Estuaries

, Volume 7, Issue 4, pp 506–512 | Cite as

Nutrient limitation of phytoplankton growth in Georgia nearshore waters

  • S. Stephen Bishop
  • Kurt A. Emmanuele
  • James A. Yoder
Article

Abstract

Nutrient enrichment experiments were conducted to investigate the utilization of dissolved organic and inorganic nitrogen by marine phytoplankton in Georgia coastal waters. Natural populations of marine phytoplankton, enriched with different concentrations of ammonium chloride and other plant nutrients, were grown under controlled temperature and irradiance conditions until the populations reached “stationary phase.” Results showed that (1) phytoplankton are limited by DIN up to ca. 20μM, when another nutrient (phosphate or silicate) becomes limiting, (2) very little naturally-occurring DON is directly utilized for growth, (3) very little DON is indirectly made available for growth over time periods of days to ca. 1 week, and (4) trace metals and vitamins do not significantly limit phytoplankton growth.

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Literature Cited

  1. Antia, N. J., B. R. Berland, D. J. Donin, andS. Y. Maestrini. 1975. Comparative evaluation of certain organic and inorganic sources of nitrogen for phototrophic growth of marine microalgae.J. Mar. Biol. Assoc. U.K. 55:519–539.Google Scholar
  2. Bishop, S. S., J. A. Yoder, andG.-A. Paffenhofer. 1980. Phytoplankton and nutrient variability along a cross-shelf transect off Savannah, Georgia, U.S.A.Estuarine Coastal Mar. Sci. 11:359–368.CrossRefGoogle Scholar
  3. Dugdale, R. C., andJ. J. Goering 1967. Uptake of new and regenerated forms of nitrogen in primary productivity.Limnol. Oceanogr., 12:196–206.Google Scholar
  4. Dunstan, W. M., andL. P. Atkinson. 1976. Sources of new nitrogen for the South Atlantic Bight, p. 69–78.In M. Wiley (ed.), Estuarine Processes, Vol.1. Academic Press, New York.Google Scholar
  5. Glibert, D. L., and L. C. Loder. 1977. Automated analysis of nutrients in seawater: a manual of techniques. Woods Hole Oceanographic Institute, Technical Report, WHOI-77-47.Google Scholar
  6. Guillard, R. R. L., andJ. H. Ryther. 1962. Studies of marine planktonic diatoms.Can. J. Microbiol. 8:229–239.Google Scholar
  7. Guillard, R. R. L. 1963. Organic sources of nitrogen for marine centric diatoms, p. 93–104.In C. H. Oppenheimer (ed.), Symposium of Marine Microbiology. C. C Thomas, Springfield, Illinois.Google Scholar
  8. Haines, E. B. 1974. Processes affecting production in Georgia coastal waters. Ph.D. Thesis, Duke University, Durham, North Carolina.Google Scholar
  9. Haines, E. B. 1979. Nitrogen pools in Georgia coastal waters.Estuaries 2:34–39.CrossRefGoogle Scholar
  10. Harvey, H. W. 1940. Nitrogen and phosphorous required for the growth of phytoplankton.J. Mar. Biol. Assoc. U.K. 24:115–123.CrossRefGoogle Scholar
  11. Hayward, J. 1965. Studies on the growth ofPhaeodactylum tricornutum (Bohlin).Physiol. Plant. 18: 201–207.CrossRefGoogle Scholar
  12. Kierfve, B., andH. N. McKellar, Jr. 1980. Time series measurements of estuarine material fluxes, p. 341–358.In V. S. Kennedy (ed.), Estuarine Perspectives. Academic Press, New York.Google Scholar
  13. Lee, T. N., L. P. Atkinson, andR. Legeckis. 1981. Observations of a Gulf Stream frontal eddy on the Georgia continental shelf, April 1977.Deep-Sea Res. 28:347–378.CrossRefGoogle Scholar
