Gregarious setting in the American oysterCrassostrea virginica Gmelin: I. Properties of a partially purified “Setting factor”
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A substance which stimulates the gregarious setting of oyster larvae has been partially purified. This material, which is present in the oyster shell liquor, is a protein of molecular weight in excess of 100,000 Daltons. Thyroxine, and possibly 3,5-diiodotyrosine and 3-iodotyrosine are freed by enzymatic or alkaline hydrolysis of the protein. These same substances are associated with the biological activity as they too will stimulate setting of larvae, although much higher concentrations are necessary for activity comparable to that of the conjugate protein.
KeywordsThyroxine Crassostrea Virginica Glass Distil Water American Oyster Chesapeake Biological Laboratory
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- BLOCK, R. J., E. L. DURRUM, and G. ZWEIG. 1958. Paper chromatography and paper electrophoresis, 2nd Ed., p. 138–154. Academic Press, N. Y.Google Scholar
- COLE, H. A., and E. W. KNIGHT-JONES. 1949. The setting behavior of larvae of the European oyster,Ostrea edulis L., and its influence on methods of cultivation and spat collection.Fish. Invest., Lond. Ser. II, 17(3): 39 p.Google Scholar
- CRISP, D. J. 1965. Surface chemistry, a factor in the settlement of marine invertebrate larvae. Botanica Gothoburgensia III, Proc. of the Fifth Marine Biological Symposium, Goteborg, 1965, p. 51–65.Google Scholar
- —, F. P. VEITCH, and P. E. O’BRIEN. 1969. Gregarious setting in the American oyster.Proc. Natl. Shellfish Assn., 60:4 (abstr.).Google Scholar
- KECK, R., D. MAUER, J. KAUER, and W. SHEPPARD. Chemical stimulants to larval settlement in the American oyster.Proc. Natl. Shellfish Assn. (In press).Google Scholar
- MEYER, F. 1938.Cold Spring Harbor Symposia Quant. Biol., 6:91.Google Scholar
- ROCHE, J., S. LISSITZKY, and A. MICHEL. 1954. Methods of Biochemical Analysis, Editor David Glick. Interscience Publishers, Inc., New York. p. 244.Google Scholar
- SPIRO, R. G., and M. J. Spiro. 1965. The carbohydrate composition of the thyroglobulins from several species. J. Biol. Chem. 240:997.Google Scholar