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Central European Journal of Chemistry

, Volume 10, Issue 3, pp 844–875 | Cite as

Comprehensive two-dimensional liquid chromatography — practical impacts of theoretical considerations. A review

  • Pavel Jandera
Review Article

Abstract

A theory of comprehensive two-dimensional separations by liquid chromatographic techniques is overviewed. It includes heart-cutting and comprehensive two-dimensional separation modes, with attention to basic concepts of two-dimensional separations: resolution, peak capacity, efficiency, orthogonality and selectivity. Particular attention is paid to the effects of sample structure on the retention and advantages of a multi-dimensional HPLC for separation of complex samples according to structural correlations. Optimization of 2D separation systems, including correct selection of columns, flow-rate, fraction volumes and mobile phase, is discussed. Benefits of simultaneous programmed elution in both dimensions of LCxLC comprehensive separations are shown.

Experimental setup, modulation of the fraction collection and transfer from the first to the second dimension, compatibility of mobile phases in comprehensive LCxLC, 2D asymmetry and shifts in retention under changing second-dimension elution conditions, are addressed. Illustrative practical examples of comprehensive LCxLC separations are shown.

Keywords

Two-dimensional HPLC Comprehensive LC Orthogonality Selectivity Sample transfer modulation 

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References

  1. [1]
    G. Guiochon, N. Marchetti, K. Mriziq, R.A. Shalliker, J. Chromatogr. A 1189, 109 (2008)CrossRefGoogle Scholar
  2. [2]
    A. Van der Horst, P.J. Schoenmakers, J. Chromatogr. A 1000, 693 (2003)CrossRefGoogle Scholar
  3. [3]
    F. Erni, R.W. Frei, J. Chromatogr. 149, 561 (1978)CrossRefGoogle Scholar
  4. [4]
    M.M. Bushey, J.W. Jorgenson, Anal. Chem. 62, 161 (1990)CrossRefGoogle Scholar
  5. [5]
    J.B. Phillips, J. Beens, J. Chromatogr. A 856, 331 (1999)CrossRefGoogle Scholar
  6. [6]
    P.J. Schoenmakers, P.J. Mariott, J. Beens, LCGC Eur. 16, 335 (2003)Google Scholar
  7. [7]
    O. Amador-Munoz, P.J. Marriott, J. Chromatogr. A 1184, 323 (2008)CrossRefGoogle Scholar
  8. [8]
    K.M. Pierce, J.C. Hoggard, R.E. Mohler, R.E. Synovec, J. Chromatogr. A 1184, 341 (2008)CrossRefGoogle Scholar
  9. [9]
    E. Blahová, P. Jandera, F. Cacciola, L. Mondello, J. Sep. Sci. 29, 555 (2006)CrossRefGoogle Scholar
  10. [10]
    I. Francois, K. Sandra, P. Sandra, Anal. Chim. Acta 641, 14 (2009)CrossRefGoogle Scholar
  11. [11]
    R. Kaiser, Gas Chromstographie (Geest and Portig, Leipzig, 1960) 33Google Scholar
  12. [12]
    M.R. Schure, J. Microcol. Sep. 9, 169 (1997)CrossRefGoogle Scholar
  13. [13]
    K. Horie, H. Kimura, T. Ikegami, A. Iwatsuka, N. Saad, O. Fiehn, N. Tanaka, Anal. Chem., 79, 3764 (2007)CrossRefGoogle Scholar
  14. [14]
