Acta Parasitologica

, Volume 55, Issue 3, pp 245–253 | Cite as

Ultrastructural studies on egg envelopes surrounding the miracidia of Mediogonimus jourdanei Mas-Coma et Rocamora, 1978 (Digenea, Microphalloidea, Prosthogonimidae)

  • Zdzisław Świderski
  • Abdoulaye J. S. Bakhoum
  • Daniel Młocicki
  • Jordi Miquel
Article

Abstract

The intrauterine, mature and fully embryonated eggs of the prosthogonimid trematode Mediogonimus jourdanei Mas-Coma et Rocamora, 1978 were examined by means of transmission electron microscopy (TEM), using high pressure freezing, freeze substitution and infiltration with resin techniques. Each embryonated egg is composed of a miracidium surrounded by three envelopes: (1) the egg shell, (2) the outer and (3) inner envelopes. Egg envelopes play an important role in the protection, metabolism, storage of nutritive reserves and the general biology of the M. jourdanei egg. The inner envelope is characterised by large, flattened nuclei, and its syncytial cytoplasm contains a heavy accumulation of glycogen, lipid droplets, mitochondria and large vesicles. These traits indicate that this layer has the features of a metabolically-active syncytial layer with an energy storage capability. In the infective eggs observed before the hatching of the miracidium, areas of so-called “focal cytoplasmic degradation” were frequently observed, which may be involved in the autolytic process of all components of this envelopes.

Keywords

Trematoda Prosthogonimidae Mediogonimus jourdanei egg envelopes ultrastructure 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Eklu-Natey D.T., Świderski Z., Huggel H., Striebel H. 1982a. Schistosoma haematobium: egg-shell formation. Proceedings of the 11th International Congress on Electron Microscopy, August 17–24, 1982, Hamburg, Germany, 2, 605–606.Google Scholar
  2. Eklu-Natey D.T., Świderski Z., Moczoń T., Striebel H.P., Huggel H. 1982b. Ultrastructure and histochemistry of egg-shell formation in Schistosoma haematobium. Molecular and Biochemical Parasitology, Supplement, 708.Google Scholar
  3. Cribb T.H., Spratt D.M. 1990. Coryphogonimus aglaos n. g., n. sp., (Digenea: Prosthogonimidae) from the Australian bush rat, Rattus fuscipes (Rodentia: Muridae), with notes on other prosthogonimids from Australian mammals. Systematic Parasitology, 16, 77–83. DOI: 10.1007/BF00009602.CrossRefGoogle Scholar
  4. Jones A. 2008. Family Prosthogonimidae Lühe, 1909. In: (Eds. R.A. Bray, D.I. Gibson and A. Jones) Keys to the Trematoda. Vol. 3. CABI International and Natural History Museum, Wallingford, 577–590.Google Scholar
  5. Jones M.K., Sze How Bong, Green K.M., Holmes P., Mary Duke M., Lukas A., McManus D.P. 2008. Correlative and dynamic imaging of the hatching biology of Schistosoma japonicum from eggs prepared by high pressure freezing. Plos Neglected Tropical Diseases, 2(11), e.334. DOI: 10.1371/journal.pntd.0000334.g005.CrossRefGoogle Scholar
  6. Świderski Z., Moser P., Eklu-Natey D.T. 1980. The fine structure of protective envelopes of the egg of Schistosoma mansoni. In: (Eds. P. Brederoo and W. De Priester) Electron Microscopy. Vol. 2. Leiden, The Netherlands, 218–219.Google Scholar
  7. Świderski Z. 1984. Embryonic development of Schistosoma mansoni. South African Journal of Science, 80, 434.Google Scholar
  8. Świderski Z. 1985. Embryonic development of Schistosoma mansoni and S. haematobium: egg envelope formation. South African Journal of Science, 81, 43–44.Google Scholar
  9. Świderski Z. 1986. Schistosoma mattheei: egg-shell degeneration in the liver of Praomys (Mastomys) natalensis during chronic infection. In: (Eds. T. Imura, S. Maruse and T. Suzuki) Proceedings of the 11th International Congress on Electron Microscopy, August 31–September 7, 1986, Kyoto, Japan, 3567–3568.Google Scholar
  10. Świderski Z. 1988. Ultrastructure of schistosome eggs. Proceedings of the 4th Asia Pacific Conference and Workshop on Electron Microscopy, July 26–August 4, 1988, Bangkok, Thailand, 555–556.Google Scholar
  11. Świderski Z. 1993. Functional ultrastructure and origin of egg envelopes surrounding coracidia of Bothriocephalus clavibothrium (Cestoda, Pseudophyllidea). Proceedings, International 5th Beijing Conference and Exhibition on Instrumental Analysis, October 9–12, 1993, Beijing, China, 125–126.Google Scholar
  12. Świderski Z. 1994. Origin, differentiation and ultrastructure of egg envelopes surrounding the miracidia of Schistosoma mansoni. Acta Parasitologica, 39, 64–72.Google Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Zdzisław Świderski
    • 1
    • 2
  • Abdoulaye J. S. Bakhoum
    • 3
    • 4
  • Daniel Młocicki
    • 1
    • 5
  • Jordi Miquel
    • 3
    • 4
  1. 1.W. Stefański Institute of ParasitologyPolish Academy of SciencesWarsawPoland
  2. 2.Department of General Biology and ParasitologyMedical University of WarsawWarsawPoland
  3. 3.Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain
  4. 4.Institut de Recerca de la Biodiversitat, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain
  5. 5.Department of Medical BiologyMedical University of WarsawWarsawPoland

Personalised recommendations