Acta Parasitologica

, Volume 54, Issue 2, pp 172–179 | Cite as

Effects of niclosamide on Oncomelania hupensis, the intermediate snail host of Schistosoma japonicum: an enzyme-histochemical study

  • Wei Wang
  • Hongjun Li
  • Yousheng Liang
  • Jianrong Dai
Article

Abstract

An enzyme-histochemical study of five enzymes, namely succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), cytochrome oxidase (CCO), cholinesterase (CHE) and nitric oxide synthase (NOS), was elucidated in the soft tissues of Oncomelania hupensis, the intermediate host snail of Schistosoma japonicum, before and after the treatment with a suspension concentrate of niclosamide (SCN). Following the treatment of SCN, a marked loss occurred in the activity of the five enzymes mentioned above. LDH and SDH showed their strongest activity in the buccal mass and muscular fibers, CCO in buccal mass and liver, CHE in pellicle and ganglia, and NOS in muscular fibers and pharyngeal canal. The results indicate that SCN impairs the activities of the enzymes influencing the transfer of neurotransmitter and energy supply in O. hupensis, ultimately leading to the loss of various physiological functions, which is considered to be a cause of death in O. hupensis.

Keywords

Oncomelania hupensis snail control schistosomiasis niclosamide enzyme-histochemistry 

