Acta Parasitologica

, Volume 53, Issue 3, pp 240–250 | Cite as

Gyrodactylus species (Monogenea) infecting alpine bullhead (Cottus poecilopus Heckel, 1837) in Norway and Slovakia, including the description of Gyrodactylus mariannae sp. nov.

  • Anja C. Winger
  • Haakon Hansen
  • Lutz Bachmann
  • Tor A. Bakke
Original Paper

Abstract

Gyrodactylus specimens infecting the skin and fins of two alpine bullhead (Cottus poecilopus) populations from the rivers Signaldalselva (North Norway) and Rena (South-East Norway) were characterized by both morphological and molecular means. Morphometrical differences were minor and the nucleotide sequences of the internal transcribed spacers (ITS) of the nuclear rDNA cluster were identical for parasites from both localities. Based on earlier descriptions, the relatively closest species are Gyrodactylus hrabei Ergens, 1957, described from common bullhead (Cottus gobio) in Slovakia and G. sp. Malmberg, 1973, from alpine bullhead in Sweden. The Norwegian Gyrodactylus specimens from the two alpine bullhead populations were morphometrically different from both the type material of G. hrabei from Slovakia and newly collected Gyrodactylus specimens from alpine bullhead in two Slovakian localities. The Slovakian Gyrodactylus specimens were found to be identical with type material of G. hrabei. The nucleotide sequences of the ITS of the Norwegian Gyrodactylus species were different from the Slovakian material. Hence, the Norwegian Gyrodactylus specimens from the alpine bullhead represent a new species, G. mariannae sp. nov.

