Isolation of the cDNAs encoding secreted and membrane binding proteins from egg of Schistosoma japonicum (Chinese strain)
- 38 Downloads
The granulomatous reaction which occurs around egg trapped in the intrahepatic venules ultimately may lead to fibrosis, which is the main pathogenesis of schistosomiasis. The excreted proteins from eggs play an important role during this process, and they may be a target for developing new strategies to control the hepatic pathogenesis caused by schistosome infection. In this study, fifteen genes encoding secreted or membrane binding protein were identified with the signal sequence trapping method by retrovirus mediated expression screening (SST-REX) of cDNAs from the egg of Schistosoma japonicum (Chinese strain).
KeywordsSchistosoma japonicum secretory or membrane binding protein signal sequence trapping (SST)
Unable to display preview. Download preview PDF.
- Hofmann K., Stoffel W. 1993. TMbase — a database of membrane spanning proteins segments. Biological Chemistry Hoppe-Seyler, 374, 166.Google Scholar
- Liu F., Lu J., Hu W., Wang S.Y., Cui S.J., Chi M., Yan Q., Wang X.R., Song H.D., Xu X.N., Wang J.J., Zhang X.L., Zhang X., Wang Z.Q., Chun-Liang, Brindley P.J., McManus D.P., Yang P.Y., Feng Z., Chen Z., Han Z.G. 2006. New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. PLoS Pathogens, 2, 269–281. DOI: 10.1371/journal.ppat.0020029.CrossRefGoogle Scholar
- Wuhrer M., Balog C.I.A., Catalina MI., Jones F.M., Schramm G., Haas H., Doenhoff M.J., Dunne D.W., Deelder A.M., Hokke C.H. 2006. IPSE/alpha-1, a major secretory glycoprotein antigen from schistosome eggs, expresses the Lewis X motif on core-difucosylated N-glycans. FEBS Journal, 273, 2276–2292. DOI:10.1111/j.1742-4658.2006.05242.x.PubMedCrossRefGoogle Scholar