Cellular & Molecular Biology Letters

, Volume 15, Issue 4, pp 651–664 | Cite as

Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea

  • Hieronim GolczykEmail author
  • Robert Hasterok
  • Marek Szklarczyk
Research Article


High- and low-stringency FISH and base-specific fluorescence were performed on the permanent translocation heterozygote Rhoeo spathacea (2n = 12). Our results indicate that 45S rDNA arrays, rDNA-related sequences and other GC-rich DNA fraction(s) are located within the pericentromeric regions of all twelve chromosomes, usually colocalizing with the chromomycin A3-positive bands. Homogenization of the pericentromeric regions appears to result from the concerted spread of GC-rich sequences, with differential amplification likely. We found new 5S rDNA patterns, which suggest a variability in the breakpoints and in the consequent chromosome reorganizations. It was found that the large 5S rDNA locus residing on each of the 8E and 9E arms consisted of two smaller loci. On each of the two chromosome arms 3b and 4b, in addition to the major subtelomeric 5S rDNA locus, a new minor locus was found interstitially about 40% along the arm length. The arrangement of cytotogenetic landmarks and chromosome arm measurements are discussed with regard to genome repatterning in Rhoeo.

Key words

Pericentromere Permanent translocation heterozygosity rDNA Rhoeo 

Abbreviations used


chromomycin A3 positive


fluorescence in situ hybridization


nucleolus organizer regions


permanent translocation heterozygosity


ribosomal DNA


whole arm translocations


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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2010

Authors and Affiliations

  • Hieronim Golczyk
    • 1
    Email author
  • Robert Hasterok
    • 2
  • Marek Szklarczyk
    • 3
  1. 1.Department of Plant Cytology and Embryology, Institute of BotanyJagiellonian UniversityKrakówPoland
  2. 2.Department of Plant Anatomy and CytologyUniversity of SilesiaKatowicePoland
  3. 3.Department of Genetics, Plant Breeding and Seed ScienceAgricultural University of KrakowKrakówPoland

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