Acta Parasitologica

, Volume 55, Issue 1, pp 58–65 | Cite as

Spermiogenesis and spermatozoon of the tapeworm Parabothriocephalus gracilis (Bothriocephalidea): Ultrastructural and cytochemical studies

  • Lenka Šípková
  • Céline Levron
  • Mark Freeman
  • Tomáš Scholz
Article

Abstract

Spermiogenesis and spermatozoon ultrastructure of the tapeworm Parabothriocephalus gracilis were described using transmission electron microscopy (TEM). Spermiogenesis is characterized by the formation of a zone of differentiation with two centrioles associated with striated rootlets, and an intercentriolar body between them. The two flagella undergo a rotation of 90° until they become parallel to the median cytoplasmic extension with which they fuse. Electron-dense material is present in the apical region of the zone of differentiation in the early stages of spermiogenesis. This electron-dense material is characteristic for the orders Bothriocephalidea and Diphyllobothriidea. The mature spermatozoon contains two axonemes of the 9 + ‘1’ trepaxonematan pattern, nucleus, parallel cortical microtubules and electron-dense granules of glycogen. The anterior extremity of the spermatozoon exhibits a single helical electron-dense crested body 130 nm thick. One of the most interesting features is the presence of a ring of cortical microtubules surrounding the axoneme. This character has been reported only for species of the order Bothriocephalidea and may be unique in this cestode group.

Keywords

Eucestoda Bothriocephalidea Parabothriocephalus gracilis spermiogenesis spermatozoon ultrastructure 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Lenka Šípková
    • 1
    • 2
  • Céline Levron
    • 1
  • Mark Freeman
    • 3
  • Tomáš Scholz
    • 1
  1. 1.Institute of ParasitologyBiology Centre of the Academy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  2. 2.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  3. 3.Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia

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