    J.C. Giddings, Anal. Chem. 39, 1027 (1967)CrossRefGoogle Scholar
  15. [15]
    P. Jandera, Adv. Chromatogr. 43, 1 (2005)CrossRefGoogle Scholar
  16. [16]
    P. Jandera, J. Churáček, J. Chromatogr. 91, 223 (1974)CrossRefGoogle Scholar
  17. [17]
    L.R. Snyder, J.W. Dolan, J.R. Gant, J. Chromatogr. 165, 3 (1979)CrossRefGoogle Scholar
  18. [18]
    L.R. Snyder, J.W. Dolan, Adv. Chromatogr. 38, 115 (1998)Google Scholar
  19. [19]
    U.D. Neue, J.L. Carmody, Y.F. Cheng, Z. Lu, C.H. Phoebe, T.E. Wheat, Adv. Chromatogr. 41, 93 (2001)Google Scholar
  20. [20]
    P. Jandera, M. Halama, K. Novotná, S. Bunčeková, Chromatographia 57, S153 (2003)CrossRefGoogle Scholar
  21. [21]
    J.M. Davis, J.C. Giddings, Anal. Chem. 55, 418 (1983)CrossRefGoogle Scholar
  22. [22]
    J.M. Davis, J. Chromatogr. A 831, 37 (1999)CrossRefGoogle Scholar
  23. [23]
    J.C. Giddings, In: J.H. Cortes (Ed.), Multidimensional Chromatography: Techniques and Applications (Marcel Dekker, New York, 1990)Google Scholar
  24. [24]
    Chapter 1 J.M. Davis, Anal. Chem. 65, 2014 (1993)CrossRefGoogle Scholar
  25. [25]
    N. Marchetti, A. Felinger, L. Pasti, M.C. Pietrogrande, F. Dondi, Anal. Chem. 76, 3055 (2004)CrossRefGoogle Scholar
  26. [26]
    C.G. Fraga, C.A. Bruckner, R.E. Synovec, Anal. Chem. 73, 675 (2001)CrossRefGoogle Scholar
  27. [27]
    P.J. Slonecker, X. Li, T.H. Ridgway, J.G. Dorsey, Anal. Chem. 68, 682 (1996)CrossRefGoogle Scholar
  28. [28]
    P. Jandera, J. Sep. Sci. 29, 1763 (2006)CrossRefGoogle Scholar
  29. [29]
    H. Pasch, Adv. Polym. Sci. 150, 1 (2000)CrossRefGoogle Scholar
  30. [30]
    M. Gilar, P. Olivova, A.E. Daly, J.C. Gebler, Anal. Chem. 77, 6426 (2005)CrossRefGoogle Scholar
  31. [31]
    H.E. Watson, J.M. Davis, R.E. Synovec, Anal. Chem. 79, 7924 (2007)CrossRefGoogle Scholar
  32. [32]
    P. Jandera, K. Novotná, L. Kolářová, J. Fischer, Chromatographia 60, S27 (2004)CrossRefGoogle Scholar
  33. [33]
    U.D. Neue, J.E. O’.Gara, A. Mendez, J. Chromatogr. A 1127, 161 (2006)CrossRefGoogle Scholar
  34. [34]
    P. Jandera, J. Chromatogr. A 1126, 195 (2006)CrossRefGoogle Scholar
  35. [35]
    P. Jandera, Anal. Chim. Acta 692, 1 (2011)CrossRefGoogle Scholar
  36. [36]
    J.C. Giddings, J. Chromatogr. A 703, 3 (1995)CrossRefGoogle Scholar
  37. [37]
    P. Jandera, M. Holčapek, L. Kolářová, Int. J. Polym. Anal. Charact. 6, 261 (2001)CrossRefGoogle Scholar
  38. [38]
    D. Cho, J. Hong, S. Park, T. Chang, J. Chromatogr. A 986, 199 (2003)CrossRefGoogle Scholar
  39. [39]
    P. Jandera, J. Chromatogr. 314, 13 (1984)CrossRefGoogle Scholar
  40. [40]
    P. Jandera, M. Halama, L. Kolářová, J. Fischer, K. Novotná, J. Chromatogr. A 1087, 112 (2005)CrossRefGoogle Scholar
  41. [41]
    R.E. Murphy, M.R Schure, J.P. Foley, Anal. Chem. 70, 4353 (1998)CrossRefGoogle Scholar
  42. [42]
    P. Jandera, J. Fischer, H. Lahovská, K. Novotná, P. Česla, L. Kolářová, J. Chromatogr. A 1119, 3 (2006)CrossRefGoogle Scholar
  43. [43]
