Acta Parasitologica

, Volume 55, Issue 3, pp 215–229 | Cite as

Gyrodactylids (Gyrodactylidae, Monogenea) infecting Oreochromis niloticus niloticus (L.) and O. mossambicus (Peters) (Cichlidae): A pan-global survey

  • Adriana García-Vásquez
  • Haakon Hansen
  • Kevin W. Christison
  • Miquel Rubio-Godoy
  • James E. Bron
  • Andrew P. Shinn
Article

Abstract

Gyrodactylus infections in intensively-reared populations of Nile tilapia, Oreochromis niloticus niloticus, have been associated world-wide with high mortalities of juvenile fish. In this study, 26 populations of Gyrodactylus parasitising either O. n. niloticus or Mozambique tilapia, Oreochromis mossambicus, were sampled from fourteen countries and compared with type material of Gyrodactylus cichlidarum Paperna, 1968, Gyrodactylus niloticus (syn. of G. cichlidarum) and Gyrodactylus shariffi Cone, Arthur et Bondad-Reantaso, 1995. Representative specimens from each population were bisected, each half being used for morphological and molecular analyses. Principal component analyses (PCA) identified five distinct clusters: (1) a cluster representing G. cichlidarum collected from O. n. niloticus from 13 countries; (2) the G. shariffi paratype; (3) three specimens with pronounced ventral bar processes collected from two populations of Mexican O. n. niloticus (Gyrodactylus sp. 1); (4) four specimens collected from an Ethiopian population nominally identified as O. n. niloticus (Gyrodactylus sp. 2); (5) nine gyrodactylids from South African O. mossambicus (Gyrodactylus sp. 3). Molecular analyses comparing the sequence of the ribosomal transcribed spacer regions (ITS 1 and 2) and the 5.8S gene from the non-hook bearing half of worms representative for each population and for each cluster of parasites, confirmed the presence of G. cichlidarum in most samples analysed. Molecular data also confirmed that the DNA sequence of Gyrodactylus sp. 2 and Gyrodactylus sp. 3 (the morphologically-cryptic group of South African specimens from O. mossambicus) differed from that of G. cichlidarum and therefore represent new species; no sequences were obtained from Gyrodactylus sp. 1. The current study demonstrates that G. cichlidarum is the dominant species infecting O. n. niloticus, being found in 13 of the 15 countries sampled.

Keywords

Monogenea Gyrodactylus tilapia Oreochromis niloticus niloticus O. mossambicus cichlid aquaculture 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Adriana García-Vásquez
    • 1
  • Haakon Hansen
    • 2
  • Kevin W. Christison
    • 3
  • Miquel Rubio-Godoy
    • 4
  • James E. Bron
    • 1
  • Andrew P. Shinn
    • 1
  1. 1.Institute of AquacultureUniversity of StirlingStirlingUK
  2. 2.National Veterinary InstituteSection for ParasitologyOsloNorway
  3. 3.Department of Biodiversity and Conservation Biology (Zoology)University of the Western CapeBellvilleSouth Africa
  4. 4.Instituto de Ecología, A.C., Red de Biología EvolutivaKm 2.5 Ant. Carretera a CoatepecXalapa, VeracruzMéxico

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