Acta Parasitologica

, Volume 54, Issue 4, pp 335–340 | Cite as

Recombinant ferritin protects mice against challenge with Echinococcus granulosus ?

Article

Abstract

Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.

Keywords

Echinococcus granulosus rEgferritin immunoprotection vaccine 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Yana Wang
    • 1
  • Zongji Li
    • 1
  • Zhaoyu Li
    • 2
  • Yang Bo
    • 2
  • Wei Zhao
    • 1
  1. 1.Center of Scientific Technology of Ningxia Medical UniversityYinchuanNingxia Hui Autonomous Region, China
  2. 2.The Affiliated Hospital of Ningxia Medical UniversityYinchuanNingxia Hui Autonomous Region, China

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