    L. Mondello, P.Q. Tranchida, V. Staněk, P. Jandera, G. Dugo, P. Dugo, J. Chromatogr. A 1086, 91 (2005)CrossRefGoogle Scholar
  44. [44]
    P. Dugo, V. Škeříková, T. Kumm, A. Trozzi, P. Jandera, L. Mondello, Anal. Chem. 78, 7743 (2006)CrossRefGoogle Scholar
  45. [45]
    P. Dugo, M. Herrero, D. Giuffrida, T. Kumm, G. Dugo, L. Mondello, J. Agric. Food Chem. 56, 3478 (2008)CrossRefGoogle Scholar
  46. [46]
    M.J. Gray, G.R. Dennis, P.J. Slonecker, R.A. Shalliker, J. Chromatogr. A 1041, 101 (2004)CrossRefGoogle Scholar
  47. [47]
    G. Vivo-Truyols, P.J. Schoenmakers, J. Chromatogr. A 1120, 273 (2006)CrossRefGoogle Scholar
  48. [48]
    R. Kaliszan, Structure and Retention in Chromatography. A Chemometric Approach (Horwood Academic Publishers, Chichester, 1997)Google Scholar
  49. [49]
    M.H. Abraham, M. Rosés, C.F. Poole, S.K. Poole, J. Phys. Org. Chem. 10, 358 (1997)CrossRefGoogle Scholar
  50. [50]
    R. Kaliszan, Quantitative Structure: Chromatographic Retention Relationship (Wiley, New York, 1987)Google Scholar
  51. [51]
    P. Jandera, K. Vyňuchalová, T. Hájek, P. Česla, G. Vohralík, J. Chemometrics 22, 203 (2008)CrossRefGoogle Scholar
  52. [52]
    D.R. Stoll, P.W. Carr, J. Am. Chem. Soc. 127, 5034 (2005)CrossRefGoogle Scholar
  53. [53]
    P.J. Schoenmakers, G. Vivo-Truyols, W.H.C. Decrop, J. Chromatogr. A 1120, 282 (2006)CrossRefGoogle Scholar
  54. [54]
    H. Poppe, J. Chromatogr. A 778, 3 (1997)CrossRefGoogle Scholar
  55. [55]
    A. Felinger, E. Vigh, A. Gelencser, J. Chromatogr. A 839, 129 (1999)CrossRefGoogle Scholar
  56. [56]
    A.L. Huidobro, P. Pruim, P. Schoenmakers, C. Barbas, J. Chromatogr. A 1190, 182 (2008)CrossRefGoogle Scholar
  57. [57]
    T. Ikegami, T. Hara, H. Kimura, H. Kobayashi, K. Hosoya, K. Cabrera, N. Tanaka, J. Chromatogr. A 1106, 112 (2006)CrossRefGoogle Scholar
  58. [58]
    F. Cacciola, P. Jandera, E. Blahová, L. Mondello, J. Sep. Sci. 29, 2500 (2006)CrossRefGoogle Scholar
  59. [59]
    A. Cavazzini, F. Gritti, K. Kaczmarski, N. Marchetti, G. Guiochon, Anal. Chem. 79, 5972 (2007)CrossRefGoogle Scholar
  60. [60]
    N. Marchetti, A. Cavazzini,, F. Gritti, G. Guiochon, J. Chromatogr. A 1163, 203 (2007)CrossRefGoogle Scholar
  61. [61]
    P. Česla, T. Hájek, P. Jandera, J. Chromatogr. A 1216, 3443 (2009)CrossRefGoogle Scholar
  62. [62]
    K. Deguchi, T. Keira, K. Yamada, H. Ito, Y. Takegawa, H. Nakagawa, S.-I. Nishimura, J. Chromatogr. A 1189, 169 (2008)CrossRefGoogle Scholar
  63. [63]
    Y. Wang, X. Lu, G. Xu, J. Chromatogr. A 1181, 51 (2008)CrossRefGoogle Scholar
  64. [64]
    L.R. Snyder, J.W. Dolan, P.W. Carr, J. Chromatogr. A 1060, 77 (2004)Google Scholar
  65. [65]
    J.W. Dolan, L.R. Snyder, LC GC North America 22, 1146 (2004)Google Scholar
  66. [66]
    N.S. Wilson, M.D. Nelson, J.W. Dolan, L.R. Snyder, R.G. Wolcott, P.W. Carr, J. Chromatogr. A 961, 171 (2002)CrossRefGoogle Scholar
  67. [67]
    J.W. Dolan, L.R. Snyder, J. Chromatogr. A 1216, 3467 (2009)CrossRefGoogle Scholar
