Central European Journal of Biology

, Volume 6, Issue 3, pp 397–404 | Cite as

Biological activity of new flavonoid from Hieracium pilosella L.

  • Monika Gawrońska-GrzywaczEmail author
  • Tadeusz Krzaczek
  • Renata Nowak
  • Renata Los
  • Anna Malm
  • Małgorzata Cyranka
  • Wojciech Rzeski
Research Article


Hieracium pilosella L. (Asteraceae) is a well-known plant used in ethno-medicine as its inflorescences are particularly rich in beneficial polyphenolics. This research aimed to elucidate the structure of a new flavone glycoside isolated from the inflorescences of Hieracium pilosella and evaluate its antioxidant, antimicrobial and antiproliferative activities. The chromatographic methods were successfully applied to isolate the new flavonoid. Its structure was determined by subsequent UV, NMR and MS experiments and identified as isoetin 4′-O-β-D-glucopyranoside. Free radical scavenging capacity was examined by measuring the scavenging activity of the new isoetin derivative on 2,2-diphenyl-1-picrylhydrazyl (DPPH). The compound was also screened for spectrum of antimicrobial activity using the agar well diffusion method. Minimum inhibitory concentration (MIC) for Pseudomonas aeruginosa ATCC 9027 was performed by the micro-dilution broth method. The antiproliferative effect of tested glycoside was assessed in two human tumor cell lines derived from lung (A549) and colon (HT-29) carcinoma and cell proliferation was determined by means of MTT method. The tested compound showed high antiradical activity, reducing the DPPH? with EC50 7.9 μM (3.7 µg/ml) and exhibited narrow antimicrobial spectrum among tested microorganisms. The compound was active against Pseudomonas aeruginosa ATCC 9027 (MIC 125 μg/ml) which is prone to causing infections that are difficult to treat due to it developing extremely rapid antibiotic resistance. In the antiproliferative studies, cell proliferation of the colon (HT-29) carcinoma cell line was significantly decreased after exposure to the compound. The results indicate that isoetin 4′-O-β-D-glucopyranoside possesses antioxidant capacity and very promising antibacterial activity and could have uses as an effective antipseudomonal agent as well a antiproliferative agent.


Hieracium pilosella L. Asteraceae Isoetin 4′-O-β-D-glucopyranoside Antiradical activity DPPH radical Antibacterial activity Pseudomonas aeruginosa Antiproliferative activity HT-29 cell culture MTT method 


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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2011

Authors and Affiliations

  • Monika Gawrońska-Grzywacz
    • 1
    Email author
  • Tadeusz Krzaczek
    • 2
  • Renata Nowak
    • 2
  • Renata Los
    • 3
  • Anna Malm
    • 3
  • Małgorzata Cyranka
    • 4
  • Wojciech Rzeski
    • 4
    • 5
  1. 1.Department of ToxicologyMedical University of LublinLublinPoland
  2. 2.Department of Pharmaceutical BotanyMedical University of LublinLublinPoland
  3. 3.Department of Pharmaceutical MicrobiologyMedical University of LublinLublinPoland
  4. 4.Department of Virology and Immunology, Institute of Microbiology and BiotechnologyMaria Curie-Skłodowska UniversityLublinPoland
  5. 5.Department of Medical BiologyInstitute of Agricultural MedicineLublinPoland

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