Acta Parasitologica

, 53:407 | Cite as

Wild ruminants in the area of the North-Western Poland as potential reservoir hosts of Bartonella schoenbuchensis and B. bovis

  • Małgorzata Adamska
Original Paper


The aim of this work was to examine if the game species from the north-western Poland, roe deer (Capreolus capreolus), red deer (Cervus elaphus) and wild boar (Sus scrofa), may be reservoir hosts of bacteria from the genus Bartonella, and whether the sheep tick (Ixodes ricinus) is their vector. To this end, the prevalence of Bartonella DNA in the tissues of these game species was measured, just as in sheep ticks (I. ricinus) infesting them, and ticks collected from plants in the hunting area. The prevalence of Bartonella DNA was 39% (23/59) in roe deer and 35% (7/20) in red deer. No Bartonella DNA was detected in any of the 21 wild boars. The presence of Bartonella DNAwas detected in 1.9% of ticks infesting roe deer (2/103), while no pathogen DNA was found in the 20 ticks infesting the red deer and the 3 ticks infesting wild boars, or the 200 ticks collected from plants. Amplicons of two different lengths were obtained; 198 bp, characteristic for B. bovis, and 317 bp, characteristic for B. schoenbuchensis, which were confirmed later by sequencing. The examined ruminants are probably the reservoir hosts of B. schoenbuchensis and B. bovis in the biotope of the Puszcza Wkrzańska Forest, and wild boars do not participate in the Bartonella propagation in the environment. I. ricinus is unlikely to be the main vector of Bartonella species detected in the examined roe deer and red deer; probably other bloodsucking arthropods, parasitizing wild ruminants, play this role.


Wild ruminants Ixodes ricinus Bartonella PCR 


  1. Bermond D., Boulouis H.J., Heller R., Van Laere G., Monteil H., Chomel B.B., Sander A., Dehio C., Piemont Y. 2002. Bartonella bovis Bermond et al. sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants. International Journal of Systematic and Evolutionary Microbiology, 52, 383–390. DOI: 10.1099/ijs.0.01839-0.PubMedGoogle Scholar
  2. Boulouis H.J., Chang C.C., Henn J.B., Kasten R.W., Chomel B.B. 2005. Factors associated with the rapid emergence of zoonotic Bartonella infections. Veterinary Research, 36, 383–410. DOI: 10.1051/vetres:2005009.PubMedCrossRefGoogle Scholar
  3. Bown K.J., Bennet M., Begon M. 2004. Flea-borne Bartonella grahamii and Bartonella taylorii in bank voles. Emerging Infectious Diseases, 10, 684–687.PubMedGoogle Scholar
  4. Breitschwerdt E.B., Sontakke S., Cannedy A., Hancock S.I., Bradley J.M. 2001. Infection with Bartonella weissii and detection of Nanobacterium antigens in a North Carolina beef herd. Journal of Clinical Microbiology, 39, 879–882. DOI: 10.1128/JCM.39.3.879-882.2001.PubMedCrossRefGoogle Scholar
  5. Chang C.C., Chomel B.B., Kasten R.W., Heller R., Kocan K.M., Ueno H., Yamamoto K., Bleich V.C., Pierce B.M., Gonzales B.J., Swift P.K., Boyce W.M., Jang S.S., Boulouis H.J., Piemont Y. 2000. Bartonella spp. isolated from wild and domestic ruminants in North America. Emerging Infectious Diseases, 6, 306–311.PubMedCrossRefGoogle Scholar
  6. Chang C.C., Chomel B.B., Kasten R.W., Romano V., Tietze N. 2001. Molecular evidence of Bartonella spp. in questing adult Ixodes pacificus tick in California. Journal of Clinical Microbiology, 39, 1221–1226. DOI: 10.1128/JCM.39.4.1221-1226.2001.PubMedCrossRefGoogle Scholar
  7. Dehio C., Lanz C., Pohl R., Behrens P., Bermond D., Piemont Y., Sander A. 2001. Bartonella schoenbuchii sp. nov., isolated from the blood of deer. International Journal of Systematic and Evolutionary Microbiology, 51, 1557–1565.PubMedGoogle Scholar
  8. Dehio C., Sauder U., Hiestand R. 2004. Isolation of Bartonella schoenbuchensis from Lipoptena cervi, a blood-sucking arthropod causing deer-ked dermatitis. Journal of Clinical. Microbiology, 42, 5320–5323. DOI: 10.1128/JCM.42.11.5320-5323.2004.PubMedCrossRefGoogle Scholar
  9. Domański C. (Ed.) 2000. Nieklasyczne metody statystyczne. Polskie Wydawnictwo Ekonomiczne, Warszawa.Google Scholar
  10. Halos L., Jamal T., Maillard R., Girard B., Guillot J., Chomel B., Vayssier-Taussat M., Boulouis H.J. 2004. Role of Hippoboscidae flies as potential vectors of Bartonella spp. infecting wild and domestic ruminants. Applied and Environmental Microbiology, 70, 6302–6305. DOI: 10.1128/AEM.70.10.6302-6305.2004.PubMedCrossRefGoogle Scholar
  11. Kobus P., Pietrzykowski R., Zieliński W. (Eds.). 2001. Statystyka z pakietem STATISTICA. Fundacja Rozwój SGGW, Warszawa, 86–90.Google Scholar
  12. Maillard R., Riegel P., Barrat F., Bouillin C., Thibault D., Gandoin C., Halos L., Demanche C., Alliot A., Guillot J., Piemont Y., Boulouis H.J., Vayssier-Taussat M. 2004a. Bartonella chomelii sp. nov., isolated from French domestic cattle (Bos taurus). International Journal of Systematic and Evolutionary Microbiology, 54, 215–220. DOI: 10.1099/ijs.0.02770-0.PubMedCrossRefGoogle Scholar
  13. Maillard R., Vayssier-Taussat M., Bouillin C., Gandoin C., Halos L., Chomel B., Piemont Y., Boulouis H.J. 2004b. Identification of Bartonella strains isolated from wild and domestic ruminants by a single-step PCR analysis of the 16S–23S intergenic spacer region. Veterinary Microbiology, 1, 63–69. DOI:10.1016/j.vetmic.2003.09.022.CrossRefGoogle Scholar
  14. Morozova O.V., Cabello F.C., Dobrotvorsky AK. 2004. Semi-nested PCR detection of Bartonella henselae in Ixodes persulcatus ticks from Western Siberia, Russia. Vector Borne Zoonotic. Diseases, 4, 306–309.CrossRefGoogle Scholar
  15. Sanogo Y.O., Zeaiter Z., Caruso G., Merola F., Shpynov S., Brouqui P., Raoult D. 2003. Bartonella henselae in Ixodes ricinus ticks (Acari: Ixodida) removed from humans, Belluno Province, Italy. Emerging Infectious Diseases, 9, 329–332.PubMedGoogle Scholar
  16. Schouls L.M., Van De Pol I., Rijpkema S.G., Schot C.S. 1999. Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. Journal of Clinical Microbiology, 37, 2215–2222. DOI: 0095-1137/99/$04.0010.PubMedGoogle Scholar
  17. Schulein R., Seubert A., Gille C., Lanz C., Hansmann Y., Piemont Y., Dehio C. 2001. Invasion and persistent intracellular colonization of erythrocytes: a unique parasitic strategy of the emerging pathogen Bartonella. Journal of Experimental Medicine, 193, 1077–1086. DOI: 10.1084/jem.193.9.1077.PubMedCrossRefGoogle Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  1. 1.Department of Genetics, Faculty of BiologyUniversity of SzczecinSzczecinPoland

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