  68. [68]
    N.S. Quiming, N.L. Denola, Y. Saito, K. Jinno, Anal. Bioanal. Chem. 388, 1693 (2007)CrossRefGoogle Scholar
  69. [69]
    N.S. Quiming, N.L. Denola, I. Ueta, Y. Saito, S. Tatematsu, K. Jinno, Anal. Chim. Acta 598, 41 (2007)CrossRefGoogle Scholar
  70. [70]
    N.S. Quiming, N.L. Denola, S.R. Bin Samsuri, Y. Saito, K. Jinno, J. Sep. Sci. 31, 1537 (2008)CrossRefGoogle Scholar
  71. [71]
    N.S. Quiming, N.L. Denola, A.B. Soliev, Y. Saito, K. Jinno, Anal. Bioanal. Chem. 389, 1477 (2007)CrossRefGoogle Scholar
  72. [72]
    N.S. Quiming, N.L. Denola, Y. Saito, K. Jinno, J. Sep. Sci. 31, 1550 (2008)CrossRefGoogle Scholar
  73. [73]
    P. Jandera, T. Hájek, V. Škeříková, J. Soukup, J. Sep. Sci. 33, 841 (2010)CrossRefGoogle Scholar
  74. [74]
    P.J. Boersema, N. Divecha, A.J.R. Heck, S. Mohammed, J. Proteome Res. 6, 937 (2007)CrossRefGoogle Scholar
  75. [75]
    R.S. Hodges, Y. Chen, E. Kopecky, C.T. Mant, J. Chromatogr. A 1053, 161 (2004)Google Scholar
  76. [76]
    Y. Wang, R. Lehmann, X. Lu, X. Zhao, G. Xu, J. Chromatogr. A 1204, 28 (2008)CrossRefGoogle Scholar
  77. [77]
    D.L. Callahan, D. Souza, A. Bacic, U. Rossner, J. Sep. Sci. 32, 2273 (2009)CrossRefGoogle Scholar
  78. [78]
    H. Idborg, L. Zamani, P.-O. Edlund, I. Schuppe-Koistinen, S.P. Jacobsson, J. Chromatogr. B 828, 9 (2005)CrossRefGoogle Scholar
  79. [79]
    H. Idborg, L. Zamani, P.-O. Edlund, I. Schuppe-Koistinen, S.P. Jacobsson, J. Chromatogr. B 828, 14 (2005)CrossRefGoogle Scholar
  80. [80]
    N. Takahashi, Y. Wada, J. Awaya, M. Kurono, N. Tomiya, Anal. Biochem. 208, 96 (1993)CrossRefGoogle Scholar
  81. [81]
    N. Takahashi, J. Chromatogr. A 720, 217 (1996)CrossRefGoogle Scholar
  82. [82]
    P. Jandera, J. Churáček, J. Chromatogr. 91, 207 (1974)CrossRefGoogle Scholar
  83. [083]
    T. Kuwana (Ed.), Physical Methods in Modern Chemical Analysis (Academic Press, New York, 1983) vol 3, 249Google Scholar
  84. [84]
    P. Jandera, M. Janderová, J. Churáček, J. Chromatogr. 115, 9 (1975)CrossRefGoogle Scholar
  85. [85]
    P. Jandera, M. Kučerová, J. Chromatogr. A 759, 13 (1997)CrossRefGoogle Scholar
  86. [86]
    P. Jandera, J. Chromatogr. A 965, 239 (2002)CrossRefGoogle Scholar
  87. [87]
    P. Jandera, T. Hájek, J. Sep. Sci. 32, 3603 (2009)CrossRefGoogle Scholar
  88. [88]
    J. Urban, V. Škeříková, P. Jandera, R. Kubíčková, M. Pospíšilová, J. Sep. Sci. 32, 2530 (2009)CrossRefGoogle Scholar
  89. [89]
    G.J. Opiteck, J.W. Jorgenson, R.J. Anderegg, Anal. Chem. 69, 2283 (1997)CrossRefGoogle Scholar
  90. [90]
    K.J. Mayfield, R.A. Shaliker, H.J. Catchpoole, A.P. Sweeney, V. Wong, G. Guiochon, J. Chromatogr. A 1080, 124 (2005)CrossRefGoogle Scholar
  91. [91]
    P. Dugo, O. Favoino, R. Lupino, G. Dugo, L. Mondello, Anal. Chem. 76, 2525 (2004)CrossRefGoogle Scholar
  92. [92]
    P. Dugo, O. Favoino, P.Q. Tranchida, G. Dugo, L. Mondello, J. Chromatogr. A 1041, 135 (2004)CrossRefGoogle Scholar
  93. [93]
