New genetic insights to consider coffee waste as feedstock for fuel, feed, and chemicals
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Caffeine is a natural plant product found in many drinks, including coffee, tea, soft and energy drinks. Due to caffeine’s presence in the environment, microorganisms have evolved two different mechanisms to live on caffeine. The genetic maps of the caffeine N-demethylation pathway and C-8 oxidation pathway have been discovered in Pseudomonas putida CBB5 and Pseudomonas sp. CBB1, respectively. These genes are the only characterized bacterial caffeine-degrading genes, and may be of great value in producing fine chemicals, biofuels, and animal feed from coffee and tea waste. Here, we present preliminary results for production of theobromine and 7-methylxanthine from caffeine and theobromine, respectively, by two strains of metabolically engineered E. coli. We also demonstrate complete decaffeination of tea extract by an immobilized mixed culture of Klebsiella and Rhodococcus cells. These processes provide a first level demonstration of biotechnological utilization of coffee and tea waste.
KeywordsCaffeine Coffee waste Pseudomonas putida CBB5 Pseudomonas sp. CBB1 N-demethylase
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- Statistics on Coffee, Historical Data, All exporting countries total production crop years 2010/11 to 2012/13 (International Coffee Organization, London, UK, 2013) http://www.ico.org/historical/2010-19/PDF/TOTPRODUCTION.pdf
- Top 25 agricultural import commodities, with level of processing, by calendar year (United States Department of Agriculture, Washington D.C., USA, 2013) 1Google Scholar
- Data for calendar year commencing: 2011, data sheet (International Coffee Organization, USA, 2011) http://www.ico.org/countries/usa.pdf.
- M.R. Adams, J. Dougan, In: R.J. Clarke. R. Macrae, (Eds.), Waste Products, Coffee: Volume 2. Technology (Elsevier Applied Science Publishers, Ltd, Essex, England, 1987) 257–291Google Scholar
- R.G. Hollingsworth, J.W. Amrstrong, E. Campbell, Nature 361, 1763 (1980)Google Scholar
- A.S. Franca, L.S. Oliveira, In: G.S. Ashworth, P. Azevedo (Eds.), Agricultural Wastes, Coffee processing solid wastes: Current uses and future perspectives (Nova Science Publishers, Inc., New York, U. S. A., 2009) 171–189Google Scholar
- R. Bressani, in: J.E. Braham, R. Bressani (Eds.), Coffee Pulp: Composition, Technology, and Utilization, Potential uses of coffee-berry by-products (International Development Research Centre, Ottawa, Ontario, Canada, 1979) 17–24Google Scholar
- C.A. Woolfolk, J. Bacteriol. 123, 1088 (1975)Google Scholar
- C.A. Woolfolk, J.S. Downard, J. Bacteriol. 130, 1175 (1977)Google Scholar
- S.K. Mohanty, A. Genetic characterization of the caffeine C-8 oxidation pathway in Pseudomonas sp. CBB1. B. Validation of caffeine dehydrogenase as a suitable enzyme for a rapid caffeine diagnostic test, PhD thesis (University of Iowa, Iowa City, Iowa, U.S.A., 2013)Google Scholar
- A.J. Link, D. Phillips, G.M. Church, J. Bacteriol. 179, 6228 (1997)Google Scholar
- W. Seubert, J. Bacteriol. 79, 426 (1960)Google Scholar
- S.R. Gopishetty, T.M. Louie, C.L. Yu. M.V. Subramanian, In: H.N. Thatoi, B.B. Mishra (Eds.), Microbial degradation of caffeine, methylxanthines, and its biotechnological applications, Microbial Biotechnology: Methods and Applications (Narosa Publishing House Pvt, Ltd, New Delhi, India, 2012) 44–67Google Scholar
- S. Roussos, L. Hannibal, M.A. Aquiahuatl. M.R.T. Hernandes, S. Marakis, J. Food Sci. Technol. 31, 316 (1994)Google Scholar