Central European Journal of Biology

, Volume 6, Issue 2, pp 160–166 | Cite as

In vitro antifungal activity of propolis samples of Czech and Slovak origin

  • Vladimír BuchtaEmail author
  • Jan Černý
  • Veronika Opletalová
Research Article


Propolis has been used in traditional folk medicine for ages owing to a number of biological effects. Four propolis samples of Czech and one of Slovak origin were extracted using Soxhlet apparatus and analysed by thin-layer chromatography. Raw propolis samples and their extracts were tested by microdilution broth method to determine minimal inhibitory concentration (MIC) in eight strains of human pathogenic fungi. Raw propolis samples showed a lower in vitro antifungal activity than their extracts. In general, the petroleum ether extracts exhibited the highest in vitro antifungal activity (MIC range of 16–64 µg/ml). The content of flavonoids in the samples varied according to region. The highest amount of flavonoids was found in sample A that originated from Broumov (4%). The most susceptible to the propolis extracts were Trichophyton mentagrophytes and Candida albicans. The propolis samples of Czech and Slovak origin and their extracts showed a considerable in vitro antifungal effect which was associated especially with nonpolar petroleum ether and toluene extracts. There was only a partial correlation between flavonoids content and in vitro antifungal activity.


Propolis Flavonoids In vitro antifungal activity Pathogenic fungi TLC Soxhlet extractor 


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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2010

Authors and Affiliations

  • Vladimír Buchta
    • 1
    • 2
    Email author
  • Jan Černý
    • 1
  • Veronika Opletalová
    • 3
  1. 1.Department of Biological and Medical SciencesCharles University in Prague, Faculty of Pharmacy in Hradec KrálovéHradec KrálovéCzech Republic
  2. 2.Department of Clinical MicrobiologyCharles University in Prague, University Hospital and Faculty of Medicine in Hradec KrálovéHradec KrálovéCzech Republic
  3. 3.Department of Pharmaceutical Chemistry and Drug ControlCharles University in Prague, Faculty of Pharmacy in Hradec KrálovéHradec KrálovéCzech Republic

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