Recombinant ferritin protects mice against challenge with Echinococcus granulosus ?
Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.
KeywordsEchinococcus granulosus rEgferritin immunoprotection vaccine
Unable to display preview. Download preview PDF.
- Eckert J., Schantz M.P., Gasser R.B., Torgerson P.R., Bessonov A.S., Movsessian S.O., Thakur A., Grimm F., Nikogossian M.A. 2001. Geographic distribution and prevalence. In: (Eds. J. Eckert, M.A. Gemmell, F.-X. Meslin and Z.S. Pawłowski) WHO/OLE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Health Organization for Animal Health. Paris, France, 100–142.Google Scholar
- Heath D.D., Jensen O., Lightowlers M.W. 2003. Progress in control of hydatidosis using vaccination — a review of formulation and delivery of the vaccine and recommendations for practical use in control programmes. Acta Tropica, 85, 133–143. DOI: 10.1016/S0001-706X(02)00219-X.CrossRefPubMedGoogle Scholar
- Lightowlers M.W., Jensen O., Fernandez E., Iriarte J.A., Woollard D.J., Gauci C.G., Jenkins D.J., Heath D.D. 1999. Vaccination trials in Australia and Argentina confirm the effectiveness of the EG95 hydatid vaccine in sheep. International Journal for Parasitology, 29, 531–534. DOI: 10.1016/S0020-7519(99)00003-X.CrossRefPubMedGoogle Scholar
- Pater C., Muller V., Harraga S., Liance M., Godot V., Carbillet J.-P., Meillet D., Römig T., Vuitton D.A. 1998. Intestinal and systemic humoral immunological events in the susceptible Balb/c mouse strain after oral administration of Echinococcus multilocularis eggs. Parasite Immunology, 20, 623–629. DOI:10.1046/j.1365-3024.1998.00195.x.CrossRefPubMedGoogle Scholar
- Riley E.M., Dixon J.B., Kelly D.F., Cox D.A. 1985. The immune response to Echinococcus granulosus: sequential histological observations of lymphoreticular and connective tissues during early murine infection. Journal of Comparative Pathology, 95, 93–104. DOI: 10.1016/0021-9975(85)90081-7.CrossRefPubMedGoogle Scholar
- Wang Y., Zhao J., Ding S., Wang J., Zhao W. 2005. Cloning and sequence analyzing of the ferritin gene from Echinococcus granulosus of China. Ningxia Medical University Journal, 27, 253–255. DOI: CNKI:SUN:XNXY.0.2005-04-000.Google Scholar