Acta Parasitologica

, Volume 56, Issue 1, pp 8–13 | Cite as

Detection of Theileria annulata by the PCR-RFLP in ticks (Acari, Ixodidae) collected from cattle in West and North-West Iran

  • Mosa Tavassoli
  • Mohammad Tabatabaei
  • Bijan Esmaeil Nejad
  • Mehran Hasani Tabatabaei
  • Amin Najafabadi
  • Seyed Hassan Pourseyed


The presence of potential vectors, ticks, and susceptible hosts of bovine malignant theileriosis in all parts of Iran pose a real threat to food animal industry. The present study was conducted to determine the infection rate of ticks collected from naturally occurring bovine theileriosis in West and North-West Iran. Two hundred and thirty seven cattle suspected of suffering from theileriosis were investigated for the presence of Theileria annulata in the blood smears and any tick species on their body. In this study, 402 ticks were obtained from 99 cattle. The examination of 402 ticks by polymerase chain reaction (PCR) using primers derived from the gene encoding heat shock protein70 (Hsp70) revealed that 39.9% of Hyalomma anatolicum anatolicum, 3.5% of H. asiaticum asiaticum, and 18.2% H. anatolicum excavatum, were infected with T. annulata. The results suggest that H. a. anatolicum may play a major role in transmission of T. annulata infection in Iran. Finally, digestion of the PCR products of T. annulata with two different restriction enzymes produced only a single pattern.


Theileria annulata Hyalomma PCR-RFLP ticks Iran 


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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2011

Authors and Affiliations

  • Mosa Tavassoli
    • 1
  • Mohammad Tabatabaei
    • 2
  • Bijan Esmaeil Nejad
    • 1
  • Mehran Hasani Tabatabaei
    • 3
  • Amin Najafabadi
    • 3
  • Seyed Hassan Pourseyed
    • 1
  1. 1.Department of Pathobiology, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  2. 2.Department of Microbiology, Faculty of Veterinary MedicineShiraz UniversityShirazIran
  3. 3.Private practitionerUrmiaIran

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