Acta Parasitologica

, Volume 54, Issue 2, pp 180–185 | Cite as

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

  • Debora Pereira García-Melo
  • Javier Regidor-Cerrillo
  • Luis Miguel Ortega-Mora
  • Esther Collantes-Fernández
  • Vanessa Silvestre Ferreira de Oliveira
  • Milton Adriano Pelli de Oliveira
  • Andrea Caetano da Silva
Research Note

Abstract

Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

Keywords

Neospora caninum isolation cattle genetic characterisation pathogenic characterisation Brazil 

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Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Debora Pereira García-Melo
    • 1
  • Javier Regidor-Cerrillo
    • 2
  • Luis Miguel Ortega-Mora
    • 2
  • Esther Collantes-Fernández
    • 2
  • Vanessa Silvestre Ferreira de Oliveira
    • 1
  • Milton Adriano Pelli de Oliveira
    • 1
  • Andrea Caetano da Silva
    • 1
  1. 1.Departamento de Parasitologia/IPTSP/Universidade Federal de GoiásGoiania, GoiásBrazil
  2. 2.SALUVET, Animal Health Department, Faculty of Veterinary SciencesComplutense University of MadridMadridSpain

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