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References

  1. Ben C.E., Li S.G. (Eds.). 2001. Histochemistry. People’s Medical Publishing House, Beijing, China, 361–430.Google Scholar
  2. Bennett B.C., Kruse M.N., Roppolo J.R., Flood H.D., Fraser M., De Groat W.C. 1995. Neural control of urethral outlet activity in vivo: role of nitric oxide. Journal of Urology, 153, 2004–2009. DOI: 10.1016/S0022-5347(01)67391-9.PubMedCrossRefGoogle Scholar
  3. Bredt D.S., Hwang P.M., Snyder S.H. 1990. Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature, 347, 768–770. DOI: 10.1038/347768a0.PubMedCrossRefGoogle Scholar
  4. Bredt D.S., Snyder S.H. 1992. Nitric oxide, a novel neuronal messenger. Neuron, 8, 3–11. DOI: 10.1016/0896-6273(92)90104-L.PubMedCrossRefGoogle Scholar
  5. Bult H., Boeckxtaens G.E., Pelckmans P.A., Jordaens F.H., Van Maercke Y.M., Herman A.G. 1990. Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature, 345, 346–347. DOI: 10.1038/345346a0.PubMedCrossRefGoogle Scholar
  6. Burnnet A.L., Lowenstein C.J., Bredt D.S., Chang T.S.K., Snyder S.H. 1992. Nitric oxide: a physiologic mediator of penile erection. Science, 257, 401–403. DOI: 10.1126/science.1378650.CrossRefGoogle Scholar
  7. Dai J.R., Chen C., Liang Y.S., Zhang Y.P., Zheng Y.X., Wu F. 1998. Comparison among different samples of niclosamide ethanolamine salt wettable powder in molluscicidal effect and quality. Chinese Journal of Schistosomiasis Control, 10, 86–88. DOI: XXCB.0.1998-02-006.Google Scholar
  8. Dai J.R., Wang W., Liang Y.S., Li H.J., Guan X.H., Zhu Y.C. 2008. A novel molluscicidal formulation of niclosamide. Parasitology Research, 103, 405–412. DOI: 10.1007/s00436-008-0988-2.PubMedCrossRefGoogle Scholar
  9. De Zwaan A., Wijsman T.C. 1976. Anaerobic metabolism in Bivalvia (Mollusca). Characteristics of anaerobic metabolism. Comparative Biochemistry and Physiology (Part B), 54, 313–324. DOI: 10.1016/0305-0491(76)90247-9.CrossRefGoogle Scholar
  10. Department of Diseases Control, Ministry of Health (Ed.). 2000. Handbook of Schistosomiasis Control. Shanghai Science and Technology Press, Shanghai, China, 23 pp.Google Scholar
  11. Grozdanovic Z., Bruning G., Baumgarten H.G. 1994. Nitric oxide: a novel autonomic neurotransmitter. Acta Anatomica (Basel), 150, 16–24. DOI: 10.1159/000147598.CrossRefGoogle Scholar
  12. Hao Y., Wu X.H., Zheng H., Wang L.Y., Guo J.G., Xia G., Chen Z., Zhou X.N. 2007. Schistosomiasis situation in People’s Republic of China. Chinese Journal of Schistosomiasis Control, 19, 401–404. DOI: XXCB.0.2007-06-004.Google Scholar
  13. Huang Y.X., Xi W.P., Zhang Y.P., Wu F., Hong Q.B. 2003. Evaluation on molluscicidal effectiveness of niclosamide ethanolamine salt with different methods. Chinese Journal of Schistosomiasis Control, 15, 124–128. DOI: XXCB.0.2003-02-017.Google Scholar
  14. Karnovsky M.J., Roots L. 1964. A “direct-coloring” thiocholine method for cholinesterases. Journal of Histochemistry and Cytochemistry, 12, 219–221.PubMedGoogle Scholar
  15. Li F.J. (Ed.). 1956. Anatomy and Comparative Anatomy of Oncomelania Snail. Hubei People’s Press, Wuhan, China, 52 pp.Google Scholar
  16. Liang Y.S., Dai J.R., Zhu Y.C., Zhou X.N., Zhang Y., Xiong X.K., Huang Y.X., Hang P.Y. 2001. An enzyme-histochemical study of distribution of the nitric oxide synthase in Oncomelania hupensis. Chinese Journal of Schistosomiasis Control, 13, 196–197. DOI: XXCB.0.2001-04-001.Google Scholar
  17. Liang Y.S., Xiong X.K., Song H.T., Xiao R.W., Ye J.F., Zhang J.K., Mei F.X. 1994. Histochemical and ultrastructural changes of Oncomelania snails after winter flooding in marshland of the Yangtze River. Chinese Journal of Zoology, 29, 6–9. DOI: BIRD.0.1994-03-001.Google Scholar
  18. Liang Y.S., Xiong X.K., Xiao R.W., Ye J.F., Mei F.X. 1992. Studies on the histochemistry and enzyme-histochemistry of Oncomelania snails. Chinese Journal of Schistosomiasis Control, 4, 201–205. DOI: XXCB.0.1992-04-005.Google Scholar
  19. Liu H.C., Pen D.Y., Fu Y., Huang X.B., Li S.S., Qin Z.H., Yu B.X. 1998. An analysis on cost-effectiveness of snail-eradication with niclosamide in the field. Chinese Journal of Schistosomiasis Control, 10, 129–132. DOI: XXCB.0.1998-03-000.Google Scholar
