Central European Journal of Chemistry

, Volume 2, Issue 1, pp 82–90 | Cite as

Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection

  • P. Niedzielski
  • M. Siepak
  • K. Novotny


The paper presents the principles and advantages of a technique combining high performance liquid chromatography and hydride generation atomic absorption spectrometry (HPLC-HGAAS) applied to speciation analysis of inorganic species of arsenic As(III) and As(V) in ground water samples. With separation of the arsenic species on an ion-exchange column in the chromatographic system and their detection by the hydride generation atomic absorption spectrometry, the separation of the analytical signals of the arsenic species was excellent at the limits of determination of 1.5 ng/ml As(III) and 2.2 ng/ml As(V) and RSD of 4.3% and 7.8% for the concentration of 25 ng/ml. The hyphenated technique has been applied for determination of arsenic in polluted ground water in the course of the study on migration of micropollutants. For total arsenic concentration two independent methods: HGICP-OES and HGAAS were used for comparison of results of real samples analysis.


speciation hyphenated techniques arsenic hydride generation absorption atomic spectrometry high performance liquid chromatography 


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Copyright information

© Central European Science Journals 2004

Authors and Affiliations

  • P. Niedzielski
    • 1
  • M. Siepak
    • 2
  • K. Novotny
    • 3
  1. 1.Department of Water and Soil AnalysisAdam Mickiewicz UniversityPoznańPoland
  2. 2.Department of Hydrogeology and Waters ProtectionAdam Mickiewicz UniversityPoznańPoland
  3. 3.Laboratory of Atomic SpectrochemistryMasaryk UniversityBrnoCzech Republic

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