Detection of Theileria annulata by the PCR-RFLP in ticks (Acari, Ixodidae) collected from cattle in West and North-West Iran
The presence of potential vectors, ticks, and susceptible hosts of bovine malignant theileriosis in all parts of Iran pose a real threat to food animal industry. The present study was conducted to determine the infection rate of ticks collected from naturally occurring bovine theileriosis in West and North-West Iran. Two hundred and thirty seven cattle suspected of suffering from theileriosis were investigated for the presence of Theileria annulata in the blood smears and any tick species on their body. In this study, 402 ticks were obtained from 99 cattle. The examination of 402 ticks by polymerase chain reaction (PCR) using primers derived from the gene encoding heat shock protein70 (Hsp70) revealed that 39.9% of Hyalomma anatolicum anatolicum, 3.5% of H. asiaticum asiaticum, and 18.2% H. anatolicum excavatum, were infected with T. annulata. The results suggest that H. a. anatolicum may play a major role in transmission of T. annulata infection in Iran. Finally, digestion of the PCR products of T. annulata with two different restriction enzymes produced only a single pattern.
KeywordsTheileria annulata Hyalomma PCR-RFLP ticks Iran
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- Bhattacharyulu Y., Chaudhri R.P., Gill B.S. 1975. Studies on the development of Theileria annulata (Dschunskowsky and Luhs, 1904) in the tick — Hyalomma anatolicum anatolicum (Koch, 1844). Annales de Parasitologie Humaine et Comparee (Paris), 50, 397–408.Google Scholar
- Dumanli N., Aktas M., Cetinkaya B., Cakmak A., Koroglu E., Saki C.E., Erdogmus Z., Nalbantoglu S., Ongor H., Simşek S., Karahan M., Altay K. 2005. Prevalence and distribution of tropical theileriosis in eastern Turkey. Veterinary Parasitology, 127, 9–15. DOI: 10.1016/j.vetpar.2004.08.006.CrossRefPubMedGoogle Scholar
- Estrada-Pena A., Bouattour A., Camicas J.L., Walker A.R. 2004. Ticks of domestic animals in the Mediterranean region: a guide to identification of species. University of Zaragoza, Spain, 43–131.Google Scholar
- Gautam O.P., Dhar S. 1983. Bovine tropical theileriosis — a review. 1. Prevalence, transmission and symptoms. Tropical Veterinary Animal Science Research, 1, 1–18.Google Scholar
- Hoffman G., Hörcher F., Schein E., Gerber H. 1971. Seasonal occurrence of ticks and piroplasm in domestic animals in the Asiatic provinces of Turkey. Berliner und Münchener Tierärztliche Wochenschrift, 84, 152–156.Google Scholar
- Hoogstraal H., Kaiser M.N. 1959. Observation on Egyptian a Hyalomma ticks (Ixodoidae, Ixodidae). 5. Biological notes and differences in identity of Hyalomma anatolicum and its subspecies anatolicum Koch and excavatum Koch among Russian and other workers. Identity of H. lusitanicum Koch. Annals of the Entomological Society of America, 52, 243–261.Google Scholar
- Jensen K., Paxton E., Waddington D., Talbot R., Darghouth M.A., Glass E.J. 2008. Differences in the transcriptional responses induce by Theileria annulata infection in bovine monocytes derived from resistant and susceptible cattle breeds. International Journal for Parasitology, 38, 313–325. DOI: 10.1016/j.ijpara.2007.08.007.CrossRefPubMedGoogle Scholar
- Mazlum Z. 1971. Different ticks occurring in Iran (geographical distribution, seasonal activities, hosts). Faculty Veterinary Medicine of Tehran, Iran, 27, 1–32.Google Scholar
- Mehlhorn H., Schein E., Ahmed J.S. 1994. Theileria. In: (Ed. J.P. Kreier) Parasitic Protozoa. Vol. 7. Academic Press, San Diego, 217–304.Google Scholar
- Sayin F., Karaer Z., Dincer S., Cakmak A., Inci A., Yukari B.A., Eren H., Vatansever Z., Nalbantoglu S., Melrose T.R. 2003.A comparison of susceptibilities to infection of four species of Hyalomma ticks with Theileria annulata. Veterinary Parasitology, 113, 115–121. DOI: 10.1016/S0304-4017(03)000 45-1.CrossRefPubMedGoogle Scholar
- Schein E., Rehbein G., Voigt W.P., Zweygarth E. 1981. Babesia equi (Laveran, 1901) development in horses and in lymphocyte culture. Zeitschrift für Tropenmedizin und Parasitologie, 32, 223–227.Google Scholar
- Telmadarraiy Z., Bahrami A., Vatandoost H. 2004. A Survey on Fauna of Ticks in West Azerbaijan Province, Iran. Iranian Journal Public Health, 33, 65–69.Google Scholar