–263. DOI: 10.2478/s11686-006-0039-z.CrossRefGoogle Scholar
  16. Schmidt J. 1998. Glycan vesicle formation in vitellocytes and hatching vacuoles in eggs of Echinostoma caproni and Fasciola hepatica (Digenea). Tissue & Cell, 30, 416–426. DOI: 10.1016/S0040-8166(98)80056-6.CrossRefGoogle Scholar
  17. Stitt A.W., Fairweather I. 1996. Fasciola hepatica: disruption of the vitelline cells in vitro by the sulphoxide metabolite of triclabendazole. Parasitology Reseach, 82, 333–339. DOI: 10.1007/s004360050122.CrossRefGoogle Scholar
  18. Świderski Z. 1985. Embryonic development of Schistosoma mansoni and Schistosoma haematobium: egg envelope formation. South African Journal of Sciences, 81, 48.Google Scholar
  19. Świderski Z. 1986. Blastomere differentiation during embryonic development of Schistosoma mansoni. In: Proceedings of the 11th International Congress on Electron Microscopy, August 31–September 7, Kyoto, Japan, 3321–3322.Google Scholar
  20. Świderski Z. 1994a. Origin, differentiation and ultrastructure of egg envelopes surrounding the miracidia of Schistosoma mansoni. Acta Parasitologica, 39, 64–72.Google Scholar
  21. Świderski Z. 1994b. Origin, differentiation and ultrastructure of egg envelopes surrounding the coracidia of Bothriocephalus clavibothrium (Cestoda, Pseudophyllidea). Acta Parasitologica, 39, 73–81.Google Scholar
  22. Świderski Z. 1994c. Homology and analogy in egg envelopes surrounding the coracidia of Bothriocephalus clavibothrium and miracidia of Schistosoma mansoni. Acta Parasitologica, 39, 123–130.Google Scholar
  23. Świderski Z., Bruňanská M., Poddubnaya L.G. 2004a. Ultrastructural and cytochemical studies on vitellogenesis in caryophyllidean cestode Caryophyllaeus laticeps. Proceedings of the 9th European Multicolloquium of Parasitology, Valencia, Spain, 18–23 July, 2004, 602.Google Scholar
  24. Świderski Z., Bruňanská M., Poddubnaya L.G., Mackiewicz J.S. 2004b. Ultrastructural and cytochemical studies on vitellogenesis in caryophyllidean cestode Khawia armeniaca (Cholodkovski, 1915). Acta Parasitologica, 49, 16–24.Google Scholar
  25. Świderski Z., Mackiewicz J.S. 1976. Electron microscope study of vitellogenesis in Glaridacris catostomi (Cestoidea: Caryophyllidea). International Journal for Parasitology, 6, 61–73. DOI: 10.1016/0020-7519(76)90011-4.CrossRefPubMedGoogle Scholar
  26. Świderski Z., Młocicki D., Mackiewicz J.S., Miquel J., Ibraheem M.H., Brunanská M. 2009. Ultrastructure and cytochemistry of vitellogenesis in Wenyonia virilis Woodland, 1923 (Cestoda, Caryophyllidea). Acta Parasitologica, 54, 131–142. DOI: 10.2478/s11686-009-0028-0.CrossRefGoogle Scholar
  27. Świderski Z., Xylander W.E.R. 1998. Types of vitellocytes and vitellogenesis in the Cestoda in relation to different types of embryonic development, ovoviviparity and life cycle. Wiadomości Parazytologiczne, 44, 604.Google Scholar
  28. Świderski Z., Xylander W.E.R. 2000. Vitellocytes and vitellogenesis in cestodes in relation to development, egg production and life cycle. International Journal for Parasitology, 30, 805–817. DOI: 10.1016/S0020-7519(00)00066-7.CrossRefPubMedGoogle Scholar
  29. Thiery J.P. 1967. Mise en evidence de polysaccharides sur coupes fines en microscopie electronique. Journal de Microscopie, Paris, 6, 987–1018.Google Scholar
  30. Threadgold L.T. 1982. Fasciola hepatica: Stereological analysis of vitelline cell development. Experimental Parasitology, 54, 952–365. DOI: 10.1016/0014-4894(82)90044-3.CrossRefGoogle Scholar
  31. Varki A., Cummings R., Esko J., Freeze H., Hart H., Marth J. 1999 (with errata January 2009). Essentials of Glycobiology. Edited by the Consortium of Glycobiology Editors, Cold Spring Harbor Laboratory Press, La Jolla, California. (available online at http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=glyco.TOC/depth=2).Google Scholar
  32. Woodland W.N.F. 1923. On some remarkable new forms of Caryophyllaeidae from the Anglo-Egyptian Sudan, and a revision of the families of the Cestodaria. Quarterly Journal of Microscopical Science (New Series), 67, 435–472.Google Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2011

Authors and Affiliations

  • Daniel Młocicki
    • 1
    • 2
  • Zdzisław Świderski
    • 1
    • 2
  • John S. Mackiewicz
    • 3
  • Mohammed H. Ibraheem
    • 4
  1. 1.W. Stefański Institute of ParasitologyPolish Academy of SciencesWarsawPoland
  2. 2.Department of General Biology and ParasitologyMedical University of WarsawWarsawPoland
  3. 3.Department of Biological SciencesState University of New York at AlbanyAlbanyUSA
  4. 4.Department of Zoology, Faculty of ScienceMinia UniversityEl-MiniaEgypt

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