  20. Lowenstein C.J., Dinerman J.L., Snyder S.H. 1994. Nitric oxide: a physiologic messenger. Annals of Internal Medicine, 120, 227–237.PubMedGoogle Scholar
  21. Mevorach R.A., Bogaert G.A., Kogan B.A. 1994. Role of nitric oxide in fetal lower urinary tract function. Journal of Urology, 152, 510–514.PubMedGoogle Scholar
  22. Nachlas M.M., Tsou K.C., De Souza E., Cheng C.S., Seligman A.M. 1957. Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. Journal of Histochemistry and Cytochemistry, 5, 420–436.PubMedGoogle Scholar
  23. Palmer R.M., Ferrige A.G., Moncada S. 1987. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 327, 524–526. DOI: 10.1038/327524a0.PubMedCrossRefGoogle Scholar
  24. Sanders K.M., Ward S.M. 1992. Nitric oxide as a mediator of nonadrenergic noncholinergic neurotransmission. American Journal of Physiology, 262, G379–G392.PubMedGoogle Scholar
  25. Schuman E.M., Madison D.V. 1994. Nitric oxide and synaptic function. Annual Review of Neuroscience, 17, 153–183. DOI: 10.1146/annurev.ne.17.030194.001101.PubMedCrossRefGoogle Scholar
  26. Steinmann P., Keiser J., Bos R., Tanner M., Utzinger J. 2006. Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. Lancet Infectious Diseases, 6, 411–425. DOI: 10.1016/S1473-3099(06)70521-7.PubMedCrossRefGoogle Scholar
  27. Tadros T.F. 1980. Control and assessment of the physical stability of pesticide suspension concentrates. Chemistry and Industry, 18, 211–218.Google Scholar
  28. Tan P., He C.H., Zhang Y., Chang H.B., Gen H., Gong T.P., Yuan X.X., Xiong X.K. 2000. Observation of the changes of the enzymes for histochemistry in Oncomelania hupensis after being immersed in different chemicals. Chinese Journal of Zoonosis, 16, 34–37. DOI: ZRSZ.0.2000-03-012.Google Scholar
  29. Utzinger J., Zhou X.N., Chen M.G., Bergquist R. 2005. Conquering schistosomiasis in China: the long march. Acta Tropica, 96, 69–96. DOI: 10.1016/j.actatropica.2005.08.004.PubMedCrossRefGoogle Scholar
  30. Weinbach E.C., Garbus J. 1969. Mechanism of action of reagents that uncouple oxidative phosphorylation. Nature, 221, 1016–1018. DOI: 10.1038/2211016a0.PubMedCrossRefGoogle Scholar
  31. WHO/SCHISTO/92. 1992. The role of mollusciciding in schistosomiasis control. Division of Control of Tropical Diseases. Geneva, Switzerland, 107 pp.Google Scholar
  32. Yang K., Zhou X.N., Liang Y.S., Hong Q.B., Zhang Y., Sun L.P., Yang G.J., Xiong X.K. 2003. Impact of temperature on nitric oxide synthase of Oncomelania hupensis during winter. Chinese Journal of Schistosomiasis Control, 15, 93–98. DOI: XXCB.0.2003-02-005.Google Scholar
  33. Yang K., Zhou X.N., Liang Y.S., Hong Q.B., Zhao Y., Zhang Y.P., Xiong X.K. 2004. Influence of temperature change in spring on the activities of enzymes in Oncomelania hupensis. Chinese Journal of Zoonosis, 20, 515–518. DOI: ZRSZ.0.2004-06-014.Google Scholar
  34. Zhong C.S. (Ed.). 1989. Techniques for Enzyme Cytochemistry. Shanghai Medical University Press, Shanghai, China, 52 pp.Google Scholar
  35. Zhou S.L., Lin J.Y., Jiang M.S. (Ed.). 2001. Schistosome. People’s Medical Publishing House, Beijing, China, 27–47.Google Scholar
  36. Zhou X.N. (Ed.). 2005. Science on Oncomelania Snail. Science Press, Beijing, China, 94–157.Google Scholar
  37. Zhou X.N., Guo J.G., Wu X.H., Jiang Q.W., Zheng J., Dang Hui., Wang X.H., Xu J., Zhu H.Q., Wu G.L., Li Y.S., Xu X.J., Chen H.G., Wang T.P., Zhu Y.C., Qiu D.C., Dong X.Q., Zhao G.M., Zhang S.J., Zhao N.Q., Xia G., Wang L.Y., Zhang S.Q., Lin D.D., Chen M.G., Hao Y. 2007. Epidemiology of schistosomiasis in the People’s Republic of China, 2004. Emerging Infectious Diseases, 12, 1470–1476.Google Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Wei Wang
    • 1
    • 2
    • 3
    • 4
  • Hongjun Li
    • 1
    • 2
    • 3
    • 4
  • Yousheng Liang
    • 1
    • 2
    • 3
    • 4
  • Jianrong Dai
    • 1
    • 2
    • 3
    • 4
  1. 1.Jiangsu Institute of Parasitic DiseasesWuxi CityPeople’s Republic of China
  2. 2.Key Laboratory on Technology for Parasitic Disease Prevention and ControlMinistry of HealthWuxi CityPeople’s Republic of China
  3. 3.Jiangsu Provincial Key Laboratory on Molecular Biology of ParasitesWuxi CityPeople’s Republic of China
  4. 4.Jiangsu Provincial Key Subject on Parasitic DiseasesWuxi CityPeople’s Republic of China

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