    H. Tian, J. Xu, Y. Xu, Y. Guan, J. Chromatogr. A 1137, 42 (2006)CrossRefGoogle Scholar
  94. [94]
    P. Dugo, T. Kumm, M.L. Crupi, A. Cotroneo, L. Mondello, J. Chromatogr. A 1112, 269 (2006)CrossRefGoogle Scholar
  95. [95]
    P. Jandera, Anal. Chim. Acta 692, 1 (2011)CrossRefGoogle Scholar
  96. [96]
    P. Jandera, J. Separation Sci. 31, 1421 (2008)CrossRefGoogle Scholar
  97. [97]
    S.R. Wilson, M. Jankowski, M. Pepaj, A. Mihailova, F. Boix, G. Truyols-Vivo, E. Lundanes, T. Greibrokk, Chromatographia 66, 469 (2007)CrossRefGoogle Scholar
  98. [98]
    J.C. Giddings, In H.J. Cortes (Ed.), Multidimensional Chromatography — Techniques and Applications (Marcel Dekker, New York, 1990)Google Scholar
  99. [99]
    W. Khummueng, P.J. Marriott, LC GC Europe 22, 38 (2009)Google Scholar
  100. [100]
    R.E. Murphy, M.R. Schure, J.P. Foley, Anal. Chem. 70, 1585 (1998)CrossRefGoogle Scholar
  101. [101]
    A.P. Schellinger, P.W. Carr, J. Chromatogr. A 1077, 110 (2005)CrossRefGoogle Scholar
  102. [102]
    A.P. Schellinger, D.R. Stoll, P.W. Carr, J. Chromatogr. A 1064, 143 (2005)CrossRefGoogle Scholar
  103. [103]
    L.W. Potts, D.R. Stoll, X. Li, P.W. Carr, J. Chromatogr. A 1217, 5700 (2010)CrossRefGoogle Scholar
  104. [104]
    P.W. Carr; D.R. Stoll, X. Wang, Anal. Chem. 83, 1890 (2011)Google Scholar
  105. [105]
    X.P. Li, D.R. Stoll, P.W. Carr, Anal. Chem. 81, 845 (2009)CrossRefGoogle Scholar
  106. [106]
    M. Kivilompolo, T. Hyotylainen, J. Sep. Sci. 31, 3466 (2008)CrossRefGoogle Scholar
  107. [107]
    K. Broeckhoven, D. Cabooter, F. Lynen, P. Sandra, G. Desmet, J. Chromatogr. A 1217, 2787 (2010)CrossRefGoogle Scholar
  108. [108]
    H. Gu, Y. Huang, P.W. Carr, J. Chromatogr. A 1218, 64 (2011)CrossRefGoogle Scholar
  109. [109]
    P. Jandera, P. Česla, T. Hájek, G. Vohralík, K. Vyňuchalová, J. Fischer, J. Chromatogr. A 1189, 207 (2008)CrossRefGoogle Scholar
  110. [110]
    P. Jandera, T. Hájek, P. Česla, J. Sep. Sci. 33, 1362 (2010)Google Scholar
  111. [111]
    P. Jandera, M. Halama, K. Novotná, J. Chromatogr. A 1030, 33 (2004)CrossRefGoogle Scholar
  112. [112]
    U.D. Neue, J.R. Mazzeo, J. Sep. Sci. 24, 921 (2001)CrossRefGoogle Scholar
  113. [113]
    P. Jandera, J. Chromatogr. A 352, 91 (1986)CrossRefGoogle Scholar
  114. [114]
    P. Česla, T. Hájek, P. Jandera, J. Chromatogr. A 1216, 3443 (2009)CrossRefGoogle Scholar
  115. [115]
    F. Cacciola, P. Jandera, Z. Hajdú, P. Česla, L. Mondello, J. Chromatogr. A 1149, 73 (2007)CrossRefGoogle Scholar
  116. [116]
    C.G. Fraga, B.J. Prazen, R.E. Synovec, Anal. Chem. 73, 5833 (2001)CrossRefGoogle Scholar
  117. [117]
    T. Skov, J.C. Hoggard, R. Bro, R.E. Synovec, J. Chromatogr. A 1216, 4020 (2009)CrossRefGoogle Scholar

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© © Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of Chemical TechnologyUniversity of PardubicePardubiceCzech Republic

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