Keywords

Monogenea Gyrodactylus hrabei G. mariannae sp. nov Cottus poecilopus species description morphology ribosomal internal transcribed spacer (ITS) 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410. DOI: 10.1016/S0022-2836(05)80360-2.PubMedGoogle Scholar
  2. Andreasson S. 1972. Distribution of Cottus poecilopus Heckel and C. gobio L. (Pisces) in Scandinavia. Zoologica Scripta, 1, 69–78.CrossRefGoogle Scholar
  3. Appleby C. 1996. Variability of the haptoral hard parts of Gyrodactylus callariatis Malmberg, 1957 (Monogenea: Gyrodactylidae) from Atlantic cod Gadus morhua L. in the Oslo Fjord, Norway. Systematic Parasitology, 33, 199–207. DOI: 10.1007/BF01531201.CrossRefGoogle Scholar
  4. Bakke T.A., Harris P.D., Cable J. 2002. Host specificity dynamics: observations on gyrodactylid monogeneans. International Journal for Parasitology, 32, 281–308. DOI: 10.1016/S0020-7519(01)00331-9.PubMedCrossRefGoogle Scholar
  5. Bakke T.A., Cable J., Harris P.D. 2007. The biology of gyrodactylid monogeneans: the “Russian Doll-killers”. Advances in Parasitology, 64, 161–376. DOI: 10.1016/S0065-308X(06)64003-7.PubMedCrossRefGoogle Scholar
  6. Banarescu P. 1990. Zoogeografi of fresh waters. AULA-Verlag, Wiesbaden, 511 pp.Google Scholar
  7. Bush A.O., Lafferty K.D., Lotz J.M., Shostak A.W. 1997. Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, 83, 575–583. DOI: 10.2307/3284227.PubMedCrossRefGoogle Scholar
  8. Cunningham C.O., Mo T.A., Collins C.M., Buchmann K., Thiery R., Blanc G., Lautraite A. 2001. Redescription of Gyrodactylus teuchis Lautraite, Blanc, Thiery, Daniel & Vigneulle, 1999 (Monogenea: Gyrodactylidae); a species identified by ribosomal RNA sequence. Systematic Parasitology, 48, 141–150. DOI: 10.1023/A:1006407428666.PubMedCrossRefGoogle Scholar
  9. Ergens R. 1957. Gyrodactylus hrabei n. sp. s ploutví vranky obecné. Věstník Československé Společnosti Zoologické, 21, 143–146 (In Czech).Google Scholar
  10. Ergens R. 1971. Dactylogyridae and Gyrodactylidae (Monogenoidea) from some fishes from Mongolia. Folia Parasitologica, 18, 241–254.Google Scholar
  11. Ergens R. 1985. Order Gyrodactylidea Bychowsky, 1937. In: (Ed. A. Gusev) Key to the parasites of the freshwater fish fauna of the USSR. Vol. 2. Nauka, Leningrad, 269–347 (In Russian).Google Scholar
  12. Hall T. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
  13. Hansen H., Bachmann L., Bakke T.A. 2003. Mitochondrial DNA variation of Gyrodactylus spp. (Monogenea, Gyrodactylidae) populations infecting Atlantic salmon, grayling, and rainbow trout in Norway and Sweden. International Journal for Parasitology, 33, 1471–1478. DOI: 10.1016/S0020-7519(03)00200-5.PubMedCrossRefGoogle Scholar
  14. Hansen H., Bakke T.A., Bachmann L. 2007. Mitochondrial haplotype diversity of Gyrodactylus thymalli (Platyhelminthes; Monogenea): extended geographic sampling in United Kingdom, Poland, and Norway reveals further lineages. Parasitology Research, 100, 1389–1394. DOI: 10.1007/s00436-006-0423-5.PubMedCrossRefGoogle Scholar
  15. Harris P.D. 1985. Species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) from freshwater fishes in southern England, with a description of Gyrodactylus rogatensis sp. nov. from the bullhead Cottus gobio L. Journal of Natural History, 19, 791–809. DOI: 10.1080/00222938500770491.CrossRefGoogle Scholar
  16. Harris P.D., Cable J., Tinsley R.C., Lazarus C.M. 1999. Combined ribosomal DNA and morphological analysis of individual gyrodactylid monogeneans. Journal of Parasitology, 85, 188–191.PubMedGoogle Scholar
  17. Harris P.D., Shinn A.P., Cable J., Bakke T.A. 2004. Nominal species of the genus Gyrodactylus von Nordmann 1832 (Monogenea: Gyrodactylidae), with a list of principal host species. Systematic Parasitology, 59, 1–27. DOI: 10.1023/B:SYPA.0000038447.52015.e4.PubMedCrossRefGoogle Scholar
  18. Huyse T., Malmberg G. 2004. Molecular and morphological comparisons between Gyrodactylus ostendicus n. sp. (Monogenea: Gyrodactylidae) on Pomatoschistus microps (Kroyer) and G. harengi Malmberg, 1957 on Clupea harengus membras L. Systematic Parasitology, 58, 105–113. DOI: 10.1023/B:SYPA.0000029423.68703.43.PubMedCrossRefGoogle Scholar
  19. Huyse T., Malmberg G., Volckaert F.A.M. 2004. Four new species of Gyrodactylus von Nordmann, 1832 (Monogenea, Gyrodactylidae) on gobiid fishes: combined DNA and morphological analyses. Systematic Parasitology, 59, 103–120. DOI: 10.1023/B:SYPA.0000044427.81580.33.PubMedCrossRefGoogle Scholar
