Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection
- 101 Downloads
The paper presents the principles and advantages of a technique combining high performance liquid chromatography and hydride generation atomic absorption spectrometry (HPLC-HGAAS) applied to speciation analysis of inorganic species of arsenic As(III) and As(V) in ground water samples. With separation of the arsenic species on an ion-exchange column in the chromatographic system and their detection by the hydride generation atomic absorption spectrometry, the separation of the analytical signals of the arsenic species was excellent at the limits of determination of 1.5 ng/ml As(III) and 2.2 ng/ml As(V) and RSD of 4.3% and 7.8% for the concentration of 25 ng/ml. The hyphenated technique has been applied for determination of arsenic in polluted ground water in the course of the study on migration of micropollutants. For total arsenic concentration two independent methods: HGICP-OES and HGAAS were used for comparison of results of real samples analysis.
Keywordsspeciation hyphenated techniques arsenic hydride generation absorption atomic spectrometry high performance liquid chromatography
Unable to display preview. Download preview PDF.
- M.A. Suner, V. Devesa, I. Rivas, D. Velez and R.J. Montoro: “Application of column switching in high performance liquid chromatography with on line thermo oxidation and detection by HG AAS and HG AFS for the analysis of organoarsenical species in seafood samples”, J. Anal. At. Spectrom., Vol. 16, (2001), pp. 390–397.CrossRefGoogle Scholar
- P. Niedzielski, M. Siepak, J. Przybyłek and J. Siepak: “Arsenic, antimony and selenium in water city of Poznań”, Betagraf, Poznań, 2002, (in Polish).Google Scholar
- P. Niedzielski, M. Siepak, J. Siepak and J. Przybyłek: “Determination of different forms of arsenic antimony and selenium in water samples using hydride generation”, Polish. Pol. J. Environ. Stud. Vol. 11, (2002), pp. 219–224.Google Scholar
- D.L. Tsalev, M. Sperling and B. Welz: “Flow-injection hydride generation atomic absorption spectrometric study of the automated on-line pre-reduction of arsenate, methylarsonate and dimethylarsinate and high-performance liquid chromatographic separation of their L-cysteine complexes”, Talanta, Vol. 51, (2000), pp. 1059–1068.CrossRefGoogle Scholar
- D.L. Tsalev, M. Sperling and B. Welz: “On-line UV-photooxidation with peroxodisulfate forautomated flow injection and for high-performanceliquid chromatography coupled to hydride generationatomic absorption spectrometry”, Spectrochimica Acta Part B, Vol. 55, (2000), pp. 3390–353.Google Scholar
- M.C. Villa-Lojo, E. Alonso-Rodriguez, P. Lopez-Mahia, S. Muniategui-Lorenzo and D. Prada-Rodriguez: “Coupled high performance liquid chromatography-microwave digestion-hydride generation-atomic absorption spectrometry for inorganic and organic arsenic speciation in fish tissue”, Talanta, Vol. 57, (2002), pp. 741–750.CrossRefGoogle Scholar
- W.Ch. Tseng, M.H. Yang, T.P. Chen and Y.L. Huang: “Automated, continuous, and dynamic speciation of urinary arsenic in the bladder of living organisms using microdialysis sampling coupled on-line with high performance liquid chromatography and hydride generation atomic absorption spectrometry”, Analyst, Vol. 127, (2002), pp. 560–564.CrossRefGoogle Scholar
- P. Niedzielski, J. Siepak and M. Siepak: “Total Content of Arsenic, Antimony and Selenium in Groundwater Samples from Western Poland”, Pol. J. Environ. Stud., Vol. 5, (2001), pp. 347–350.Google Scholar