  14. Lee, T. N., andL. P. Atkinson. 1983. Low frequency current and temperature variability from Gulf Stream frontal eddies and atmospheric forcing along the southeastern U.S. outer continental shelf.J. Geophys. Res. 88:4541–4567.CrossRefGoogle Scholar
  15. Lui, N. S. T., andO. A. Roels. 1970. Nitrogen metabolism of aquatic organisms.Arch. Biochem. Biophys. 139:269–277.CrossRefGoogle Scholar
  16. McCarthy, J. J. 1972. The uptake of urea by natural populations of marine phytoplankton.Limnol. Oceanogr. 17:738–748.Google Scholar
  17. McCarthy, J. J. 1980. Nitrogen, p. 191–233.In I. Morris (ed.), The Physiological Ecology of Phytoplankton. University of California Press, Berkeley.Google Scholar
  18. Oertel, G. F., andW. M. Dunstan. 1981. Suspended sediment and certain aspects of phytoplankton production off Georgia, U.S.A..Mar. Geol. 40: 171–197.CrossRefGoogle Scholar
  19. Pinter, D. J., andL. Provasoli. 1963. Nutritional characteristics of some chrysomonads, p. 114–121.In C. H. Oppenheimer (ed.), Symposium on Marine Microbiology, C. C. Thomas, Springfield, Illinois.Google Scholar
  20. Remsen, C. C. 1971. The distribution of urea in coastal and oceanic waters.Limnol. Oceanogr. 16:732–740.Google Scholar
  21. Ryther, J. H. 1954. The ecology of phytoplankton blooms in Moriches Bay and Great South Bay, Long Island, New York.Biol. Bull. 106:198–209.CrossRefGoogle Scholar
  22. Solorzano, L., andJ. H. Sharp. 1980. Determination of total dissolved nitrogen in natural waters.Limnol. Oceanogr. 25:751–754.Google Scholar
  23. Strickland, J. D. H., andT. R. Parsons. 1972. A practical handbook of seawater analysis. Bulletin 167. Fisheries Research Board of Canada, Ottawa.Google Scholar
  24. Syrett, P. J. 1962. Nitrogen assimilation, p. 171–188.In R. A. Lewin (ed.), Physiology and Biochemistry of Algae. Academic Press, New York.Google Scholar
  25. Thomas, J. P. 1966. Influence of the Altamaha River on primary production beyond the mouth of the river. M.S. Thesis, University of Georgia, Athens. 88 p.Google Scholar
  26. Turner, R. E. 1981. Plankton productivity and the distribution of fishes on the southeastern U.S. continental shelf.Science 214:351–354.CrossRefGoogle Scholar
  27. Vaccaro, R. F. 1963. Available nitrogen and phosphorus and the biochemicla cycle in the Atlantic off New England.J. Mar. Res. 21:284–301.Google Scholar
  28. Wheeler, P. A., B. B. North, andG. C. Stephens. 1974. Amino acid uptake by marine phytoplankton.Limnol. Oceanogr. 19:249–259.CrossRefGoogle Scholar
  29. Yentsch, C., andD. W. Menzel. 1963. A method for the determination of phytoplankton, chlorophyll and phaeophytin by fluorescence.Deep-Sea Res. 10: 221–231.Google Scholar
  30. Yoder, J. A. (In press). Environmental control of phytoplankton production on the southeastern U.S. continental shelf.In D. W. Menzel and L. P. Atkinson (eds.), Oceanography of the South Atlantic Bight. American Geophysical Union, Washington, D.C.Google Scholar
  31. Zar, J. H. 1974. Biostatistical Analyses. Prentice-Hall, Englewood Cliffs, New Jersey. 620 p.Google Scholar

Copyright information

© Estuarine Research Federation 1984

Authors and Affiliations

  • S. Stephen Bishop
    • 1
  • Kurt A. Emmanuele
    • 1
  • James A. Yoder
    • 1
  1. 1.Skidaway Institute of OceanographySavannah

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