  20. Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120. DOI: 10.1007/BF01731581.PubMedCrossRefGoogle Scholar
  21. Malmberg G. 1964. Taxonomical and ecological problems in Gyrodactylus (Trematoda, Monogenea). In: (Eds. R. Ergens and B. Rysavy) Parasitic Worms and Aquatic Conditions. Publ. House, Czechoslovak Academy of Sciences, Prague, 203–230.Google Scholar
  22. Malmberg G. 1970. The excretory system and the marginal hooks as a basis for the systematics of Gyrodactylus (Trematoda, Monogenea). Arkiv för Zoologi, Ser. 2, 23, 1–235.Google Scholar
  23. Malmberg G. 1973. On a Gyrodactylus species from Northern Sweden and the subgeneric position of G. hrabei Ergens, 1957 (Trematoda, Monogenea). Zoologica Scripta, 2, 39–42. DOI: 10.1111/j.1463-6409.1971.tb00712.x.Google Scholar
  24. Matějusová I., Gelnar M., McBeath A.J.A., Collins C.M., Cunningham C.O. 2001. Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). International Journal for Parasitology, 31, 738–745. DOI: 10.1016/S0020-7519(01)00176-X.PubMedCrossRefGoogle Scholar
  25. Matějusovà I., Gelnar M., Verneau O., Cunningham C.O., Littlewood D.T.J. 2003. Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups. Parasitology, 127, 603–611. DOI: 10.1017/S0031182003004098.PubMedCrossRefGoogle Scholar
  26. Mo T.A. 1993. Seasonal variations of the haptoral hard parts of Gyrodactylus derjavini Mikailov, 1975 (Monogenea: Gyrodactylidae) on brown trout Salmo trutta L. parr and Atlantic salmon S. salar L. parr in the River Sandvikselva, Norway. Systematic Parasitology, 26, 225–231. DOI: 10.1007/BF0009730.CrossRefGoogle Scholar
  27. Nybelin O. 1969. On the immigration of Cottus gobio L. and Cottus poecilopus Haeckel into southern and middle Sweden (in Swedish, with English summary). Acta Regiae Societatis Scientiarum et Litterarum Gothoburgensis, Zoologica, 4, 1–52.Google Scholar
  28. Olstad K. 2008. Taxonomy and systematics in Gyrodactylus von Nordmann, 1832 (Monogenea): Studies on a problematic species complex parasitizing salmonids. PhD Thesis, Faculty of Mathematics and Natural Sciences, University of Oslo (ISSN 1501-7710, Nr. 713).Google Scholar
  29. Pethon P. 2005. Aschehougs store Fiskebok. 5 ed. Aschehoug & Co., Oslo, 468 pp.Google Scholar
  30. Rumyantsev E. A. 2000. Gyrodactylus onegensis sp. n. (Monogenea) — a parasite of the freshwater sculpin (Cottus gobio). Parazitologiya, 34, 156–157.Google Scholar
  31. Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599. DOI: 10.1093/molbev/msm092.PubMedCrossRefGoogle Scholar
  32. Winger A.C. 2004. Taxonomy and systematics of a Gyrodactylus (Monogenea) species infecting Norwegian river populations of alpine bullhead (Cottus poecilopus). Cand. Scient. Thesis in Zoology, Zoological Museum, The Natural History and Botanical Garden, University of Oslo, 1–59.Google Scholar
  33. Winger A.C., Bachmann L., Shinn A., Bakke T.A. 2005. Taxonomy and systematics of Gyrodactylus populations infecting riverine alpine bullhead Cottus poecilopus in Norway and Slovakia. Bulletin of the Scandinavian Society for Parasitology, 14, 161.Google Scholar
  34. Zhang Z., Schwartz S., Wagner L., Miller W. 2000. A greedy algorithm for aligning DNA sequences. Journal of Computational Biology, 7, 203–214. DOI: 10.1089/10665270050081478.PubMedCrossRefGoogle Scholar
  35. Ziętara M.S., Huyse T., Lumme J., Volckaert F.A.M. 2002. Deep divergence among subgenera of Gyrodactylus inferred from rDNA ITS region. Parasitology, 124, 39–52. DOI: 10.1017/S0031182001008939.PubMedGoogle Scholar
  36. Ziętara M.S., Lumme J. 2002. Speciation by host switch and adaptive radiation in a fish parasite genus Gyrodactylus (Monogenea, Gyrodactylidae). Evolution, 56, 2445–2458. DOI: 10.1111/j.0014-3820.2002.tb00170.x.PubMedGoogle Scholar
  37. Ziętara M.S., Lumme J. 2003. The crossroads of molecular, typological and biological species concepts: Two new species of Gyrodactylus Nordmann, 1832 (Monogenea: Gyrodactylidae). Systematic Parasitology, 55, 39–52. DOI: 10.1023/A:1023938415148.PubMedCrossRefGoogle Scholar

Copyright information

© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Anja C. Winger
    • 1
  • Haakon Hansen
    • 3
  • Lutz Bachmann
    • 1
  • Tor A. Bakke
    • 1
  1. 1.Natural History MuseumUniversity of Oslo, Department of ZoologyOsloNorway
  2. 2.Norwegian College of Fishery ScienceUniversity of TromsøTromsøNorway
  3. 3.National Veterinary InstituteSection for ParasitologyOsloNorway

Personalised recommendations